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Evaluation of Physical Exercise from Stress Protein (2)-Evallluation of Hypoactivity and Training Effect-

从应激蛋白评价体育锻炼（二）-活动量减少及训练效果的评价-

基本信息

批准号：
10480004
负责人：
ATOMI Yoriko
金额：
$ 7.42万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10480004/
关键词：
stress protein running exercise liver skeletal muscle HSP70 hindlimb suspension model muscle atrophy GPT 不活動 αB-クリスタン HSP72 HSC73 後肢懸垂運動

项目摘要

In this study, 1) the effects of running exercise on the expression of stress protein, and 2) the effects of hindlimb suspension on the cytoskeleton tubulin/microtubule system which is the substrate for stress protein, were examined. 1) The stress protein expressions of HSP72 and HSC73, inducible and constitutive forms of HSP7Os, in exercise relating tissues (skeletal muscles and heart) and exercise non-relating tissues after two levels of exercises of low (speed : 13 m/min ; blood lactate<4 mM : LIE) and high (24 m/min ; blood lactate > 4 mM : HIE) intensities of running exercise were measured by Western blotting using anti-HSP72 and HSC73 antibodies. Serum GTP was measured as an index of liver damage after exercise. The HSP expressions after heat shock were measured as positive controls. Both the GPT levels and inducible HSP72 (3〜4 times) increased after HIE, as well as with small increases of HSC73 (20%), while only HSC73 increase was observed after LIE.Both HSP70s depressed after both exercises in hearts. 2) Tubulin content in the 5-day-suspended soleus muscles was significantly and specifically decreased, while that in the stretched muscles increased 3 times at 5 days and over 3 times at the 10 days of suspension. αB-crysallin content in precipitate fraction of muscle protein increased in suspended soleus muscles, suggesting that the association of αB-crystallin with denatured proteins in precipitate fraction increased in suspended muscle. From these results, habitual physical activity and low intensity of exercise express constitutive expressed form of HSP70 (HSC73) and higher intensity of exercise expresses inducible form of HSP70 (HSP72).

本研究考察了跑步运动对应激蛋白表达的影响，以及后肢悬浮对应激蛋白底物细胞骨架微管/微管系统的影响。1)采用抗HSP72和HSC73抗体，采用Western blotting检测低（速度：13 m/min；血乳酸<4 mM: LIE）和高（24 m/min；血乳酸<4 mM: HIE）两种强度跑步运动后运动相关组织（骨骼肌和心脏）和运动非相关组织中HSP72和HSC73的应激蛋白表达。运动后测定血清GTP作为肝损伤指标。以热休克后HSP表达为阳性对照。HIE后GPT水平和诱导HSP72水平均升高（3 ~ 4倍），HSC73水平小幅升高（20%），LIE后HSC73水平仅升高。两种运动后心脏hsp70均下降。2)悬吊5 d比目鱼肌微管蛋白含量显著特异性降低，而伸展肌微管蛋白含量在悬吊5 d时增加3倍，在悬吊10 d时增加3倍以上。悬浮比目鱼肌中肌蛋白沉淀部分α b -结晶蛋白含量增加，表明悬浮比目鱼肌中α b -结晶蛋白与沉淀部分变性蛋白的关联增加。由此可见，习惯体育活动和低强度运动表达HSP70组成型表达（HSC73），高强度运动表达HSP70诱导型表达（HSP72）。