The study of virus DNA polymorphisms and its application to paternity testing

病毒DNA多态性研究及其在亲子鉴定中的应用

基本信息

批准号：
05670400
负责人：
KOBAYASHI Ryo
金额：
$ 1.34万
依托单位：
Juntento University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670400/
关键词：
personal identification paternity testing Epstein-Barr virus short tandem repeat DNA PCR polymorphism polymorphism 扁桃腺 in situ hybridization

项目摘要

In this project, we have tried to find the DNA markers that might be acquired in persons after their birth and be named acquired DNA markers. We have also tried to apply them for personal identification and paternity testing. In 1993, the third complementarity-determining region (CDR3) of immunoglobulin heavy chain gene was selected as one of acquired DNA markers, because this gene was rearranged during B-cell differentiation. However, the life span of the mature B-cells whose immunoglobulin gene was rearranged is generally considered to be about 2 weeks. Therefore, we did not think that this gene was the acquired DNA marker that could identify individuals. In 1994, we selected virus genes, especially Epstein-Barr (EB) virus as the acquired DNA marker, because this virus is a human herpesvirus that is carried by more than 90% of human population worldwide. We could detect the EB virus DNA in 92% of 98 DNA samples from peripheral blood lymphocytes using PCR that amplified BamW region of EB virus. EB virus DNA might be the acquired DNA marker, because this virus infects persons after their birth, and could be easily differentiable from the host DNA.In 1995, we focused the polymorphism of EB virus. EB virus has several short tandem repeat sequences and it is known that part of these sequences sometimes mutate during asymptomatic infection. So, we amplified the region of short tandem repeats of EB virus in the DNA isolated from peripheral blood lymphocytes using PCR and could observe the polymorphisms of EB virus in individuals. We concluded that EB virus might be considered as one of acquired DNA markers. However, we don't know how many strains of EB virus there are in persons, or how long EB virus is infected. These subjects will be had to elucidated.

在这个项目中，我们试图找到可能在其出生后人获得的DNA标记，并被命名为获得的DNA标记。我们还试图将它们应用于个人识别和亲子鉴定。 1993年，免疫球蛋白重链基因的第三个互补性区域（CDR3）被选为获得的DNA标记之一，因为该基因在B细胞分化过程中被重新排列。然而，通常认为重新排列免疫球蛋白基因的成熟B细胞的寿命约为2周。因此，我们认为该基因是可以识别个体的获得的DNA标记。在1994年，我们选择了病毒基因，尤其是爱泼斯坦 - 巴尔（EB）病毒作为获得的DNA标记，因为该病毒是一种人类疱疹病毒，全球超过90％的人口携带。我们可以使用放大EB病毒的BAMW区域的PCR中的98个DNA样品中92％的EB病毒DNA。 EB病毒DNA可能是获得后的DNA标记，因为该病毒在出生后会感染患者，并且很容易与宿主DNA差异化。在1995年，我们集中了EB病毒的多态性。 EB病毒具有几个短的串联重复序列，众所周知，这些序列的一部分有时在无症状感染过程中突变。因此，我们使用PCR从外周血淋巴细胞中分离出的DNA中EB病毒的短串联重复区域扩增了区域，并且可以观察到个体中EB病毒的多态性。我们得出的结论是，EB病毒可能被视为获得的DNA标记之一。但是，我们不知道有多少人患有eB病毒，或者EB病毒被感染了多长时间。这些受试者将不得不阐明。