The evaluation of physical exercise by stress protein.

对体育锻炼的生理生化评价尚不明确。体育锻炼根据锻炼强度和持续时间的不同，会产生不同的刺激。近年来，由各种应激诱导的应激蛋白在基础科学和医学领域得到了广泛的研究。在这项研究中，我们考察了应激蛋白对体育锻炼的适宜性。我们用大鼠在跑步机上跑步进行了实验。利用原位DNA杂交和原位杂交技术，从DNA和RNA表达水平研究了胁迫蛋白的诱导作用。此外，还测量了运动后的血乳酸和血糖浓度。由于运动后血乳酸浓度升高到较高水平，血糖浓度下降到较低水平，所以本研究采用的运动强度较大。结果发现，与对照组相比，运动后大鼠肾脏HSE-HSF的结合量增加。与对照组相比，运动后大鼠皮肤、脾、肾、肝、心等脏器中HSP70mRNA的表达明显增加。而大鼠骨骼肌(比目鱼肌、足底肌、足底肌)中HSP70mRNA的表达无明显增加。从这些结果来看，应激蛋白的表达似乎可以用于体育锻炼的生化评价。此外，原位杂交是检测运动性热休克蛋白诱导的有效方法。

The physiological and biochemical evaluation of the physical exercise is not clear. Physical exercises induces various stimuli depending on exercise intensity and duration. Recently stress protein, which is induced by the various stress, is well studied in the field of basic and medical sciences. In the study, we examined the propriety of stree protein for the physical exercise.We did experiments using rats on treadmill running. The induction of stress proteins was investigated at the level of DNA and RNA expression, using in situ DNA hybridization and in situ hybridization. In addition, blood lactate and blood glucose concentrations after exercise were measured. Since the blood lactate concentration increased to high level and the blood glucose concentration decreased to lower level after exercise, the exercise intensity used in this study was rather high. As a result, we found that the binding of HSE-HSF was increased in rat kidney after exercise with in situ DNA hybridization, compared with control group. The induction of HSP70mRNA with in situ hybridization after exercise increased in rat a dernal, spleen, kidney, liver and heart compared with control group. However the induction of HSP70mRNA didn's increase in rat skeletal muscle (soleus, plantaris, EDL). From these results, the expression of stress protein seems to be available for the biochemical evaluation of physical exercise. In addition, in situ hybridization is an effective method for the detection of HSP induction by exercise.