Study on the molecular biological diagnosis of male infertility

阐明生精机制和生精障碍对男性不育症的诊断和治疗具有重要意义。在本研究中，利用分子生物学技术进行了以下实验。目的：1.从人睾丸cDNA文库中克隆人FSH受体基因。男性不育患者的DNA分析(A)在40例无精子症患者中，有6例发现Y染色体特异DNA的微小缺失，该DNA可能与精子发生有关。(2)20例无精子症患者均未发现雄激素受体基因缺失。(3)10例无精子症患者均未发现FSH受体基因缺失。不育患者雄激素和卵泡刺激素受体基因表达的分析(A)在10例无精子症患者中，有4例睾丸FSH受体减少。(B)在无精子症患者中，已测量了雄激素受体，目前正在进行分析。

It is very important for diagnosis and treatment of male infertility to clarify the mechanism and disorders of spermatogenesis. In the present research, the following experiments were performed using molecular biological techniques.1. Human FSH receptor cDNA was isolated from human testis cDNA library.2. DNA analysis of male infertility patients (A)In 6 out of 40 azoospermic patients, a minute deletion of Y chromosome specific DNA, which was suspected to be related to spermatogenesis, was found. (B)In all 20 azoospermic patients examined, no deletion of androgen receptor gene was found. (C)In all 10 azoospermic patients examined, no deletion of FSH receptor gene was found.3. Analysis of gene expression of androgen and FSH receptors in infertile patients (A)In 4 out of 10 azoospermic patients examined, testicular FSH receptors were decreased. (B)In azoospermic patients, androgen receptors were measured and now under analysis.