Gingival lymphocyte functions in dog and human. I. Blastogenic responses to mitogens II. Antibody formation

本研究的目的是检测犬炎症龈淋巴细胞的免疫球蛋白分泌情况，并与外周血淋巴细胞（PBL）和下颌下结淋巴细胞（SNL）的免疫球蛋白分泌情况进行比较。取5只牙菌斑性牙周炎犬的牙龈组织。采用胶原酶处理和ficoll -梯度法分离牙龈淋巴细胞、pbl和snl。淋巴细胞培养1 ~ 10天，用ELISA法测定培养上清的抗体浓度。实验结果如下：1)牙龈湿重1.5 g /只。恢复了4.60 x <10^6>（范围：2.5 - 6.8 x <10^6>）淋巴细胞，平均存活率为93%。2)与pbl和snl相比，牙龈淋巴细胞IgG的产生显著，而牙龈淋巴细胞IgG的产生低于pbl。在脉宽调制刺激下，牙龈淋巴细胞、pbl和snl的免疫球蛋白产量几乎相同。3)牙龈淋巴细胞成母活性低。这些结果表明，牙龈B淋巴细胞在体内受到高度刺激，并在从组织中恢复时积极分泌免疫球蛋白。

The purpose of this study was to examine the immunoglobulin production of lymphocytes from inflamed gingiva in dogs and compare with those of peripheral blood lymphocyte ( PBL ) and submandibular node lymphocyte ( SNL ). Gingival tissue was obtained from five dogs with plaque induced periodontitis. Gingival lymphocytes, PBLs and SNLs were isolated by collagenase treatment and Ficoll-gradient procedure . Antibody concentration of culture supernatants was estimated by ELISA during lymphocyte culture for one to ten days.The results were as follows; 1) Wet weight of gingiva was 1.5 gram per dog. A number of 4.60 x <10^6> ( range : 2.5 - 6.8 x <10^6> ) lymphocytes with a mean viability of 93 % was recovered. 2) IgG production of gingival lymphocytes was prominent as compared with those of PBLs and SNLs, while IgG production of gingival lymphocytes was less than that of PBLs. Immunoglobulin production of gingival lymphocytes, PBLs and SNLs was almost same level when stimulated with PWM. 3) The blastogenic activity of gingival lymphocytes was low.Those findings suggest that gingival B lymphocytes have been highly stimulated in vivo and had active secretion of immunoglobulin at the time of recovery from tissue.