Localization and Kinetics of Neural Transmitters in the Enteric Nervous System. Studies Using Laser Photoooagulations and Immunochemistry.

我们研究了神经再生。使用过氧化物酶-抗过氧化物酶复合物技术，通过免疫组织化学对豚鼠小肠进行氩离子激光照射，时间长达70天。形成直径约0.5mm的圆形激光病变，观察肠神经丛两种主要细胞类型的再生特征。即肠内神经元含有蛋氨酸-脑啡肽- arg ^6-Gly^7-Leu^8 （Enk^<-8>）等神经肽。Peptide-hisbydine-isoleucine(机器)。检测P物质（SP）和生长抑素（SM）及其含神经组织蛋白S-100b （S-100b蛋白）的肠胶质细胞。激光照射后立即。肠神经丛的组织学结构基本保持完整。尽管神经细胞和胶质细胞都凝固死亡。神经元和胶质细胞中神经肽和S-100b蛋白的免疫反应性也基本保持不变。在激光照射后3…多小时，神经残端对神经肽的免疫反应明显积累。3天后，逐渐将含有神经肽/S-100b蛋白的细胞碎片从病变中移除。由于神经元突起中的神经肽是通过快速的顺行轴突流从细胞体中转运出来的，因此病变口腔边缘神经肽的早期积累表明神经元向口-肛门方向投射突起，反之亦然。由此推断，在肌间神经丛中，Enk-8和SP神经元同时发出口头和肛门定向过程；而PHI和SM神经元主要在口-肛门方向发送过程。在深肌丛（DMP）中，Enk-8和SP神经元呈环状运行。在粘膜下神经丛中，PHI、SP和SM神经元的突起向各个方向均匀分布。激光照射后3 ~ 10天，病变周围被切除的神经细胞体的神经肽免疫反应性明显增强。这一现象被解释为肠神经元逆行变性的一种模式。在15至70天，再生的神经胸膜完全扩散到激光损伤的疤痕组织。病变神经节内未见神经细胞体。在再生的神经丛中，通过有丝分裂增殖的神经胶质细胞支持和引导从病变周围神经节存活的神经元细胞体延伸出来的神经纤维。这表明，在非实验条件下，在肠神经丛自主神经丛的持续重塑中，激光病变中的特征性神经元/胶质细胞相互作用也可能发生。少

We investigated nerve regeneration. following lesion forming Argon ion laser irradiation in the guinea-pig small intestine by immunohistochemistry using peroxidase-antiperoxidase complex techniques over a time course of up to 70 days.Round laser-lesions about 0.5mm in diameter were produced and the regenerative features of the two major cell types of the enteric nerve plexuses. i.e. enteric neurons containing neuropeptide by such as methionine-enkephalin-Arg^6-Gly^7-Leu^8 (Enk^<-8>).Peptide-hisbydine-isoleucine (PIII). substance P (SP) and somatostatin (SM) together with tby enteric glial cells containing nerve tissue protein S 100b (S-100b protein by were examined.Immediately after the laser irradiation. the histological structure of the enteric nerve plexuses remained almost intact. though both neuronal and glial cells were coagulated and dead. The immunoreactivities for neuropeptides and S-100b proteins in the neuronal and glial elements respectively were also mostly preserved. By 3 … More hrs after the laser irradiatio, a conspicuous accumulation of immunoreactivities to neuropeptides occurred in the severed nerve stumps. The cellular debris, containing neuropeptides/S-100b protein, were gradually removed from the lesion by 3 days. Since neuropeptides in the neuronal processes are transported from the cell body by the fast anterograde axonal flow, an early accumulation of neuropeptides on the oral edge of the lesion indicates that the neurons project processes in the oro-anal direction, and vice versa.It was deduced that, in the myenteric plexus, both Enk-8 and SP neurons issued both orally and anally directed processes; whereas, PHI and SM neurons sent processes mainly in the oro-anal direct-ion. In the deep muscular plexus (DMP), Enk-8 and SP neurons ran circularly. In the submucous plexus, projections of PHI, SP and SM neurons were directed evenly in all directions.Neuropeptide immunoreactivities of the axotomized nerve cell bodies in the area surrounding the lesion became strikingly stronger 3 to 10 days after laser irradiation. This phenomenon was interpreted as a mode of retrograde degeneration of the enteric neurons. At 15 to 70 days, regenerated nerve plekuses completely spread over the scar tissue of the laser lesion. No nerve cell body, however, existed in the lesioned ganglia. In the regenerated nerve plexus, the glial cells, which proliferated by mitosis, supported and guided the nerve fibers which extended from the survived neuronal cell bodies in the ganglia around the lesion. This suggests that the characteristic neuron/glial cell interactions in the laser lesion might also occur daring the continuous remodeling of the autonomic groundplexus under non-experimental conditions in the enteric nerve plexuses. Less