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Mechanism of molecular basis for "Tailor-made and measurable physical exercise program "and the educational application, evaluated from stress protein (molecular chaperone).

“量身定制、可测量的体育锻炼计划”的分子基础机制和教育应用，从应激蛋白（分子伴侣）进行评估。

基本信息

批准号：
17200039
负责人：
ATOMI Yoriko
金额：
$ 30.95万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

The principle of "tailor-made and measurable physical exercise program' has been studied from dual protein fiber structures of the cytoskeleton and extracellular matrix (ECM), and their molecular chaperone (stress proteins) for main tissues creating exercise, muscles and joints, and their constituting cells. Important results are as follows; 1) The C-terminal located crytallin domain in B-crystallin (B) works as chaperone against tubulin aggregation. 2) As GFP-1 B in beating cultured heart cells shows striation, it must function as chaperone for structural proteins. 3) The N terminal region of OB plays to decrease depolymerization of MAP-microtubule. 4) Disuse muscle atrophy induces the decrease of specific collagen chaperone HSP47 in ER. Similar results were obtained in cultured myoblasts. Possible new gravity-response element in the regulatory domain of HSP47 gene will be identified. 5) The increased temperature of 2 11 of cultured muscle cells facilitates muscle differentiation and increase slow myosin expression. 6) Mild running exercise induces type III collagen, hyaluronic acid synthase in rat knee joint, meaning existence of "good exercise" to contribute to change toward younger cellular environment in our body. 7) Phosphorylated CB/HSP27 and ubiquitinated proteins in human sedentary elderly skeletal muscle increase, suggesting lowered protein degradation. 8) HSP70 was coprecipitated with a key enzyme of glucocorticoid synthesis: StAR in adrenal gland, the peripheral tissue of HPA axis. Stress protein is available for a marker of good and measurable, adaptation connecting both cells and body. 1 earning program of cellular changes by exercise was introduced into compulsory curriculum of physical education and health science for 1st year students the University of Tokyo from 2006. Results of questionnaires by students indicated a contribution of the effectiveness of such a mixed education of knowledge learning and experiment using own body.

从细胞骨架和细胞外基质（ECM）的双重蛋白质纤维结构及其分子伴侣（应激蛋白）研究了“量身定制和可测量的体育锻炼计划”的原理，这些蛋白质纤维结构用于产生运动的主要组织、肌肉和关节及其构成细胞。主要结果如下：1）B-晶体蛋白（B）的C-末端crytallin结构域作为分子伴侣抑制微管蛋白的聚集。2)由于GFP-1 B在跳动的培养心脏细胞中显示条纹，它必须作为结构蛋白的伴侣蛋白。3)OB的N端区域起着减少微管解聚的作用。4)废用性肌萎缩导致内质网中特异性胶原伴侣HSP 47表达减少。在培养的成肌细胞中获得了类似的结果。HSP 47基因调控域中可能存在新的重力反应元件。5)2 - 11培养的肌肉细胞的温度升高，促进肌肉分化，并增加缓慢肌球蛋白的表达。6)温和的跑步运动诱导大鼠膝关节中的III型胶原蛋白、透明质酸合成酶，这意味着“良好运动”的存在有助于我们体内细胞环境向年轻化的方向变化。7)人久坐老年骨骼肌中磷酸化CB/HSP 27和泛素化蛋白增加，表明蛋白质降解降低。8)HSP 70与糖皮质激素合成的关键酶星星在HPA轴的外周组织肾上腺中共沉淀。应激蛋白是一种良好的、可测量的、连接细胞和身体的适应性标志物。从2006年起，东京大学将运动引起的细胞变化的1个学习计划引入体育和健康科学一年级学生的必修课程。学生的问卷调查结果表明，这种知识学习和实验使用自己的身体混合教育的有效性的贡献。