Stress protein and fiber type

应激蛋白和纤维类型

• 批准号: 04454587
• 负责人: ATOMI Yoriko
• 金额: $ 4.03万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454587/
• 关键词: alphaB-crystallin; Stress protein; fiber type; tyroid holmone; 筋繊維組成; ラット後肢懸垂法; ストレッチ

The sequence of alphaB-crystallin has striking similarity to the suquence of small heat shock proteins (HSPs#) and increasing evidence indicates that it has also functional similarities with the small HSPs. The expression of alphaB-crystallin is induced upon stress. Similarly, amorphous alphaB-acrystallin aggregates are formed after ischemia. alphaB-crystallin can function as a molecular chaperone, by suppressing heat induced aggregation of other proteins, and by assisting refolding of denatured protein. These observations from in vitro experiments suggest that alphaB-crystallin has to be considered a member of the small HSPs. It seems reasonable to assume that the constitutive expression is required in soleus muscle because this muscle is continuously subjected to a more stressful environment, such as high temperature, oxidant injury, and higher rate of protein turnover, than other muscles and requires stress related functions provided by alphaB-crystallin. This drives us to the question whether the expression of alphaB-crystallin in stressful muscle is directly regulated with its high rate recruitment, which causes stressful environment, or indirectly regulated in a manner of expression as a fiber type specific protein. The relationship between the expression of alphaB-crystallin as stress protein and fiber-type were examined in three experiments of 1) those distributions in control rats, 2) relative changes due to muscle atropphy by hindlimb suspension, and 3) relative changes due to the treatment of thiroid hormone and PTU.For this purpose we used immuno-histochemical method to investigate these relationship using mouse monoclonal anti-myosin antibodies and rabbit anti-alphaB-crystallin polyclonal antibodies. In soleus muscles, type I fibers reacted much stronger with anti-alphaB-crystallin Ig, than type IIA fibers. In EDL muscles, type I fibers showed strong reactivity for anti-alphaB-crystallin Ig than type II fibers. In addition to the differences between ty

α B-晶状体蛋白的序列与小分子热休克蛋白（HSPs）序列具有惊人的相似性，越来越多的证据表明它与小分子热休克蛋白也具有功能相似性。α B-晶状体蛋白的表达在应激时被诱导。类似地，缺血后形成无定形α B-晶状体蛋白聚集体。α B-晶状体蛋白可以通过抑制热诱导的其它蛋白质的聚集和通过辅助变性蛋白质的重折叠而起到分子伴侣的作用。这些来自体外实验的观察结果表明，α B-晶状体蛋白必须被认为是小HSP的成员。这似乎是合理的假设，组成型表达所需的比目鱼肌，因为这种肌肉是连续受到更紧张的环境，如高温，氧化剂损伤，和更高的蛋白质周转率，比其他肌肉，并需要压力相关的功能提供的α B-晶体蛋白。这促使我们提出一个问题，即α B-晶体蛋白在应激肌肉中的表达是通过其高速率募集直接调节的，这导致应激环境，还是以纤维类型特异性蛋白的表达方式间接调节。作为应激蛋白的α B-晶状体蛋白的表达与纤维类型之间的关系在三个实验中进行了检查：1）对照大鼠中的那些分布，2）由于后肢悬吊引起的肌肉萎缩的相对变化，应用免疫组化方法，用鼠抗人甲状腺素单克隆抗体（McAb）检测甲状腺素与PTU治疗后的变化。肌球蛋白抗体和兔抗α B-晶状体蛋白多克隆抗体。在比目鱼肌中，I型纤维与抗α B-晶体蛋白IG的反应比IIA型纤维强得多。在趾长伸肌中，I型纤维比II型纤维对抗α B-晶体蛋白IG表现出更强的反应性。除此之外，

项目成果

Arai, H., M.Yamasita and Y.Atomi: "Response of Cultured Cell to stretch stimulation" Morphology and orientation Proc.Dept.Sports Sci.Univ.Tokyo. 28(in printing). (1994)

Arai, H.、M.Yamasita 和 Y.Atomi：“培养细胞对拉伸刺激的反应”形态学和方向 Proc.Dept.Sports Sci.Univ.Tokyo。

Yoriko,Atomi: "Decreased αB-crystallin in soleus muscle atrophy and role of αB-crystallin in muscle" In integration of Medical and Sport Sciences(Edited by Sato et al). 171-192 (1992)

Yoriko, Atomi：“比目鱼肌萎缩中 αB-晶状体蛋白的减少以及 αB-晶状体蛋白在肌肉中的作用”《医学与运动科学整合》（Sato 等人编辑）171-192 (1992)。

跡見順子: "可塑性の鍵を握るプロテアーゼ" 運動生化学. (印刷中). (1994)

Junko Atomi：“蛋白酶是可塑性的关键”《运动生物化学》（出版中）。

Atomi,Y.,et al.: "Early Changes of αB-crystallin mRNA to mechanical tension and innervatin in rat skeletal muscle." Biochem.Biophys.Res.Commun.181. 1323-1330 (1991)

Atomi, Y. 等人：“大鼠骨骼肌中 αB-晶状体蛋白 mRNA 的早期变化”，Biochem.Biophys.Res.Commun.181（1991）。

Atomi,Y.(Edited by Sato,Y.,J.Poortsman,I.Hashimoto,): "Integration of Medical and Sports Sciences." Karger,446 (1992)

Atomi,Y.（由 Sato,Y.、J.Poortsman、I.Hashimoto 编辑）：“医学与运动科学的整合”。