Neuronal circuitry in relation with transmitters and their receptors

与递质及其受体相关的神经回路

• 批准号: 08458245
• 负责人: MIZUNO Noboru
• 金额: $ 4.03万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458245/
• 关键词: GABA; Glycine; Glutamate receptor; Peptide; Trigeminal system; Cerebellum; Striatum; Olfactory system; セロトニン; P物質受容体; 三叉神経核; 痛覚; 嗅覚; オピオイド受容体; エンケファリン; 免疫組織化学; 逆行性標識法

1.By combining the retrograde fluorescence-labeling method with the immunofluorescent histochemistry for glutamic acid decarboxylase or glycine, the parvicellular medullary reticular formation including the juxtatrigeminal region were found to constitute one of the main interneuron pools for inhibitory (GABAergic and/or glycinergic) premotor neurons for facial and hypoglossal motoneurons in the rat.2.It was found immunohistochemically that mGluR2 and mGluR5 were expressed in two non-overlapping populations of Golgi cells in the cerebellum of the rat.3.By combining immunofluorescence and fluorescene histochemical techniques with the retrograde fluorescence-labeling method, axon terminals of serotoninergic neurons were found light and eletron microscopically to be in synaptic contact with nociceptive projection neurons in the caudal spinal trigeminal nucleus of the rat ; the nociceptive projection neurons were identified by retrograde labeling with the tracers injected into contralateral … More thalamus or the ipsilateral parabrachial nucleus and by expression of FOS after subcutaneous injection of formalin into the perioral regions or by synaptic contacts with axon terminals labeled with I-B4.4.Specific antibodies were produced against rat preprodynorphin, preproenkephalin, and preprotachykinin A.By using these antibodies, projection neurons in the striatum of the rat were divided into two groups ; the neurons of one group contained enkephalin and sent projection fibers to the globus pallidus, and those of the other group contained tachykinins and dynorphins/Leu-enkephalin and sent projection fibers to substantia nigra.5.The distributions of mGluR7a and mGluR7b were examined immunohistochemically in the rat and mouse by using antibodies raised against rat mGluR7a and human mGluR7b. The results indicated that mGluR7 might play roles not only as an autorecepter in glutamatergic axon terminals, but also as a presynaptic heteroreceptor in non-glutamatergic axon terminals.6.Light and electron microscopic immunohistochemical study in the rat indicated that glutamate release from axon terminals of projection fibers from the main and accessory olfactory bulbs might be regulated presynaptically and differentially through mGluR2/3, mGluR7a and/or mGluR8. Less

1.将逆行荧光标记法与谷氨酸脱羧酶或甘氨酸的免疫荧光组化相结合，发现大鼠面部和舌下运动神经元的抑制性（gaba能和/或甘氨酸能）前运动神经元的主要中间神经元池之一是包括近叉区在内的细胞旁髓网状结构。免疫组化发现mGluR2和mGluR5在大鼠小脑两个不重叠的高尔基细胞群中表达。采用免疫荧光和荧光组化技术结合逆行荧光标记法，光镜和电镜下发现大鼠三叉神经脊神经核尾侧5 -羟色胺能神经元轴突末端与伤害性投射神经元突触接触；通过向对侧丘脑或同侧臂旁核注射示踪剂逆行标记、口周区皮下注射福尔马林后FOS的表达或与标记为I-B4.4的轴突末梢的突触接触来识别伤害性投射神经元。制备了针对大鼠前驱肌啡肽、前驱脑啡肽和前驱脑啡肽a的特异性抗体，利用这些抗体将大鼠纹状体的投射神经元分为两组；4 .一组神经元含有脑啡肽，向苍白球发送投射纤维；另一组神经元含有快动素和运动啡肽/亮氨酸脑啡肽，向黑质发送投射纤维。采用免疫组化方法检测mGluR7a和mGluR7b在大鼠和小鼠体内的分布，分别采用抗大鼠mGluR7a和人mGluR7b抗体。结果表明，mGluR7不仅在谷氨酸能轴突末端作为自受体，而且在非谷氨酸能轴突末端作为突触前异受体发挥作用。大鼠的光镜和电镜免疫组化研究表明，从主嗅球和副嗅球投射纤维轴突末端释放谷氨酸可能通过mGluR2/3、mGluR7a和/或mGluR8在突触前进行差异调节。少

项目成果

Eiki Wada: "Metabotropic glutamate receptor subtypes in axon terminals of projection fibers from the main and accessory olfactory bullbs" J.Comp.Neurol.(in press).

Eiki Wada：“主嗅球和副嗅球投射纤维轴突末端的代谢型谷氨酸受体亚型”J.Comp.Neurol.（出版中）。

李 雲慶: "GABAergic and glycinergic neurons projicting to the trigeminal motor nucleus : a double labeling study in the rat." The Journal of Comparative Neurology. 373・4. 498-510 (1996)

李云清：“投射到三叉神经运动核的 GABA 能和甘氨酸神经元：大鼠的双重标记研究”，《比较神经学杂志》373・4（1996）。

根岸章夫: "Metabotropic glutamate receptor mG1uR2 and mG1uR5 are exoressed in two nonoverlaooing populations of Golgi cellsin the rat cerebellum" Neuroscience. 75・3. 815-826 (1996)

Akio Negishi：“代谢型谷氨酸受体 mG1uR2 和 mG1uR5 在大鼠小脑的两个非重叠高尔基体细胞群中外泌”《神经科学》75·3 (1996)。

李 金蓮: "Association of serotonin-like immunoreactives axons with nociceptive projection neurons in the caudal spinal trigeminal nucleus of the rat" The journal of Comparative Neurology. 384・1. 127-141 (1997)

Jinlian Lee：“大鼠尾椎三叉神经核中血清素样免疫反应性轴突与伤害性投射神经元的关联”比较神经学杂志 384・1（1997）。

李 金蓮: "Distribution of trigeminohypothalamic and spinohypothalamic tract neurons displaying substance P receptor-like immunoreactivity in the rat" The Journal of Compartive Neurology. 378・4. 508-521 (1997)

李金莲：“大鼠中显示 P 物质受体样免疫反应性的三叉下丘脑和脊髓下丘脑束神经元的分布”比较神经学杂志 378・4（1997）。