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Evaluation of physical exercise on the basis of stress protein (3) - Relation to mechanical and physical stress induced in our body and the effect of constitutive expression and over expression -

基于应激蛋白的体育锻炼评价(3) - 与体内诱发的机械和物理应激的关系以及组成型表达和过度表达的影响 -

基本信息

批准号：
12308001
负责人：
ATOMI Yoriko
金额：
$ 28.5万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

In the present study new methods to study the effect of stress protein on adaptability and were developed and three different results were obtained. 1) In the hindlimb suspension model, cytoskeletal protein, tubulin also decreased as well as αB-crystallin. αB-crystallin has chaperone function for tubulin as previously reported (Arai & Atomi, 1997). Muscle shortening suppresses the expression of αB-crystallin. This might facilitate tubulin denaturing as substrate of αB-crystallin. 2) The suppression of αB-crystallin by way of antisense method in cultured cells, induced the change of cell shape and weakened cell adhesion, resulting in inducing abnormal motility using time-lapse analysis. The cell that over expresses αB-crystallin widely develops, strongly adheres. It is shown that αB-crystallin is essential for tonic cell contraction and adhesion. 3) New method to study the responses of mice (C57B6) to exercise was developed for the inbred mice that genetically identified. Anaerobic Threshold (AT) was measured by the use of VO_2 VCO_2 and identified to be me same as LT by the analysis of blood lactate concentration. Interestingly Hsp72 expressions was significantly increased after 30' treadmill running exercise only at lower intensity than LT level, but not higher than LT. This phenomenon is similar to stress tolerance, which is obtained after mild stress previously reported.

本研究开发了研究胁迫蛋白对植物适应性影响的新方法，得到了三种不同的结果。1)在后肢悬吊模型中，细胞骨架蛋白、微管蛋白和αB-晶状体蛋白也降低。正如先前报道的那样，αB-晶体蛋白具有微管蛋白的伴侣功能(Arai&Thomas，1997年)。肌肉缩短抑制αB-晶状体蛋白的表达。这可能有助于作为αB-晶体蛋白底物的微管蛋白的变性。2)通过反义方法抑制培养细胞中αB-晶状体蛋白的表达，导致细胞形态改变，细胞黏附减弱，延时分析显示细胞运动异常。过度表达αB-晶体蛋白的细胞广泛发育，黏附能力强。结果表明，αB-晶状体蛋白对紧张性细胞的收缩和黏附是必需的。3)对经过遗传鉴定的近交系小鼠(C57B6)，建立了研究其运动反应的新方法。用VO_2、VCO_2测定无氧阈值(AT)，经血乳酸浓度分析确定与LT相同。有趣的是，30‘跑台运动后HSP72的表达仅在低于LT的强度时显著增加，但不高于LT。这种现象类似于先前报道的在轻度压力后获得的压力耐受性。