Solution of the molecular mechanism about proliferation / recurrence of the prostate cancer and the architecture of the comprehensive treatment strategy for he prostate cancer

解决前列腺癌增殖/复发的分子机制及前列腺癌综合治疗策略架构

• 批准号: 23390379
• 负责人: NAMIKI Mikio
• 金额: $ 3.49万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-11-18 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390379/
• 关键词: 前立腺癌; SOD3; SPARC; フラボノイド; 共培養; 再燃前立腺癌; ｱﾝﾄﾞﾛｹﾞﾝ; 微小環境; 間質細胞; エストロゲン; cDNA microarray

We performed cDNA microarray analysis with normal prostate tissue and the prostate cancer tissue, and identified SOD3 as the gene which the expression attenuated with prostate cancer tissue.We revealed that expression diminishment of SOD3 was associated with proliferation of prostate cancer, migration, and invasion. Also, we paid attention to extracellular matrix SPARC. Expression of SPARC attenuated with prostate cancer-derived stromal cells than normal stromal cells, and inhibited a proliferation and the migration of the cancer cells by inhibiting AKT phosphorylation. SPARC acted on cancer cells via integrin beta 1 as a receptor. Also, 2'-hydroxyflavanone which is the plant flavonoid showed antitumor effect for prostate cancer via apoptosis, and revealed that 2'-hydroxyflavanone inhibited androgen receptor activity, but did not inhibit androgen synthesis.

我们对正常前列腺组织和前列腺癌组织进行了cDNA微阵列分析，发现SOD 3是前列腺癌组织中表达减弱的基因，我们发现SOD 3的表达减弱与前列腺癌的增殖、迁移和侵袭有关。同时，我们也关注了细胞外基质的变化。前列腺癌间质细胞中AKT的表达比正常间质细胞减弱，并通过抑制AKT磷酸化抑制癌细胞的增殖和迁移。它通过整合素β 1作为受体作用于癌细胞。此外，作为植物类黄酮的2 '-羟基黄烷酮通过细胞凋亡显示出对前列腺癌的抗肿瘤作用，并且揭示了2'-羟基黄烷酮抑制雄激素受体活性，但不抑制雄激素合成。

项目成果

SPARC Suppresses Proliferation and Migration of LNCaP Human prostate cancer cell

SPARC 抑制 LNCaP 人前列腺癌细胞的增殖和迁移

• 发表时间: 2012
• 作者: Shin M;et al
• 通讯作者: et al

Repression of cell proliferation and androgen receptor activity in prostate cancer cells by 2'-hydroxyflavanone

2-羟基黄烷酮抑制前列腺癌细胞的细胞增殖和雄激素受体活性

• 发表时间: 2013
• 期刊: Anticancer Research
• 影响因子: 2
• 作者: M. Ofude;A. Mizokami;M. Kumaki;K. Izumi;H. Konaka;Y. Kadono;Y. Kitagawa;M. Shin;J. Zhang;E. T. Keller and M. Namiki
• 通讯作者: E. T. Keller and M. Namiki

SOD3 Overexpression Inhibits Growth and Migration in PC-3 Human Prostate Cancer Cells

SOD3 过表达抑制 PC-3 人前列腺癌细胞的生长和迁移

• 发表时间: 2014
• 作者: Jungim Kim;et al
• 通讯作者: et al