Localization and Kinetics of Neural Transmitters in the Enteric Nervous System. Studies Using Laser Photoooagulations and Immunochemistry.
肠神经系统中神经递质的定位和动力学。
基本信息
- 批准号:62480093
- 负责人:
- 金额:$ 4.29万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1987
- 资助国家:日本
- 起止时间:1987 至 1989
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We investigated nerve regeneration. following lesion forming Argon ion laser irradiation in the guinea-pig small intestine by immunohistochemistry using peroxidase-antiperoxidase complex techniques over a time course of up to 70 days.Round laser-lesions about 0.5mm in diameter were produced and the regenerative features of the two major cell types of the enteric nerve plexuses. i.e. enteric neurons containing neuropeptide by such as methionine-enkephalin-Arg^6-Gly^7-Leu^8 (Enk^<-8>).Peptide-hisbydine-isoleucine (PIII). substance P (SP) and somatostatin (SM) together with tby enteric glial cells containing nerve tissue protein S 100b (S-100b protein by were examined.Immediately after the laser irradiation. the histological structure of the enteric nerve plexuses remained almost intact. though both neuronal and glial cells were coagulated and dead. The immunoreactivities for neuropeptides and S-100b proteins in the neuronal and glial elements respectively were also mostly preserved. By 3 … More hrs after the laser irradiatio, a conspicuous accumulation of immunoreactivities to neuropeptides occurred in the severed nerve stumps. The cellular debris, containing neuropeptides/S-100b protein, were gradually removed from the lesion by 3 days. Since neuropeptides in the neuronal processes are transported from the cell body by the fast anterograde axonal flow, an early accumulation of neuropeptides on the oral edge of the lesion indicates that the neurons project processes in the oro-anal direction, and vice versa.It was deduced that, in the myenteric plexus, both Enk-8 and SP neurons issued both orally and anally directed processes; whereas, PHI and SM neurons sent processes mainly in the oro-anal direct-ion. In the deep muscular plexus (DMP), Enk-8 and SP neurons ran circularly. In the submucous plexus, projections of PHI, SP and SM neurons were directed evenly in all directions.Neuropeptide immunoreactivities of the axotomized nerve cell bodies in the area surrounding the lesion became strikingly stronger 3 to 10 days after laser irradiation. This phenomenon was interpreted as a mode of retrograde degeneration of the enteric neurons. At 15 to 70 days, regenerated nerve plekuses completely spread over the scar tissue of the laser lesion. No nerve cell body, however, existed in the lesioned ganglia. In the regenerated nerve plexus, the glial cells, which proliferated by mitosis, supported and guided the nerve fibers which extended from the survived neuronal cell bodies in the ganglia around the lesion. This suggests that the characteristic neuron/glial cell interactions in the laser lesion might also occur daring the continuous remodeling of the autonomic groundplexus under non-experimental conditions in the enteric nerve plexuses. Less
我们研究了神经再生。用氩离子激光照射豚鼠小肠70天后,用过氧化物酶-抗过氧化物酶复合物免疫组织化学方法,观察到直径约0.5mm的圆形激光损伤和肠神经丛两种主要细胞类型的再生特征。即肠神经元含有神经肽,如甲硫氨酸-脑啡肽-Arg^6-Gly^7-Leu^8(Enk^<-8>)、肽-组氨酸-异亮氨酸(PIII)。激光照射后立即检测肠神经胶质细胞P物质(SP)、生长抑素(SM)和神经组织蛋白S-100 b(S-100 b蛋白)的含量。肠神经丛的组织学结构几乎保持完整。尽管神经元和神经胶质细胞都凝固并死亡。神经肽和S-100 b蛋白在神经元和胶质细胞成分中的免疫反应也大部分保留。了3 ...更多信息 激光照射后3 h,神经残端内神经肽免疫反应性明显增多。细胞碎片,含有神经肽/S-100 b蛋白,逐渐从病变3天。由于神经元突起中的神经肽是通过快速的顺行轴突流从胞体中转运出来的,因此早期在病变口侧边缘的神经肽的积累表明神经元突起是向口-肛方向投射的,反之亦然,推测在肌间神经丛中,Enk-8和SP神经元都向口-肛方向投射突起;而PHI和SM神经元主要在口-肛方向发出信息。在深肌丛(Deep muscular plexus,DRG),Enk-8和SP神经元呈环状走行。在粘膜下神经丛中,PHI、SP和SM神经元的投射均匀地指向各个方向,激光照射后3 ~ 10天,病变周围区域轴突切断的神经细胞体的神经肽免疫反应明显增强。这种现象被解释为肠神经元的逆行变性模式。在15至70天,再生的神经丛完全遍布激光损伤的瘢痕组织。然而,在受损的神经节中不存在神经细胞体。在再生的神经丛中,通过有丝分裂增殖的胶质细胞支持和引导从损伤周围神经节中存活的神经元胞体延伸的神经纤维。这表明,在非实验条件下,在肠神经丛中,在自主神经基础丛的持续重塑过程中,激光损伤中的特征性神经元/神经胶质细胞相互作用也可能发生。少
