Expression of collagenase and collagenase inhibitors mRNA in periodontitisaffected human gingival tissue.

胶原酶和胶原酶抑制剂 mRNA 在牙周炎影响的人牙龈组织中的表达。

• 批准号: 03454440
• 负责人: YOSHIE Hiromasa
• 金额: $ 4.22万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454440/
• 关键词: TIMP-gene; Collagenase-gene; Periodontitis; RT-PCR; 逆転写PCR法; 歯肉; コラゲナ-ゼ; TIMP

Collagenolysis in periodontitis is thought to be modulated by the expression of three genes, collagenase, tissue inhibitors of metalloproteinases-1 and -2 (TIMP-1 and-2). We assessed the possible difference in TIMP-1, TIMP-2 and collagenase mRNA levels between gingival samples from patients with periodontitis and those from healthy subjects by reverse transcription-polymerase chain reaction (RT- PCR). This technique allows detection of transcripts from a very small sample quantity. The experiments showed that levels of TIMP-1 and collagenase transcripts relative to beta-actin are significantly higher in the diseased group than in health controls (8.11*0.83 versus 1.38*0.28% for TIMP-1 and 0.50*0.10 versus 0.0075*0.0024% for collagenas, respectively). The difference in TIMP-2 between the two groups(2.91*0.46 versus 1.84*0.87%) did not differ. Therefore, the host would have responded to the increase in collagenase level by preferentially producing TIMP-1 against tissue destruction. The differential gene expression of TIMP-1 and TIMP-2 in our study may account for a distinct genetic regulation of TIMP-1 and -2 in vivo.

牙周炎的胶原溶解被认为是由三个基因的表达调节的，胶原酶，金属蛋白酶组织抑制剂-1和-2 （TIMP-1和2）。我们通过逆转录聚合酶链反应（RT- PCR）评估牙周炎患者和健康受试者牙龈样品中TIMP-1、TIMP-2和胶原酶mRNA水平的可能差异。这种技术允许从非常小的样本量中检测转录本。实验表明，患病组的TIMP-1和胶原酶转录物相对于β -肌动蛋白的水平显著高于健康对照组（TIMP-1为8.11*0.83比1.38*0.28%，胶原为0.50*0.10比0.0075*0.0024%）。两组间TIMP-2差异无统计学意义（2.91*0.46 vs 1.84*0.87%）。因此，宿主会通过优先产生TIMP-1来对抗组织破坏，从而对胶原酶水平的增加做出反应。在我们的研究中，TIMP-1和TIMP-2的差异基因表达可能解释了TIMP-1和-2在体内的不同遗传调控。

项目成果

野村 隆,高橋 徳也,原 耕二: "ヒト歯周炎罹患者歯肉におけるTIMP-1,TIMP-2かよびCollagenase mRNA発現の検索" 新潟歯学会雑誌. 22. 71-83 (1992)

Takashi Nomura、Tokuya Takahashi、Koji Hara：“搜索人类牙周炎患者牙龈中的 TIMP-1、TIMP-2 和胶原酶 mRNA 表达”《新泻牙科学会杂志》22. 71-83 (1992)。

Takashi NOMURA, Tokuya TAKAHASHI and Kohji HARA: "Expression of TIMP-1,TIMP-2 and collagenase mRNA in periodontitis-affected human gingival tissue." Niigata Dental Journal. Vol. 22. 71-83 (1992)

Takashi NOMURA、Tokuya TAKAHASHI 和 Kohji HARA：“TIMP-1、TIMP-2 和胶原酶 mRNA 在牙周炎影响的人类牙龈组织中的表达。”

Takashi NOMURA,Tokuya TAKAHASHI,Kohji HARA: "Expression of TIMP-1,TIMP-2 and Collagenase mRNA im Periodontitis-affected Human Gingival Tissue" Journal of Periodontal Research.

Takashi NOMURA、Tokuya TAKAHASHI、Kohji HARA：“TIMP-1、TIMP-2 和胶原酶 mRNA 在牙周炎影响的人牙龈组织中的表达”牙周研究杂志。

Takashi NOMURA, Tokuya TAKAHASHI and Kohji HARA.: "Expression of TIMP-1, TIMP-2 and collagenase mRNA in periodontitis-affected human gingival tissue." Journal of Periodontal Research.

Takashi NOMURA、Tokuya TAKAHASHI 和 Kohji HARA.：“TIMP-1、TIMP-2 和胶原酶 mRNA 在牙周炎影响的人类牙龈组织中的表达。”

野村 隆 高橋 徳也 原 耕二: "ヒト歯周炎罹患歯肉におけるTIMP-1,TIMP-2およびCollagenase mRNA発現の検索" 新潟歯学会雑誌. 22. 71-83 (1992)

Takashi Nomura、Tokuya Takahashi 和 Koji Hara：“搜索受牙周炎影响的牙龈中的 TIMP-1、TIMP-2 和胶原酶 mRNA 表达”Niigata Dental Society Journal 22. 71-83 (1992)。