Research on the DNA Recombinational Mutation and p53 Gene Mutation Correlating to the Development of Metastatic Potential in Human Cancer Cells

本研究的目的是探讨基于遗传不稳定性的DNA重组突变和p53基因突变在人癌细胞转移潜能形成中的意义。取4例晚期结直肠癌患者行结肠切除术后的外周血白细胞、肿瘤组织和正常结肠黏膜。从材料中提取DNA，用Southern杂交技术检测DNA重组突变。HPC-1是一种多态的小卫星探针，用于检测突变。对于P53基因突变，采用单链构象多态技术(SSCP)对7例患者进行分析。外显子6被用来检测突变的存在。与相应的WBC和正常粘膜相比，4例癌症标本没有表现出重组和/或DNA滑移导致的额外条带。这7例癌症标本也没有显示任何外显子6的突变。这些突变可能是由于样本数量不足(患者)以及可能不适合人类材料的分析方法所致。我们考虑了主题材料的积累和检测系统的修改。

The aim of this study was to investigate the significance of the DNA recombinational mutation based on the genetic instability and the p53 gene mutation in the development of metastatic potential in human cancer cells. Peripheral white blood cells (Wbc), tumor tissue and normal colonic mucosa were obtained from 4 patients undergoing colectomy for an advanced colorectal cancer. DNA were extracted from the materials and prepared to examine the DNA recombinational mutation with DNA fingerprinting by Southern hybridization technique. The hPc-1, a polymorphic minisatellite probe, was used to detect mutations. As to the p53 gene mutation, aterials were sampled from 7 patients and prepared for anlysis using th single strand confor-mation polymorphisms technique (SSCP) . The exon 6 was focused to examine the presence of mutation.The 4 cancer specimens exhibited no extra-bands resulting from recombination and/or DNA slippage as compared to the corresponding Wbc and normal mucosa. The 7 cancer specimens did not show any mutations either as to the exon 6. These nay resulted from inadequately small number of specimens (patients) and also, presumably, inappropriate analytic methodolgy for human materials. We consider the accumulation of subject materials and modification of assay systems.