Gingival lymphocyte functions in dog and human. I. Blastogenic responses to mitogens II. Antibody formation

本研究旨在检测犬牙龈炎淋巴细胞产生免疫球蛋白的能力，并与外周血淋巴细胞（PBL）和颌下淋巴结淋巴细胞（SNL）进行比较。牙龈组织取自5只患菌斑性牙周炎的狗。采用胶原酶处理和Ficoll梯度法分离牙龈淋巴细胞、PBL和SNLs。淋巴细胞培养1 ~ 10天，用ELISA法测定培养上清抗体浓度，结果如下：（1）牙龈湿重为1.5克/只;回收的淋巴细胞数量为4.60 x <10^6>（范围：2.5 - 6.8 x <10^6>），平均存活率为93%。2)牙龈淋巴细胞产生IgG的能力显著高于外周血淋巴细胞和SNL，但牙龈淋巴细胞产生IgG的能力低于外周血淋巴细胞。PWM刺激牙龈淋巴细胞、PBL和SNLs产生的免疫球蛋白水平基本一致。3)结果表明，牙龈B淋巴细胞在体内受到高度刺激，从组织中恢复后，其免疫球蛋白分泌活跃。

The purpose of this study was to examine the immunoglobulin production of lymphocytes from inflamed gingiva in dogs and compare with those of peripheral blood lymphocyte ( PBL ) and submandibular node lymphocyte ( SNL ). Gingival tissue was obtained from five dogs with plaque induced periodontitis. Gingival lymphocytes, PBLs and SNLs were isolated by collagenase treatment and Ficoll-gradient procedure . Antibody concentration of culture supernatants was estimated by ELISA during lymphocyte culture for one to ten days.The results were as follows; 1) Wet weight of gingiva was 1.5 gram per dog. A number of 4.60 x <10^6> ( range : 2.5 - 6.8 x <10^6> ) lymphocytes with a mean viability of 93 % was recovered. 2) IgG production of gingival lymphocytes was prominent as compared with those of PBLs and SNLs, while IgG production of gingival lymphocytes was less than that of PBLs. Immunoglobulin production of gingival lymphocytes, PBLs and SNLs was almost same level when stimulated with PWM. 3) The blastogenic activity of gingival lymphocytes was low.Those findings suggest that gingival B lymphocytes have been highly stimulated in vivo and had active secretion of immunoglobulin at the time of recovery from tissue.