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Neuronal connection of the nociceptive and pain-modulating systems, with special reference to peptides and their receptors involved in the systems.

伤害感受和疼痛调节系统的神经元连接，特别涉及系统中涉及的肽及其受体。

基本信息

批准号：
10680701
负责人：
MIZUNO Noboru
金额：
$ 2.43万
依托单位：
TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH (1999)Tokyo Metropolitan Institute for Neuroscience (1998)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. NK1-immunoreactive neurons sending their axons to the periaqueductal gray were distributed mainly in lamina I of the medullary and spinal dorsal horns, spinal lateral nucleus, and additionally in laminae V and X of the spinal cord in the rat.2. Distribution of NK1-immunoreactivity was examined in the newborn and adult human spinal cords.3. Mu-opioid receptor-immunoreactivity was found in substance P- or CGRP-immunoreactive axon terminals in laminae I and II of the medullary and spinal dorsal horn of the rat.4. Preprodynorphin-immunoreactivity was found in some trigeminothalamic neurons in the medullary dorsal horn of the rat.5. In intracellular recording and labeling experiments in slice preparations of the rat medulla oblongata, local circuit neurons in lamina II of the medullary dorsal horn were divided into two types on the basis of their electrical membrane properties and axonal arborization patterns. A population of the local circuit neurons of one type showed calbindin D28K-immunoreactivity.6. Immunoreactivities for calcium-binding proteins (parvalbumin, calretinin, calbindin D28K) were observed in medullary dorsal horn neurons which send their axons to the parabrachial, thalamic, or hypothalamic regions in the rat. Some of these neurons were confirmed to express c-Fos-immunoreactivity when 10% formalin was injected subcutaneously into the perioral regions.

1. 向导水管周围灰质发送轴突的nk1免疫反应神经元主要分布在髓脊角和脊髓背角I层、脊髓外侧核，另外还分布在脊髓V层和X层。在新生儿和成人脊髓中检测了nk1免疫反应性的分布。3 .大鼠脊髓和脊髓背角I和II层的P-物质或cgrp免疫反应性轴突终末可见mu -阿片受体免疫反应性。在大鼠髓质背角部分三叉丘脑神经元中发现前脑啡肽免疫反应性。在大鼠延髓切片制备的细胞内记录和标记实验中，根据其电膜特性和轴突树突模式将延髓背角II层局部回路神经元分为两类。一种类型的局部回路神经元群体显示calbindin d28k免疫反应性。钙结合蛋白（小白蛋白、calretinin、calbindin D28K）在向大鼠臂旁、丘脑或下丘脑区域发送轴突的髓背角神经元中观察到免疫反应性。当10%福尔马林皮下注射到口腔周围区域时，证实部分神经元表达c- fos免疫反应性。