Evaluation of Physical Exercise from Stress Protein (2)-Evallluation of Hypoactivity and Training Effect-

本研究探讨了跑步运动对应激蛋白表达的影响，以及后肢悬吊对应激蛋白底物细胞骨架微管蛋白/微管系统的影响。1)HSP 70的诱导型和组成型HSP 72和HSC 73在运动相关组织中的应激蛋白表达（骨骼肌和心脏）和运动非相关组织后，两个水平的运动低（速度：13 m/min ;血乳酸<4 mM：LIE）和高使用抗HSP 72和HSC 73抗体通过蛋白质印迹法测量跑步运动（24 m/min ;血乳酸> 4 mM：HIE）的强度。测定血清GTP作为运动后肝损伤的指标。以热休克后的HSP表达作为阳性对照。HIE后GPT和诱导型HSP 72（3 ~ 4倍）均升高，HSC 73（20%）略有升高，而LIE后仅HSC 73升高，两种运动均使心脏HSP 70降低。2)悬吊5 d比目鱼肌微管蛋白含量明显降低，而拉伸5 d时增加3倍，悬吊10 d时增加3倍以上。在悬浮状态下，肌肉蛋白沉淀组分中α B-晶体蛋白含量增加，表明α B-晶体蛋白与沉淀组分中变性蛋白的结合增加。从这些结果来看，习惯性体力活动和低强度运动表达组成型表达形式的HSP 70（HSC 73），而较高强度运动表达诱导型HSP 70（HSP 72）。

In this study, 1) the effects of running exercise on the expression of stress protein, and 2) the effects of hindlimb suspension on the cytoskeleton tubulin/microtubule system which is the substrate for stress protein, were examined. 1) The stress protein expressions of HSP72 and HSC73, inducible and constitutive forms of HSP7Os, in exercise relating tissues (skeletal muscles and heart) and exercise non-relating tissues after two levels of exercises of low (speed : 13 m/min ; blood lactate<4 mM : LIE) and high (24 m/min ; blood lactate > 4 mM : HIE) intensities of running exercise were measured by Western blotting using anti-HSP72 and HSC73 antibodies. Serum GTP was measured as an index of liver damage after exercise. The HSP expressions after heat shock were measured as positive controls. Both the GPT levels and inducible HSP72 (3〜4 times) increased after HIE, as well as with small increases of HSC73 (20%), while only HSC73 increase was observed after LIE.Both HSP70s depressed after both exercises in hearts. 2) Tubulin content in the 5-day-suspended soleus muscles was significantly and specifically decreased, while that in the stretched muscles increased 3 times at 5 days and over 3 times at the 10 days of suspension. αB-crysallin content in precipitate fraction of muscle protein increased in suspended soleus muscles, suggesting that the association of αB-crystallin with denatured proteins in precipitate fraction increased in suspended muscle. From these results, habitual physical activity and low intensity of exercise express constitutive expressed form of HSP70 (HSC73) and higher intensity of exercise expresses inducible form of HSP70 (HSP72).