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Human cytomegalovirus infection and anti-receptor antibody

人巨细胞病毒感染与抗受体抗体

基本信息

批准号：
05670700
负责人：
ABE Toshiaki
金额：
$ 1.22万
依托单位：
Teikyo University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670700/
关键词：
cytomegalovirus HNK-1 sulphated glycosphingolipids anti-receptor antibody SGPG SGLPG 硫酸化糖鎖抗体

项目摘要

In the present study we found that sera from patients with congenital CMV infection showed elevated titers* antibodies against sulfated glucuronyl glycosphingolipids (SGGL), which contain a monoclonal antibody HN* 1 reactive carbohydrate epitope. SGPG contains sulfated glucuronic acid on its non-reducing end of nLc4* and SGLPG has a similar structure but with an additional unit of lactosamine. Interesingly the antibodies* CMV infected patients'sera showed a higher reactivity to SGLPG than SGPG.This unique specificity * different from that of other HNK-1 related antibodies. Furthermore both anti-sulfatide and anti-SG* antibodies were absorbed with sulfatide-conjugated octyl-Sepharose column and heparin-Sepharose colur* whereas CMV specific IgG titer was not decreased by the absorption of anti-sulfated GL antibody. Th* results suggested that CMV infection might specifically induce production of antibodies against sulfated (* whereas these antibodies differed from CMV specific antibody.Then we studied whether the antibodies were produced against altered glycoconjugate patterns of host cell* a result of CMV infection. It was found that purified HNK-1 antibody bound to HCMV-positive and -negat* antigens. Anti-SGGL antibodies, which were partially purified from patient's sera by affinity chromatograp* also bound to both antigens, whereas the reactivity was lower than that of anti-HCMV antibodies. These res* indicate that a level of the HNK-1 epitope expression was not altered by HCMV infection and also t* glycoconjugates containing HNK-1 epitope were present in HCMV permissive cells. Furthermore, * pretreatment of the purified HNK-1 antibody with HCMV permissive cells prior to virus inoculation inhibi* plaque formation of HCMV,suggesting that the glycoconjugates containing HNK-1 carbohydrate epitope in* host cell have a special role in the process of HCMV infection.

在本研究中，我们发现先天性CMV感染患者的血清显示出针对硫酸化葡萄糖醛酸鞘糖脂（SGGL）的抗体滴度升高，SGGL含有单克隆抗体HN* 1反应性碳水化合物表位。SGPG在其nLc 4 * 的非还原端含有硫酸化葡萄糖醛酸，并且SGLPG具有类似的结构，但具有乳糖胺的额外单元。有趣的是，CMV感染患者血清中的抗体对SGLPG的反应性高于对SGPG的反应性，这种独特的特异性不同于其他HNK-1相关抗体。此外，抗硫苷脂和抗SG * 抗体均被硫苷脂缀合的辛基琼脂糖柱和肝素琼脂糖柱吸收，而CMV特异性IgG滴度不因抗硫酸化GL抗体的吸收而降低。结果表明，CMV感染可特异性地诱导抗硫酸盐抗体的产生，而这些抗体不同于CMV特异性抗体。纯化的HNK-1抗体可与HCMV阳性抗原和抗HNK-1抗体结合。用亲和层析法从病人血清中部分纯化的抗SGGL抗体也能与两种抗原结合，但其反应性低于抗HCMV抗体。这些结果表明，HNK-1表位的表达水平没有被HCMV感染改变，并且含有HNK-1表位的糖缀合物也存在于HCMV容许细胞中。此外，在病毒接种前用HCMV容许细胞预处理纯化的HNK-1抗体可抑制HCMV的空斑形成，表明宿主细胞中含有HNK-1糖表位的糖缀合物在HCMV感染过程中具有特殊作用。