Characterisation of membrane protein ubiquitination by MARCH ligases

MARCH 连接酶对膜蛋白泛素化的表征

• 批准号: DP160103134
• 负责人: Prof Jose Villadangos
• 金额: $ 44.31万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160103134
• 关键词: Characterisation; membrane; protein; ubiquitination; MARCH

The goal of the project is to understand how a family of enzymes called MARCHs regulate expression and localisation of immunoregulatory receptors within cells by post-translational addition of a small protein tag called Ubiquitin. The aims are to decipher the ubiquitination patterns produced by the MARCHs; identify the E2 ligases used by the MARCHs to produce distinct Ub codes; and apply a new proteomic pipeline to identify novel representative MARCH substrates in mice deficient in six different MARCHs. It is anticipated the project will reveal novel insights into a fundamental cell biological process of major significance for regulation of protein expression and trafficking in cells of the immune system.

该项目的目标是了解称为MARCHs的酶家族如何通过翻译后添加称为泛素的小蛋白质标签来调节细胞内免疫调节受体的表达和定位。目的是破译由MARCHs产生的泛素化模式；鉴定MARCHs使用E2连接酶来产生不同的Ub编码；并应用一种新的蛋白质组学管道，在6种不同的MARCH缺乏的小鼠中鉴定新的具有代表性的MARCH底物。预计该项目将揭示对免疫系统细胞中蛋白质表达和运输调节具有重要意义的基本细胞生物学过程的新见解。