Neuron circuit analysis on the basis of neuroactive substances and receptors

1.制备P物质受体(SPR)抗体，用免疫组织化学方法检测SPR在大鼠中枢神经系统(CNS)的分布。SPR的大脑皮层神经元绝大多数为GABA能非锥体神经元。用原位杂交法检测代谢性谷氨酸受体mGluR2-mGluR7的mRNAs在大鼠中枢神经系统的分布。抗mGluR2和mGluR4-mGluR8抗体的产生。免疫组织化学光镜和电子显微镜观察m-GluR在小脑皮质(mGluR2和mGluR4)、脊髓I层(MGluR7)和嗅结节(MGluR8)的突触前定位。在光镜和电子显微镜下观察到MOR的突触前定位在副视束内侧终核、孤束核和脊髓I层。在脊髓的I层，含有P物质的轴突终末含有MOR4。对大脑皮层、纹状体和小脑皮质的局部环路、伤害性感觉系统、内源性痛觉控制系统和咀嚼系统进行了分析。

1.An antibody against substance P receptor (SPR) was raised, and distribution of SPR in the central nervous system (CNS) of the rat was examined immunohistochemically. The vast majority of cerebral cortical neurons with SPR were GABAergic non-pyramidal neurons. Striatal neurons with SPR were segregated into somatostatinergic and cholinergic apiny (intrinsic) neurons.2.Distributions of mRNAs for metabotropic glutamate receptors, mGluR2-mGluR7 in the rat CNS were examined by in situ hybridization. Antibodies against mGluR2, and mGluR4 - mGluR8 were raised. Presynaotic localization of m GluRs were immunohistochemically observed light and electron microscopically in the cerebellar cortex (mGluR2 and mGluR4) , layr I of the spinal cord (mGluR7) and olfactory tubercle (mGluR8).3.Two kinds of antibodies against mu-opioid receptor (MOR) were raised, and distribution of MOR in the rat CNS was examined immunohistochemically. Presynaptic localization of MOR was observed light and electron microscopically in the medial terminal nucleus of the accessory optic tract, nucleus of the solitary tract, and layr I of the spinal cord. In layr I of the spinal cord, some axon terminalswith substance P contained MOR.4.Analysis was performed on the local circuitry in the cerebral cortex, striatum and cerebellar cortex, the nociceptive system, the endogeous pain-control system, and masticatory system.