The evaluation of physical exercise by stress protein.

应激蛋白对体育锻炼的评价

• 批准号: 08458016
• 负责人: ATOMI Yoriko
• 金额: $ 4.93万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458016/
• 关键词: stress protein; exercise; HSP70; in situ hybridization; in situ DNA hybridization; HSF; HSE; ゲルシフト

The physiological and biochemical evaluation of the physical exercise is not clear. Physical exercises induces various stimuli depending on exercise intensity and duration. Recently stress protein, which is induced by the various stress, is well studied in the field of basic and medical sciences. In the study, we examined the propriety of stree protein for the physical exercise.We did experiments using rats on treadmill running. The induction of stress proteins was investigated at the level of DNA and RNA expression, using in situ DNA hybridization and in situ hybridization. In addition, blood lactate and blood glucose concentrations after exercise were measured. Since the blood lactate concentration increased to high level and the blood glucose concentration decreased to lower level after exercise, the exercise intensity used in this study was rather high. As a result, we found that the binding of HSE-HSF was increased in rat kidney after exercise with in situ DNA hybridization, compared with control group. The induction of HSP70mRNA with in situ hybridization after exercise increased in rat a dernal, spleen, kidney, liver and heart compared with control group. However the induction of HSP70mRNA didn's increase in rat skeletal muscle (soleus, plantaris, EDL). From these results, the expression of stress protein seems to be available for the biochemical evaluation of physical exercise. In addition, in situ hybridization is an effective method for the detection of HSP induction by exercise.

体育锻炼的生理生化评价尚不明确。根据运动强度和持续时间的不同，体育锻炼会产生不同的刺激。应激蛋白是由各种应激诱导产生的一种蛋白质，近年来在基础科学和医学领域得到了广泛的研究。在这项研究中，我们考察了树蛋白在体育锻炼中的适用性。我们用老鼠在跑步机上跑步做实验。采用原位DNA杂交和原位杂交技术，在DNA和RNA表达水平上研究应激蛋白的诱导。此外，还测量了运动后的血乳酸和血糖浓度。由于运动后血乳酸浓度升高到较高水平，血糖浓度下降到较低水平，因此本研究采用的运动强度较高。结果，我们通过原位DNA杂交发现，与对照组相比，运动后大鼠肾脏中HSE-HSF的结合增加。与对照组相比，运动后大鼠真皮、脾脏、肾脏、肝脏和心脏的HSP70mRNA诱导量增加。而HSP70mRNA在大鼠骨骼肌（比目鱼肌、足底肌、EDL肌）中的表达没有增加。从这些结果来看，应激蛋白的表达似乎可以用于体育锻炼的生化评价。此外，原位杂交是检测运动诱导热休克蛋白的有效方法。