Bone homeostasis-related genes associated with susceptibility to periodicities

与周期性易感性相关的骨稳态相关基因

• 批准号: 13470461
• 负责人: YOSHIE Hiromasa
• 金额: $ 10.56万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470461/
• 关键词: Periodontitis; Bone homeostasis; Gene polymorphism; VDR; IL-1RA; TNFR; FcγR; CD14; FcγRIIb; 感受性遺伝子; 1塩基多型; TNF-α

Vitamin D receptor (VDR), Tumor necrosis factor alpha (TNF-α), TNF receptor, Interleukin-1 (IL-1), IL-1 receptor antagonist (IL-1RA), and CD14 had been reported to be related with bone homeostasis and were selected as candidate genes in this study. Association between polymorphisms in the candidate genes and susceptibility to aggressive or chronic periodontitis were assessed in case-control studies. Genotypes were determined using genomic DNA obtained from Japanese patients and healthy controls.In Japanese subjects, the polymorphism in the 5'-flanking region of TNF-α gene was not associated with susceptibility to generalized aggressive periodontitis (G-AgP), whereas the combination of genotypes of VDR and IgG receptor, FcγRIIIb, and VNTR (variable number of tandem repeats) in IL-1RA gene showed significant associations with the susceptibility to G-AgP. Single nucleotide polymorphisms (SNPs) in TNFR1 and FcγRIIb genes were associated with G-AgP, respectively. A polymorphism in CD14 gene was not related to the development of periodontitis, but suggested to be related to early disease activity.These analyses demonstrated that a number of genes related to bone homeostasis were relevant with the development of periodontitis as combinatorial factors. The findings in this study may be useful for a diagnosis system with multiple genetic factors of periodontitis.

维生素D受体（VDR）、肿瘤坏死因子-α（TNF-α）、肿瘤坏死因子受体（TNF-α）、白细胞介素-1（IL-1）、IL-1受体拮抗剂（IL-1 RA）和CD 14等与骨稳态相关的基因被作为本研究的候选基因。在病例对照研究中评估候选基因多态性与侵袭性或慢性牙周炎易感性之间的关联。在日本人中，TNF-α基因5 '侧翼区的多态性与全身侵袭性牙周炎（G-AgP）的易感性无关，而VDR和IgG受体、FcγRIIIb、IL-1 RA基因VNTR（variable number of tandem repeats）与G-AgP易感性相关。TNFR 1和FcγRIIb基因的单核苷酸多态性（SNP）分别与G-AgP相关。CD 14基因的多态性与牙周炎的发生无关，但可能与牙周炎的早期活动有关，提示与骨稳态相关的多个基因作为组合因素与牙周炎的发生有关。本研究的结果可能有助于建立一个多遗传因素的牙周炎诊断系统。

项目成果

Toshiya Morozumi: "Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis."Journal of Periodontal Research. 36. 160-168 (2001)

Toshiya Morozumi：“用牙龈卟啉单胞菌脂多糖刺激的人中性粒细胞中超绒毛蛋白和血管内皮生长因子的 mRNA 表达升高。”牙周研究杂志。

Hideaki Tai: "Association of interleukin-1 receptor antagonist gene polymorphisms with early onset periodontitis in Japanese."Journal of Clinical Periodontology. 29. 882-888 (2002)

Hideaki Tai：“白介素 1 受体拮抗剂基因多态性与日本早发性牙周炎的关联。”临床牙周病学杂志。

T.Morozumi, T.Kubota, N.Sugita, Y.Ohsawa, K.Yamazaki, H.Yoshie: "Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from porphyromonas gingivalis."Journal of Periodontal

T.Morozumi、T.Kubota、N.Sugita、Y.Ohsawa、K.Yamazaki、H.Yoshie：“牙龈卟啉单胞菌脂多糖刺激的人中性粒细胞中超绒毛蛋白和血管内皮生长因子的 mRNA 表达升高。”牙周病杂志

Y.Shimada, H.Tai, M.Endo, T.Kobayashi, K.Yamazaki, H.Yoshie: "Analysis of tumor necrosis factor receptor 1 and 2 gene polymorphisms in Japanese patients with generalized aggressive periodontitis."Journal of Japanese Society of Periodontology. Vol.45. 267-

Y.Shimada、H.Tai、M.Endo、T.Kobayashi、K.Yamazaki、H.Yoshie：“日本广泛性侵袭性牙周炎患者肿瘤坏死因子受体 1 和 2 基因多态性分析。”日本牙周病学会杂志

Tetuso Kobayashi, et al.: "The Fcγ receptor genotype as a severity factor for chronic periodontitis in Japanese patients"Journal of Periodontology. 72・10. 1324-1331 (2001)

Tetuso Kobayashi 等：“Fcγ 受体基因型作为日本患者慢性牙周炎的严重性因素”，牙周病学杂志 72・10（2001 年）。