Role of immunocytokine on the neuroendocrine sysytem

本研究从以下几个方面探讨了免疫细胞因子在神经内分泌系统中的作用。白细胞介素-1 β（IL-1 β）直接兴奋下丘脑视上神经元. IL-1 β通过洋地黄素增加心率、血压、体温和肾交感神经活动。此外，交感神经系统在IL-1 β诱导的反应中起重要作用. IL-1 β诱导室旁核去甲肾上腺素含量短暂下降，随后逐渐增加.近交系小鼠（STR/N）具有烦渴的特征，并在我们的实验中研究了中枢神经系统（CNS）在烦渴中的可能参与。此外，我们证明了STR/N小鼠中T细胞功能受损以及免疫系统和CNS之间的相互作用。在刘易斯大鼠佐剂性关节炎过程中，行为（体温、体力活动、进食和饮水行为）和血浆激素水平（血浆ACTH和皮质酮）在两个阶段同步变化：初级阶段（0 - 5天）和次级阶段（11 - 12天）。脾巨噬细胞产生IL-1，但血清IL-1活性在关节炎期间的任何一天都检测不到。c-fos蛋白在OVLT、MnPO、PVN、NTS和AP等脑区表达明显增强。

We studied the role of immunocytokine on the neuroendocrine sysytem by approaching as follows :1. Interleukin-1beta (IL-1beta) directly excited hypothalamic supraoptic neurons.2. IL-1beta increased the heart rate, blood pressure, body temperature and renal sympathetic nerve activity via prostaglandins. Moreover, sympathetic nervous system playd an important role in the IL-1beta induced response.3. IL-1beta induced a transient decrease followed by a gradual increase in noradrenalin content in the paraventricular nucleus.4. An inbred strain of mice (STR/N) is characterized by polydipsia and the possible involvement of the central nervous system (CNS) in the polydipsia has been studied in our experiments. Furthermore, we demonstrated impaired T cell functions and the interactions between the immune system and the CNS in STR/N mice.5. During adjuvant arthritis in Lewis rats, behavior (body temperature, physical activity, feeding and drinking behaviors) and plasma hormone levels (plasma ACTH and corticosterone) changed synchronously in two phases ; primary (day 0 - 5) and secondary phases (day 11 - ). Spleen macrophages produced IL-1, but serum IL-1 activity could not be detected on any days during the arthritis. Expression of c-fos protein was activated in the several brain regions including the OVLT,MnPO,PVN,NTS and AP.