Neuronal connection of the nociceptive and pain-modulating systems, with special reference to peptides and their receptors involved in the systems.

伤害感受和疼痛调节系统的神经元连接，特别涉及系统中涉及的肽及其受体。

基本信息

批准号：
10680701
负责人：
MIZUNO Noboru
金额：
$ 2.43万
依托单位：
TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH (1999)Tokyo Metropolitan Institute for Neuroscience (1998)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. NK1-immunoreactive neurons sending their axons to the periaqueductal gray were distributed mainly in lamina I of the medullary and spinal dorsal horns, spinal lateral nucleus, and additionally in laminae V and X of the spinal cord in the rat.2. Distribution of NK1-immunoreactivity was examined in the newborn and adult human spinal cords.3. Mu-opioid receptor-immunoreactivity was found in substance P- or CGRP-immunoreactive axon terminals in laminae I and II of the medullary and spinal dorsal horn of the rat.4. Preprodynorphin-immunoreactivity was found in some trigeminothalamic neurons in the medullary dorsal horn of the rat.5. In intracellular recording and labeling experiments in slice preparations of the rat medulla oblongata, local circuit neurons in lamina II of the medullary dorsal horn were divided into two types on the basis of their electrical membrane properties and axonal arborization patterns. A population of the local circuit neurons of one type showed calbindin D28K-immunoreactivity.6. Immunoreactivities for calcium-binding proteins (parvalbumin, calretinin, calbindin D28K) were observed in medullary dorsal horn neurons which send their axons to the parabrachial, thalamic, or hypothalamic regions in the rat. Some of these neurons were confirmed to express c-Fos-immunoreactivity when 10% formalin was injected subcutaneously into the perioral regions.

1。将其轴突发送到周围灰色的NK1-免疫反应性神经元主要分布在髓质和脊柱背角的椎板I中，脊髓外侧核，并在大鼠脊髓的椎板V和X中另外分布。在新生儿和成年人类脊髓中检查了NK1免疫反应性的分布3。在大鼠的髓质I和II中发现了Mu-Apioid受体免疫反应性。在大鼠的髓质背角中的一些三角形神经元中发现了前肾上非 - 免疫反应性。5。在大鼠髓质长圆形的切片制剂中的细胞内记录和标记实验中，根据其电膜性质和轴突植物植物模式，将髓质背喇叭层中的局部回路神经元分为两种类型。一种类型的局部回路神经元的人群显示Calbindin D28K-免疫反应性6。在髓质背侧角神经元中观察到钙结合蛋白的免疫反应性（白蛋白，钙朋友蛋白，钙蛋白素，钙蛋白原D28K），它们将轴突发送到大鼠大鼠中的瘫痪，丘脑或下丘脑区域。当将10％福尔马林皮下注射到周围区域时，这些神经元中的一些被证实表达C-FOS免疫反应性。