Fundermental study on clinical application of BMP for endodontics

BMP是一种重要的生物活性蛋白，其生物活性是由传递系统促进的。在牙髓学的临床应用中，有必要使用液体载体来实现BMP。角鲨烷易乳化，具有良好的涂抹性。因此，角鲨烷可能在BMP递送系统中有效。将角鲨烷/BMP（牛源性部分纯化）复合物植入小鼠肌周膜下。作为对照，只使用BMP。植入物于1至4周进行x线和组织学检查。经x线分析，角鲨烷/BMP复合标本显示广泛的异位骨形成，经统计分析，其量比对照组大40%左右。组织学检查显示，2周后软骨内骨化形成了不同数量的新骨。3周和4周标本的骨观察显示骨髓已形成。这些数据表明角鲨烷对BMP的局部缓慢释放是有效的。

BMP is an important bioactive protein whose bioactivity is facilitated by the delivery system. In clinical application of endodontics, it is necessary to use fluid carrier for BMP.Squalane is easily emulsified and has the property of spreading well. Thus, squalane might be effective in a BMP delivery system. Squalane/BMP (bovine derived partially purified) composite were implanted under the perimuscular membrane of ddY mice. For control, BMP only were used. The implants were roentgenographically and histologically examined at 1 to 4 weeks. According to the roentgenographical analysis, squalane/BMP composite specimens showed widespread heterotopic bone formation, and the amount was about 40% greater than that in the controls by a statistically analysis. Histological examination revealed varying amounts of new bone formed by perichondral ossification by 2 weeks. Observation of the bone of 3- and 4-week specimens indicated that bone marrow had already formed. These data suggest that squalane is effective for the slow local release of BMP.