STUDY OF MOLECULARBIOLOGY OF SEROTONIN RECEPTOR IN DEPRESSION

在中国仓鼠卵巢（CHO）细胞表达的5-<1C>HT_受体中研究了5-HT_受体介导的细胞内Ca^&lt;2+&gt;动员<1C>;并利用Fura-2/AM研究了5-HT_受体功能的调节<1C>。CHO细胞转染了5-HT_受体的cDNA克隆<1C>。加入5-HT以剂量依赖的方式动员细胞内Ca^&lt;2+&gt;。5-HT受体激动剂MCPP（1-（3-氯苯基）哌嗪二盐酸盐）<1C>和DOI（1-（4-碘-2，5-二甲氧基苯基）-2-氨基丙烷）（1-（4-iodo-2，5-dimethoxyphenyl）-2-aminopropane）（5-HT_<1C>和5-HT_2激动剂）暴露可导致细胞内Ca^&lt;2+&gt;水平升高。米安色林、美舒麦角、利坦色林和酮色林均比螺哌隆更有效地阻断5-HT介导的细胞内Ca^2+动员，米安色林和阿莫西林完全抑制5-HT介导的细胞内Ca^2+动员，阿米替林，结果提示，抗抑郁药对转染人5-HT_2受体的CHO细胞的拮抗作用<1C>是抗血清张力素能的作用。

Serotonin 5-HT_<1C> receptor-mediated intracellular Ca^<2+> mobilization was investigated in 5-HT_<1C> receptors expressed in chinese hamster ovary (CHO) cells ; and fura-2/AM was used to investigate the regulation of 5-HT_<1C> receptor function.CHO cells, transfected with a cDNA clone for the 5-HT_<1C> receptor.The addition of 5HT mobilized intracellular Ca^<2+> in a dose-dependent fashion.Exposure of 5HT,a 5-HT receptor agonist, MCPP (1-(3-chlorophenyl)piperazine dihydrochloride), a 5-HT _<1C> agonist and DOI (1-(4-iodo-2,5-dimethoxyphenyl)-2-aminopropane), a 5-HT_<1C> and 5-HT_2 agonist, resulted in increased intracellular Ca^<2+> levels.Mianserin, mesulergine, ritanserin and ketanserin each blocked 5-HT-mediated intracellular Ca^<2+> mobilization more effectively than spiperone.Mianserin and amoxapine inhibited 5-HT-mediated intracellular Ca^<2+> mobilization completely, amitryptyline, nortriptyline and imipramine reduced it about 50%.These results suggest that antagonism in CHO cells transfected with the human 5-HT_<1C> receptors is antiserortonergic effects of antidepressants.