Fundermental study on clinical application of BMP for endodontics

BMP是一种重要的生物活性蛋白，其生物活性是由递送系统促进的。在牙髓病学的临床应用中，BMP需要使用液体载体，角鲨烷易乳化，具有良好的扩散性。因此，角鲨烷在BMP递送系统中可能是有效的。将角鲨烷/BMP（牛源性部分纯化的）复合物植入ddY小鼠的肌周膜下。作为对照，仅使用BMP。种植体在1 ~ 4周时进行X线和组织学检查。角鲨烷/BMP复合材料标本的X线片显示广泛的异位成骨，经统计学分析，异位成骨量比对照组高40%左右。组织学检查显示2周时软骨膜骨化形成了不同数量的新骨。对3周和4周标本的骨观察表明，骨髓已经形成。这些数据表明角鲨烷对BMP的缓慢局部释放有效。

BMP is an important bioactive protein whose bioactivity is facilitated by the delivery system. In clinical application of endodontics, it is necessary to use fluid carrier for BMP.Squalane is easily emulsified and has the property of spreading well. Thus, squalane might be effective in a BMP delivery system. Squalane/BMP (bovine derived partially purified) composite were implanted under the perimuscular membrane of ddY mice. For control, BMP only were used. The implants were roentgenographically and histologically examined at 1 to 4 weeks. According to the roentgenographical analysis, squalane/BMP composite specimens showed widespread heterotopic bone formation, and the amount was about 40% greater than that in the controls by a statistically analysis. Histological examination revealed varying amounts of new bone formed by perichondral ossification by 2 weeks. Observation of the bone of 3- and 4-week specimens indicated that bone marrow had already formed. These data suggest that squalane is effective for the slow local release of BMP.