项目成果
期刊论文数量(54)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
小林繁: "末梢神経、特に腸管神経叢の組織科学" 組織細胞化学1988. 155-167 (1988)
Shigeru Kobayashi:“周围神经的组织科学,特别是肠丛”组织细胞化学 1988. 155-167 (1988)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
小林繁: "腸管神経叢の形態" 日本小児外科学会雑誌. 24. 1411-1425 (1988)
Shigeru Kobayashi:“肠丛的形态学”日本小儿外科学会杂志 24. 1411-1425 (1988)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
S.Kobayashi;J.B.Furness;T.K.Smith;S.Pompolo: Arch.Histol.Cytol.52. (1989)
S.Kobayashi;J.B.Furness;T.K.Smith;S.Pompolo:Arch.Histol.Cytol.52。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Shigeru Kobayashi: "Ultrastructure of the nerve plexus in Oddi's sphincter of the guinea pig." Gastrointestinal Function, 5, 44-52,1987.
Shigeru Kobayashi:“豚鼠 Oddi 括约肌神经丛的超微结构。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tsuneo Fujita: "The Paraneuron" Springer-Verlag, 367p (1989)
藤田恒夫:《副神经元》Springer-Verlag,367p (1989)
- DOI:
- 发表时间:
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- 影响因子:0
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KOBAYASHI Shigeru其他文献
Controlling the Ionic and Electronic Transport at the All-solid-state Battery Interfaces
控制全固态电池接口的离子和电子传输
- DOI:
10.1380/vss.64.542 - 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
KOBAYASHI Shigeru;NISHIO Kazunori;SHIRASAWA Tetsuroh;HITOSUGI Taro - 通讯作者:
HITOSUGI Taro
KOBAYASHI Shigeru的其他文献
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{{ truncateString('KOBAYASHI Shigeru', 18)}}的其他基金
Half Autonomous Control of Multi Robots against Disaster and Teleoperation via Internet
多机器人抗灾半自主控制及网络远程操作
- 批准号:
25282108 - 财政年份:2013
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Effect of Alendronate on the metabolism of cartilaginous and disc cells in vitro
阿仑膦酸钠对体外软骨细胞和椎间盘细胞代谢的影响
- 批准号:
21591896 - 财政年份:2009
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$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Compilation of maps, aerial photographs and meteorological records produced by former Japanese military for the monitoring of environmental change in the Asian and Pacific Areas
前日本军方为监测亚太地区环境变化而制作的地图、航空照片和气象记录汇编
- 批准号:
19200059 - 财政年份:2007
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$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
A problem of learning and cultural activities for the person with a mental disorder in the community life support
社区生活支持中精神障碍患者的学习和文化活动问题
- 批准号:
19530723 - 财政年份:2007
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The positive research regarding the lifelong learning of the person with disabilities-from point of view of the right to learn-
关于残疾人终身学习的实证研究——从学习权的角度看——
- 批准号:
16530529 - 财政年份:2004
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Study of Genetic Adaptation to Malaria in Nepal-In Comparison with Cultural Adaptation
尼泊尔疟疾遗传适应研究——与文化适应的比较
- 批准号:
15406009 - 财政年份:2003
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Fundamental Study on the Maps of Asia-Pacific Areas Prepared by the Japanese Army
日本陆军绘制的亚太地区地图的基础研究
- 批准号:
14208007 - 财政年份:2002
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
The Study of Cultural and Biological Adaptations to Malaria in Nepal
尼泊尔对疟疾的文化和生物适应研究
- 批准号:
12575016 - 财政年份:2000
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
REGIONAL VARIATIONS IN THE RYUKYU ISLANDERS ON TOOTH CROWN TRAITS
琉球岛民牙冠特征的地区差异
- 批准号:
10671705 - 财政年份:1998
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research of automatic structure which appear in algebras
代数中出现的自动结构的研究
- 批准号:
10640030 - 财政年份:1998
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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