The Effects of Inhalational Anesthetics on Cancer Cells-Carcinostatic Effects of Inhalational Anesthetics-

已知吸入麻醉剂对免疫系统和几种其他类型的细胞具有抑制作用。本研究以体外培养的大鼠肝癌细胞为实验材料，在3 ~ 5 MAC浓度下，于CO2培养箱中培养24 h，观察不同浓度的挥发性麻醉药异氟醚、七氟醚、安氟醚和氟烷对肝癌细胞的作用。接触组和非接触对照组之间没有显著差异，但接触组的存活率略有下降。不同挥发性麻醉药对肝癌细胞存活率的影响差异无统计学意义。大鼠通过股静脉接受全胃肠外营养，并进行气管造口术以进行人工呼吸。吸入麻醉药1 MAC，连续5天，对照组用Barbi吸入麻醉 ...更多信息 图拉泰。两组均给予肌松药。两组在麻醉给药前后测定肿瘤直径，比较变化率。对照组和各挥发性麻醉药组之间的发生率无显著差异。同样，接受不同挥发性麻醉剂的大鼠之间的比率也没有显著差异。在这项研究中，无论是在体外还是在体内，挥发性麻醉剂都倾向于抑制癌细胞，但没有达到统计学显著的程度。未能获得良好结果的原因被认为是：在培养实验中，我们没有使用超过临床情况下使用的挥发性麻醉剂浓度;在活体大鼠实验中，麻醉剂浓度低，吸入时间短。人类能够比大鼠耐受更高水平的这些麻醉剂，并且可以接受更广泛的治疗形式，例如使用面罩重复麻醉。通过与使用线性偏振近红外线的热疗或利用内毒素诱导的肿瘤坏死因子的疗法结合使用，可以预期更大的有效性。我们计划研究这些联合疗法。少

Inhalation anesthetics are known to have a suppressive effect on the immune system and several other types of cell. In this study, we investigated the action of volatile anesthetics isoflurane, sevoflurane, enflurane and halothane on rat hepatic cancer cells in vitro and in vivo.Hepatic cancer cell cultures were exposed to volatile anesthetics at three to five MAC for 24 hours in tissue culture boxes placed in a CO2 incubator. There was no significant difference between the exposed and non-exposed-control groups, although viability was slightly reduced in the exposed group. No statistically significant difference in viability was observed after exposure to different volatile anesthetics.Hepatic cancer cell were subcutaneously placed in rats to form tumors. Rats received total parenteral nutrition via the femoral vein and were subjected to tracheostomy to enable artificial respiration. One MAC of volatile anesthetics was **en for five days ; the control group was anesthetized with barbi … More turate. Both groups received muscular relaxant. The tumor diameter was determined before and after anesthetic administration in both groups to compare the rate of change. No significant differences were observed in rate between the control and each volatile anesthetic group. Similarly, the rates were not significantly different among rats receiving different volatile anesthetics.In this study, both in vitro and in vivo, volatile anesthetics tended to suppress cancer cells, but not to a statistically significant extent. The reasons for failing to obtain good results are considered to be : in the culture experiment we did not use concentration of volatile anesthetics exceeding those used in clinical situations ; in living rat experiments the anesthetic concentrations were low and inhalation time was short. Humans are able to tolerate much higher levels of these anesthetics than rat and can undergo a wider range of forms of treatment such as repeated anesthesia using a mask. Greater effectiveness can be expected by using them in combination with thermotherapy using linear polarized near infrared or therapies utilizing tumor necrosis factor induced by endotoxins. We plan to investigate these combined therapies. Less