Post-transcriptional regulation associated with RNA modifications responsible for higher order biological processes

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This project aimed to study post-transcriptional regulation associated with RNA modifications responsible for higher order biological processes. We found that piwi-interacting RNAs (piRNA) expressed in mouse testis have 2'-O-methylation at their 3' termini, and characterized the methyltransferase responsible for this modification. Concerning mRNA modifications, we took notice on inosines that are abundantly introduced in RNAs from human brain. We have established a biochemical method, named "inosine chemical erasing (ICE)", to directly identify inosines on RNA strands. We have mapped more than 16,000 novel sites of inosines in human brain transcriptome. Furthermore, we have started a project of genome-wide identification of inosine sites by using the ICE-method combined with the deep sequencing (ICE-Seq).