Parallel and convergent neuronal connections and neuroactive substances

平行和会聚的神经元连接和神经活性物质

• 批准号: 62480098
• 负责人: MIZUNO Noboru
• 金额: $ 1.6万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480098/
• 关键词: Glutaminase; Enkephalin (ENK); Substance P (SP); Glutamate; Catecholamine; Cholecystokinin (CCK); Serotonin; Vasoactive intestinal polypeptide (VIP); グルタミン酸作動性ニュ-ロン; 小脳; 脳幹; セロトニン; カテコ-ルアミン; 免疫組織化学; アセチルコリンエステラーゼ; チロシン水酸化酵素; 下立; 聴覚系; 自律神

1. Distribution of putative glutamatergic neurons in the cerbral cortex, thalamus, lower brainstem and cerebellum of the rat was examined immunohistochemically by using a monoclonal antibody against phosphate-activated glutaminase (PAG), which has been proposed to be a major synthetic enzyme of transmitter glutamate. Intensely-stained ueuronal cell bodies were seen in the pyramidal cells in layers V and VI in the neocortex and mesocortex, and pyramidal cells in Ammon's horn, neurons in the main precerebellar nuclei sending mossy-fibers to the cerebellum. PAG was also shown in many monoaminergic neurons by a double-staining method using the anti-glutaminase antibody combined with an antiserum against TH, DBH,PNMT or serotonin.2. Convergence of serotonin (5HT)-, enkephalin (ENK)- and substance P (SP)-like immuno reactive (LI) fibers on single pudendal motoneurons of Onuf's nucleus was shown by retrogradely labeling neurons in Onuf's nucleus with HRP, and then by immunohistochemically lab … More eling 5HT-, ENK- and SP-fibers in the same sections using distinct chromogen. Similar studies were also done on the medullary dorsal horn, mesencephalic trigeminal nucleus, and lumbar spinal cord.3. Cholecystokinin (CCK)- and/or vasoactive intestinal polypeptide (VIP)-LI was shown in many ceurons in the thalmic VL neurons which were retrogradely labeled with WGA-HRP injected into the frontal cortex of the rat. VIP- or CCK-LI was observed in 70-80% or 50-60% of thalamocortical VL neurons, respectively. Almost all of thalamocortical VL neurons with CCK-LI exhibited VIP-LI.4. Many fibers with calcitonin gene-related peptide (CGRP)-LI in the cerebellum of the cat were demonstrated to arise from the pontine nuclei: when a lesion was placed in the pontine prachium, mossy fiber endings with CGRP-LI were reduced in number in the cerebellar hemis phere of the posterior lobe ipsilateral to the lesion. Fine varicose fibers with CGRP-LI within the dentate nucleus were also decreased in number ipsilateral to the lesion.5. A combined method of the tracing of WGA-HRP and the immunohistochemistry of substance P revealed that many SP-LU neurons in the supramammillary nucleus of cat hypothalamus sent their axons to the hippocampal formation: SP-LI axons in the hippocampal formation abd ento rhinal cortex were markedly resuced in number ipilaterally after placing an electrothermic lesion in the supramammillary region of the hypothalamus. Less

1.用磷酸活化谷氨酸胺酶（PAG）单克隆抗体，对大鼠大脑皮质、丘脑、脑干下部和小脑内谷氨酸能神经元的分布进行了免疫组织化学研究。在新皮质和中皮质的V层和VI层锥体细胞、阿蒙角的锥体细胞、小脑前主要核团中向小脑发送苔藓纤维的神经元中可见染色强烈的神经元胞体。用抗β-内酰胺酶抗体和抗TH、DBH、PNMT或β-内酰胺酶的抗血清进行双染，结果表明PAG在许多单胺能神经元中也有表达.用HRP逆行标记大鼠阴部Onuf核内单个运动神经元，并用免疫组化方法观察了5-羟色胺（5-HT）、脑啡肽（ENK）和P物质（SP）样免疫反应（LI）纤维在Onuf核内的会聚。 ...更多信息 用不同的色原在同一切片中分离5 HT-、ENK-和SP-纤维。对延髓背角、三叉神经中脑核和腰髓也进行了类似的研究。用WGA-HRP逆行标记大鼠额叶皮质丘脑VL神经元，在许多神经元内可见到胆囊收缩素（CCK）和/或血管活性肠肽（VIP）阳性反应。VIP和CCK-LI分别见于70-80%和50-60%的丘脑皮质VL神经元。几乎所有CCK阳性的丘脑皮质VL神经元均呈VIP阳性。许多纤维与降钙素基因相关肽（CGRP）-LI在猫的小脑被证明是从脑桥核：当一个病变被放置在脑桥的pachium，苔藓纤维末梢与CGRP-LI减少在同侧的后叶小脑半球的病变。同侧齿状核内CGRP-LI阳性的曲张细纤维也减少.应用WGA-HRP示踪和SP免疫组化相结合的方法，发现猫下丘脑乳头体上核内有大量SP-LU神经元向海马结构发出轴突：电毁损下丘脑乳头体上核后，同侧海马结构和鼻内皮质内SP-LU神经元数目明显减少。少

项目成果

Yasui,Yukihiko: "The cingulate gyrus of the cat receives projection fibers from the thalamic region ventral to the ventral border of the ventrobasal complex in the cat" Journal of Comparative Neurology. 274. 91-100 (1988)

安井幸彦：“猫的扣带回接收从丘脑区腹侧到猫腹基底复合体腹侧边缘的投射纤维”《比较神经学杂志》。

Sugimoto, Tetsuo: "Calcitonin gene-related peptide-like immunoreactivity in neuronal elements of the cat cerebellum" Brain Res., 439: 147-154, 1988.

Sugimoto, Tetsuo：“猫小脑神经元中降钙素基因相关的肽样免疫反应性”Brain Res.，439：147-154，1988。

Tashiro, Takashi: "Distribution of axons exhibiting both enkephalin- and serotonin-like immunoreactivities in the lumbar cord segments: an immunohistochemical study in the cat" Brain Res., 440: 357-362, 1988.

Tashiro, Takashi：“腰脊髓段中表现出脑啡肽和血清素样免疫反应性的轴突分布：猫的免疫组织化学研究”Brain Res.，440：357-362，1988。

Yasui, Yukihiko: "The cingulate gyrus of the cat receives projection fibers from the thalamic region ventral to the ventral to the ventral border of the ventrobasal complex in the cat" J. Comp. Neurol., 274: 91-100, 1988.

Yasui，Yukihiko：“猫的扣带回接收从丘脑区域腹侧到腹侧到猫腹基底复合体腹侧边界的投射纤维”J. Comp。

Kaneko, Takeshi: "Correlation between immunochemical characteristics and immunohistochemical applicability of nine lines of monoclonal antibodies against rat brain glutaminase" J. Histochem. Cytochem., 36: 997-1004, 1988.

Kaneko，Takeshi：“九种抗大鼠脑谷氨酰胺酶单克隆抗体的免疫化学特征与免疫组织化学适用性之间的相关性”J. Histochem。