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Morphological studies on neuronal and molecular features of sensory input-controls

感觉输入控制的神经元和分子特征的形态学研究

基本信息

批准号：
12680743
负责人：
MIZUNO Noboru
金额：
$ 2.3万
依托单位：
TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680743/
关键词：
spinal nucleus of trageminal mesencephalic uncleus of trrigeminal nerve raphe nuclei NK1 receptor GABA receptor calcium-binding protein protein kinase C pain 三叉神経感覚核 P物質 P物質受容体セロトニン GABA グリシン

项目摘要

1.The EM study in the rat medullary dorsal horn (MDH) suggested that substance P (SP) released from axon terminals might not only act on NK1 receptors facing SP-containing axon terminals but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act NK1 receptors at some distances from the synaptic sites.2.Co-existence of calcium-binding protein in neurons of the rat medullary dorsal horn was examined by double immunofluorescence histochernistry.3.Neuronal cell bodies in the mesencephalic nucleus of teigeminal nerve (MNT) of the rat were indicated to be in synaptic contact with GABAergic axon terminals and to express functional GABA_<A>receptors but not functional GABA_<B>receptors. Seretoninergic innervation of MNT neurons by the raphe nuclei were also observed.4.SP-containing primary afferent fibers were found to be in synaptic contact with GABAergic and glycinergic neurons in laminae I and II of the rat MDH.5.In slice perparations, correspondence between morphological features and electrical membrane properties of rat MDH neurons were examined in slice preparations by intracellular recording and biocytin-injection combined with histochemical and immunohistochemical staining techniques. The intrinsic MDH neurons were classified into 4 groups.6.Some neurons in the rat dorsal raphe nucleus (DR) were found to project simultaneously to the striatum and the raphe magnus nucleus/gigantocellular nucleus alpha region by axon collaterals. Correspondence between morphological features and electrical membrane propeties of DR neurons were also examined in slice perparations by intracellular recording and biocytin-injection combined with imrnunohistochemistry.7.It was indicated in the rat that protein kinase C gamma was expressed in many nociception-related MDH neurons.

1.在大鼠髓背角（MDH）的电镜研究表明，轴突末端释放的P物质（SP）不仅作用于面向含SP的轴突末端的NK1受体，而且在细胞外液中扩散到比突触间隙更大的距离，在距离突触位点一定距离处作用于NK1受体。采用双免疫荧光组织染色法观察大鼠髓背角神经元钙结合蛋白的共存。大鼠外源神经中脑核（MNT）神经元胞体与gaba能轴突末端突触接触，表达功能性GABA_<A>受体，而不表达功能性GABA_<B>受体。此外，还观察到中缝核对MNT神经元的血清素能支配。发现含sp的初级传入纤维与大鼠mdh I和II层的gaba能神经元和甘氨酸能神经元有突触接触。在切片制备中，采用细胞内记录和生物细胞素注射结合组织化学和免疫组织化学染色技术检测大鼠MDH神经元形态学特征与电膜特性的对应关系。内禀MDH神经元分为4组。发现大鼠中缝背核（DR）的部分神经元通过轴突侧枝同时投射到纹状体和中缝大核/巨细胞核α区。通过细胞内记录和生物细胞素注射结合免疫组织化学的方法，在切片制备中考察了DR神经元的形态特征与电膜特性的对应关系。结果表明，在大鼠中，蛋白激酶C γ在许多伤害感觉相关的MDH神经元中表达。