Research on the DNA Recombinational Mutation and p53 Gene Mutation Correlating to the Development of Metastatic Potential in Human Cancer Cells

点击翻译按钮获取中文摘要

The aim of this study was to investigate the significance of the DNA recombinational mutation based on the genetic instability and the p53 gene mutation in the development of metastatic potential in human cancer cells. Peripheral white blood cells (Wbc), tumor tissue and normal colonic mucosa were obtained from 4 patients undergoing colectomy for an advanced colorectal cancer. DNA were extracted from the materials and prepared to examine the DNA recombinational mutation with DNA fingerprinting by Southern hybridization technique. The hPc-1, a polymorphic minisatellite probe, was used to detect mutations. As to the p53 gene mutation, aterials were sampled from 7 patients and prepared for anlysis using th single strand confor-mation polymorphisms technique (SSCP) . The exon 6 was focused to examine the presence of mutation.The 4 cancer specimens exhibited no extra-bands resulting from recombination and/or DNA slippage as compared to the corresponding Wbc and normal mucosa. The 7 cancer specimens did not show any mutations either as to the exon 6. These nay resulted from inadequately small number of specimens (patients) and also, presumably, inappropriate analytic methodolgy for human materials. We consider the accumulation of subject materials and modification of assay systems.