Gingival lymphocyte functions in dog and human. I. Blastogenic responses to mitogens II. Antibody formation

牙龈淋巴细胞在狗和人类中发挥作用。

• 批准号: 60570892
• 负责人: YOSHIE Hiromasa
• 金额: $ 1.09万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570892/
• 关键词: Experimental periodontitis; Dog; Gingival lymphocyte; 抗体産生能

The purpose of this study was to examine the immunoglobulin production of lymphocytes from inflamed gingiva in dogs and compare with those of peripheral blood lymphocyte ( PBL ) and submandibular node lymphocyte ( SNL ). Gingival tissue was obtained from five dogs with plaque induced periodontitis. Gingival lymphocytes, PBLs and SNLs were isolated by collagenase treatment and Ficoll-gradient procedure . Antibody concentration of culture supernatants was estimated by ELISA during lymphocyte culture for one to ten days.The results were as follows; 1) Wet weight of gingiva was 1.5 gram per dog. A number of 4.60 x <10^6> ( range : 2.5 - 6.8 x <10^6> ) lymphocytes with a mean viability of 93 % was recovered. 2) IgG production of gingival lymphocytes was prominent as compared with those of PBLs and SNLs, while IgG production of gingival lymphocytes was less than that of PBLs. Immunoglobulin production of gingival lymphocytes, PBLs and SNLs was almost same level when stimulated with PWM. 3) The blastogenic activity of gingival lymphocytes was low.Those findings suggest that gingival B lymphocytes have been highly stimulated in vivo and had active secretion of immunoglobulin at the time of recovery from tissue.

本研究的目的是检测炎症牙周组织中淋巴细胞免疫球蛋白的产生，并与外周血淋巴细胞(PBL)和颌下结淋巴细胞(SNL)进行比较。牙周组织取自5只斑块性牙周炎犬。采用胶原酶处理和Ficoll梯度法分离牙龈淋巴细胞、PBL和SNL。用ELISA法测定淋巴细胞培养1~10天时培养上清液的抗体浓度，结果如下：1)牙龈湿重1.5g/只。共回收4.60x&lt；10^6；(范围：2.5-6.8x&lt；10^6&gt；)淋巴细胞，平均存活率为93%。2)牙周淋巴细胞的免疫球蛋白生成显著高于PBL和SNL，而牙周淋巴细胞的免疫球蛋白生成低于PBL。在脉宽调制刺激下，牙龈淋巴细胞、PBL和SNL产生的免疫球蛋白水平基本相同。3)牙周B淋巴细胞的分化活性较低，提示牙周B淋巴细胞在体内受到高度刺激，在组织恢复时免疫球蛋白分泌活跃。