Neuron circuit analysis on the basis of neuroactive substances and receptors

基于神经活性物质和受体的神经元回路分析

• 批准号: 05454658
• 负责人: MIZUNO Noboru
• 金额: $ 4.22万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454658/
• 关键词: cerebral cortex; striatum; cerebellum; pain; endogenous paincontrol system; opioid receptor; metabotropic glutamate receptor; substance P receptor; グルタミン酸合成酵素; K物質受容体; 排尿中枢; 顎運動; 迷走神経核群; NMDA受容体; P物質; セロトニン; ドパミン; GABA; 網膜

1.An antibody against substance P receptor (SPR) was raised, and distribution of SPR in the central nervous system (CNS) of the rat was examined immunohistochemically. The vast majority of cerebral cortical neurons with SPR were GABAergic non-pyramidal neurons. Striatal neurons with SPR were segregated into somatostatinergic and cholinergic apiny (intrinsic) neurons.2.Distributions of mRNAs for metabotropic glutamate receptors, mGluR2-mGluR7 in the rat CNS were examined by in situ hybridization. Antibodies against mGluR2, and mGluR4 - mGluR8 were raised. Presynaotic localization of m GluRs were immunohistochemically observed light and electron microscopically in the cerebellar cortex (mGluR2 and mGluR4) , layr I of the spinal cord (mGluR7) and olfactory tubercle (mGluR8).3.Two kinds of antibodies against mu-opioid receptor (MOR) were raised, and distribution of MOR in the rat CNS was examined immunohistochemically. Presynaptic localization of MOR was observed light and electron microscopically in the medial terminal nucleus of the accessory optic tract, nucleus of the solitary tract, and layr I of the spinal cord. In layr I of the spinal cord, some axon terminalswith substance P contained MOR.4.Analysis was performed on the local circuitry in the cerebral cortex, striatum and cerebellar cortex, the nociceptive system, the endogeous pain-control system, and masticatory system.

1.制备了抗P物质受体（SPR）的抗体，用免疫组织化学方法观察了SPR在大鼠中枢神经系统（CNS）的分布。SPR的大脑皮质神经元绝大多数为GABA能非锥体神经元。2.原位杂交检测代谢型谷氨酸受体mGluR 2-mGluR 7在大鼠中枢神经系统的分布。产生了针对mGluR 2和mGluR 4-mGluR 8的抗体。免疫组织化学方法观察mGluRs在小脑皮质（mGluR 2和mGluR 4）、脊髓Ⅰ层（mGluR 7）和嗅结节（mGluR 8）的突触前定位。光镜和电镜下观察到莫尔的突触前定位于副视束内侧终核、孤束核和脊髓I层。在脊髓I层，一些含有P物质的轴突终末含有MOR。4.对大脑皮层、纹状体和小脑皮层的局部回路、伤害性感受系统、内分泌痛控制系统和咀嚼系统进行了分析。

项目成果

大石 仁: "Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex" Neuron. 13. 55-66 (1994)

Hitoshi Oishi：“大鼠小脑皮质中代谢型谷氨酸受体 mGluR2 和 mGluR3 的免疫组织化学定位”Neuron。13. 55-66 (1994)

李雲慶: "Premptor neurons for trigeminal motor nucleus neurons innervating the jaw-closing and jaw-opening muscles: Differential distribution in the lowor brainstem of the rat" J. Comp. Neurol.356. 563-579 (1995)

李云清：“支配下颌和张开肌肉的三叉神经运动核神经元：大鼠下脑干的差异分布”J. Comp. 563-579（1995）。

重本 隆一: "Immunohistochemical localization of a metal-otropic glutamate receptor,mGluR5,in the rat brain" Neurosci.Lett.163. 53-57 (1993)

Ryuichi Shigemoto：“大鼠大脑中金属型谷氨酸受体 mGluR5 的免疫组织化学定位”Neurosci.Lett.163 (1993)。

赤沢 千尋: "Expression of mRNAs of L-AP4 sensitive metal-otropic glutamute receptors(mGluR4,mGluR6,mGluR7)in the rat retina" Neurosci Lett.(印刷中). (1994)

Chihiro Akazawa：“大鼠视网膜中 L-AP4 敏感金属谷氨酸受体（mGluR4、mGluR6、mGluR7）的 mRNA 表达”Neurosci Lett（出版中）。

金子 武嗣: "Glutamate-synthesizing enzymas in GABAergic neurons of the neocortex: a double immunofluorescence study in the rat" Neuroscience. 61. 839-849 (1994)

Takeshi Kaneko：“新皮质 GABA 能神经元中的谷氨酸合成酶：大鼠的双重免疫荧光研究”《神经科学》61. 839-849 (1994)。