Alteration of metabolism and biotransformation of environmental chemicals in the liver cells due to changes in the intracellular level

由于细胞内水平的变化而改变肝细胞内环境化学物质的代谢和生物转化

• 批准号: 05454224
• 负责人: TAKANO Takehito
• 金额: $ 4.29万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454224/
• 关键词: Hypoxia; Antioxidant; Liver cell; Carbon tetrachloride; alpha-tocopherol; Ferric Chloride; Circadian rhythm; Food; 複合影響; 酸素分圧; 一酸化炭素; 四塩化炭素

The combined effects of CO hypoxia and antioxidants in the food on the CC14-induced liver injury were studied in the rat. The CO hypoxia enhanced the CC14-induced liver injury, whereas alpha-tocopherol and ferric chloride had a protective effect on the CC14-induced liver injury. The degree of CC14-induced liver injury was dependent upon the time of administration of CC14, and the administration during light phase produced higher toxicities. The expression of c-Ha-ras producing proteins in the liver cells were also detected by immunohistochemical procedures, showing that the expression of c-Ha-ras positive proteins were greater in the light phase that in the dark phase. The effects of alpha-tocopherol on the liver injury was greater in the light phase than in the dark phase. The same tendency was observed with regard to ferric chloride. These results indicated that alpha-tocopherol and ferric chloride, food additives, had protective effects on liver injury, and this protective effects were dependent upon the time of administration of these substances.

研究了CO缺氧和食物中抗氧化剂对cc14所致大鼠肝损伤的联合作用。CO缺氧对cc14诱导的肝损伤有增强作用，而α -生育酚和氯化铁对cc14诱导的肝损伤有保护作用。CC14引起的肝损伤程度与给药时间有关，且轻期给药产生较高的毒性。通过免疫组织化学方法检测肝细胞中c-Ha-ras生成蛋白的表达，显示c-Ha-ras阳性蛋白在光照期的表达高于黑暗期。α -生育酚对肝损伤的影响在光期大于暗期。在氯化铁方面也观察到同样的趋势。结果表明，食品添加剂-生育酚和氯化铁对肝损伤具有保护作用，且这种保护作用与给药时间有关。

项目成果

Kobayashi Y., Motohashi Y., Miyazaki Y., Kishi K., and Takano T.: "Changes in immunohistochemical distribution of cytochrome MC-P-448 in the rat liver during acclimation to cold." Int.J.Biometeor.37. 207-211 (1993)

Kobayashi Y.、Motohashi Y.、Miyazaki Y.、Kishi K. 和 Takano T.：“在适应寒冷过程中，大鼠肝脏中细胞色素 MC-P-448 的免疫组织化学分布发生变化。”

Liu J., Miyakawa H., Liu J., Mori K., Takano T., and Marumo F., and Sato C.: "Characterization of glutathione efflux from Hep G2 cells. Res.Communn" Mol.Pathol.Pharmacol.85. 261-270 (1994)

Liu J.、Miyakawa H.、Liu J.、Mori K.、Takano T.、Marumo F. 和 Sato C.：“Hep G2 细胞谷胱甘肽流出的表征。Res.Communn”Mol.Pathol.Pharmacol。

Sripaung N etal.: "Simultaneous administration of ethanal on sturene metabolism under fed and fasted conditicns in the isalated perfused liuer" J.Toxicol.Enviran.Health. (印刷中). (1995)

Sripaung N 等人：“在隔离灌注流中同时施用乙醛对进食和禁食条件下的苯乙烯代谢”J.Toxicol.Enviran.Health（出版中）。

Liu J., Sato C., Takano T., and Marumo F.: "Characterization of vasopressin-mediated GSH efflux from Hep G2 cells : Significance of protein kinase C." Lifr Sci.52. 1217-1223 (1993)

Liu J.、Sato C.、Takano T. 和 Marumo F.：“Hep G2 细胞中加压素介导的 GSH 流出的表征：蛋白激酶 C 的意义。”

Sripaung N., Motohashi Y., Nakamura K., and Takano T.: "Effects of nutritional conditions on the metabolic interaction of ethanol and stirene in the perfused rat liver" Jpn.J.Hyg.49,1. 157 (1994)

Sripaung N.、Motohashi Y.、Nakamura K. 和 Takano T.：“营养条件对灌注大鼠肝脏中乙醇和苯乙烯代谢相互作用的影响”Jpn.J.Hyg.49,1。