Parallel and convergent neuronal connections and neuroactive substances

平行和会聚的神经元连接和神经活性物质

基本信息

批准号：
62480098
负责人：
MIZUNO Noboru
金额：
$ 1.6万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

1. Distribution of putative glutamatergic neurons in the cerbral cortex, thalamus, lower brainstem and cerebellum of the rat was examined immunohistochemically by using a monoclonal antibody against phosphate-activated glutaminase (PAG), which has been proposed to be a major synthetic enzyme of transmitter glutamate. Intensely-stained ueuronal cell bodies were seen in the pyramidal cells in layers V and VI in the neocortex and mesocortex, and pyramidal cells in Ammon's horn, neurons in the main precerebellar nuclei sending mossy-fibers to the cerebellum. PAG was also shown in many monoaminergic neurons by a double-staining method using the anti-glutaminase antibody combined with an antiserum against TH, DBH,PNMT or serotonin.2. Convergence of serotonin (5HT)-, enkephalin (ENK)- and substance P (SP)-like immuno reactive (LI) fibers on single pudendal motoneurons of Onuf's nucleus was shown by retrogradely labeling neurons in Onuf's nucleus with HRP, and then by immunohistochemically lab … More eling 5HT-, ENK- and SP-fibers in the same sections using distinct chromogen. Similar studies were also done on the medullary dorsal horn, mesencephalic trigeminal nucleus, and lumbar spinal cord.3. Cholecystokinin (CCK)- and/or vasoactive intestinal polypeptide (VIP)-LI was shown in many ceurons in the thalmic VL neurons which were retrogradely labeled with WGA-HRP injected into the frontal cortex of the rat. VIP- or CCK-LI was observed in 70-80% or 50-60% of thalamocortical VL neurons, respectively. Almost all of thalamocortical VL neurons with CCK-LI exhibited VIP-LI.4. Many fibers with calcitonin gene-related peptide (CGRP)-LI in the cerebellum of the cat were demonstrated to arise from the pontine nuclei: when a lesion was placed in the pontine prachium, mossy fiber endings with CGRP-LI were reduced in number in the cerebellar hemis phere of the posterior lobe ipsilateral to the lesion. Fine varicose fibers with CGRP-LI within the dentate nucleus were also decreased in number ipsilateral to the lesion.5. A combined method of the tracing of WGA-HRP and the immunohistochemistry of substance P revealed that many SP-LU neurons in the supramammillary nucleus of cat hypothalamus sent their axons to the hippocampal formation: SP-LI axons in the hippocampal formation abd ento rhinal cortex were markedly resuced in number ipilaterally after placing an electrothermic lesion in the supramammillary region of the hypothalamus. Less

1。通过对磷酸激活的谷氨酸（PAG）的单克隆抗体进行免疫组织化学检查，对大鼠的Cerbral皮层，丘脑，脑干和小脑的分布进行了分布，并已提议是主要的合成enz eNzeme transterters grougamemate grougamemate grougamemate。在新皮层和中皮层中的层VI中，在锥体细胞中观察到染色的ueuronal细胞体，以及Ammon Horn的锥体细胞，主要前脑核核核中的神经元中的神经元，将苔藓纤维发送到小脑。使用抗谷氨酰胺酶抗体与抗Th，dbh，pNMT或5-羟色胺相结合的抗谷氨酰胺酶抗体在许多单胺能神经元中也显示了PAG。2。 5-ht（5HT） - ，Enkephalin（ENK）和物质P（SP）类似于Onuf核的单个底部运动神经元对Onuf的神经元与HRP的神经元的逆转来显示，然后通过免疫组合物在Onuf的神经元中逆转来显示，并通过免疫组织逆转来表明ONUF核的单个Pudendal运动神经元上的纤维和纤维的收敛。在相同的部分中，使用不同的发色原。也对髓质背角，脑脑三叉神经核和腰脊髓进行了类似的研究。3。胆囊基蛋白（CCK） - 和/或血管活性肠多肽（VIP）-Li在丘脑VL神经元中的许多Ceuron中显示出，这些神经元的WGA-HRP被逆行标记，并注射到大鼠额叶的额叶皮质中。在70-80％或50-60％的丘脑皮质VL神经元中观察到了VIP-或CCK-LI。几乎所有带有CCK-LI暴露VIP-LI.4的丘脑皮质VL神经元。 Many fibers with calcitonin gene-related peptide (CGRP)-LI in the cerebellum of the cat were demonstrated to arise from the Pontine Nuclei: when a lesion was placed in the pontine prachium, mossy fiber endings with CGRP-LI were reduced in number in the cerebellar hemis phere of the posterior lobe ipsilateral to the lesion.齿状核核内具有CGRP-LI的细静脉曲纤维在病变的同侧数也得到了改善。5。 PAT下丘脑上丘脑上的许多SP-LU神经元在CAT下丘脑上核核us中的许多SP-LU神经元在猫下丘脑的核核中的许多SP-LU神经元将其轴突带到海马形成：海马形成：海马形成Abd obd obd ofto abd obd of abd obd of abd obd ob abd obd ob abd obd ob abd obd ob abd obd ob abd obd ob abd obd ob abd obd oft ip ipire abd obd obs ip ipire，abd obd obd obs efter在数字中，下丘脑的上区域。较少的