MECHANISM OF REGULATION OF TELOMERASE EXPRESSION, AND EXPERIMENTAL STUDY ON CANCER GENE THERAPY USING ITS PROMOTER.

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Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase, which is highly active in immortalized cells. We herein carried out basic experiments on cancer gene therapy utilizing the hTERT promoter. The core promoter region was identified by deletion analysis, and demethylation of this region accelerated the expression of p16 and E-cadherin. Cytosine deaminase gene was ligated in downstream of hTERT promoter. In vitro experiments demonstrated that cell- kill effect was only seen in cancer cells not in normal cells. Tandem repeat promoter is now under construction for creating more active and specific tool in gene therapy. Adenovirus vector containing hTERT promoter and CD was constructed, and used in vivo experiments. The data was promising and could be widely utilized in the field of cancer gene therapy because of its specificity.