Mechanism of molecular basis for "Tailor-made and measurable physical exercise program "and the educational application, evaluated from stress protein (molecular chaperone).

从细胞骨架和细胞外基质（ECM）的双重蛋白质纤维结构及其分子伴侣（应激蛋白）的角度，研究了“量身定制的可测量的体育锻炼计划”的原理，并对产生运动的主要组织肌肉和关节及其构成细胞进行了研究。重要结果如下：1) B-crystallin (B)的c端crytallin结构域作为小管蛋白聚集的伴侣。2)由于GFP-1 B在培养的跳动心脏细胞中呈条纹状，它一定是作为结构蛋白的伴侣蛋白。3) OB的N端区对map -微管的解聚起抑制作用。4)废用性肌肉萎缩导致内质网特异性胶原伴侣蛋白HSP47降低。在培养的成肌细胞中也得到了类似的结果。在HSP47基因的调控域中可能发现新的重力响应元件。5)培养肌肉细胞温度升高，有利于肌肉分化，增加慢肌球蛋白表达。6)轻度跑步运动诱导大鼠膝关节III型胶原、透明质酸合酶，意味着“好运动”的存在，有助于机体细胞环境向年轻化转变。7)久坐老年人骨骼肌中磷酸化的CB/HSP27和泛素化蛋白增加，表明蛋白质降解降低。8) HSP70与HPA轴外周组织肾上腺中糖皮质激素合成的关键酶StAR共沉淀。应激蛋白是连接细胞和机体的良好、可测量的适应性标志。从2006年开始，东京大学在体育与健康科学一年级的必修课程中引入了“运动引起细胞变化”的学习课程。学生的问卷调查结果表明，这种知识学习与亲身实验相结合的教育方式的有效性有所贡献。

The principle of "tailor-made and measurable physical exercise program' has been studied from dual protein fiber structures of the cytoskeleton and extracellular matrix (ECM), and their molecular chaperone (stress proteins) for main tissues creating exercise, muscles and joints, and their constituting cells. Important results are as follows; 1) The C-terminal located crytallin domain in B-crystallin (B) works as chaperone against tubulin aggregation. 2) As GFP-1 B in beating cultured heart cells shows striation, it must function as chaperone for structural proteins. 3) The N terminal region of OB plays to decrease depolymerization of MAP-microtubule. 4) Disuse muscle atrophy induces the decrease of specific collagen chaperone HSP47 in ER. Similar results were obtained in cultured myoblasts. Possible new gravity-response element in the regulatory domain of HSP47 gene will be identified. 5) The increased temperature of 2 11 of cultured muscle cells facilitates muscle differentiation and increase slow myosin expression. 6) Mild running exercise induces type III collagen, hyaluronic acid synthase in rat knee joint, meaning existence of "good exercise" to contribute to change toward younger cellular environment in our body. 7) Phosphorylated CB/HSP27 and ubiquitinated proteins in human sedentary elderly skeletal muscle increase, suggesting lowered protein degradation. 8) HSP70 was coprecipitated with a key enzyme of glucocorticoid synthesis: StAR in adrenal gland, the peripheral tissue of HPA axis. Stress protein is available for a marker of good and measurable, adaptation connecting both cells and body. 1 earning program of cellular changes by exercise was introduced into compulsory curriculum of physical education and health science for 1st year students the University of Tokyo from 2006. Results of questionnaires by students indicated a contribution of the effectiveness of such a mixed education of knowledge learning and experiment using own body.