STUDIES ON THE DIFFERENTIATED FUNCTION AND GROWTH OF THYROID FOLLICULAR CELLS.

用人甲状腺和动物甲状腺研究了甲状腺滤泡细胞的生长和分化功能。功能正常的fisher大鼠甲状腺细胞（frtl-5）在egf结合域上存在异常，但利用特异性cdna探针和单克隆抗体证实其具有egf受体。从frtl-5细胞中获得了具有8-azaguanine抗性的突变克隆。这些被应用于与几个人类细胞的细胞融合研究。所获得的结果对观察分化功能的发展及其基因表达具有非常有趣的意义。研究了白细胞介素-1 α对小鼠甲状腺的影响。服用il-1 7天。甲状腺功能受到严重抑制。进一步。il-1给药3周后，甲状腺对TSH无反应，垂体TSH含量升高。采用人甲状腺细胞原代培养法，研究了egf受体的特性及egf和TSH的生长效应。癌细胞与egf的结合亲和力明显降低，但对egf反应良好。正常（非肿瘤性）和良性肿瘤细胞发现egf受体的结合亲和力与外源性egf结合生长刺激物3thymidine之间呈密切负相关。从未分化巨细胞甲状腺癌患者的肿瘤细胞中建立细胞系。这些细胞产生多种生长因子和细胞因子，如AD tgf - α、il-1 α、CSF和PTH样物质。它们的生长在很大程度上依赖于血清成分，并受各种生长因子的影响。综上所述，本研究获得了许多与甲状腺细胞生长和分化功能有关的新证据。其中大部分对今后的研究发展及在甲状腺研究中的临床应用具有一定的参考价值。

CELL GROWTH AND DIFFERENTIATED FUNCTIONS OF THE THYROID FOLLICULAR CELLS WERE STUDIED USING HUMAN AND ANIMAL THYROIDS.FUNCTIONING FISHER RAT THYROID CELLS (FRTL-5) WERE FOUND TO HAVE ABNORMALITY ON THE EGFBINDING DOMAIN, BUT THEIR POSSESSION OF EGF-RECEPTOR WAS PROVED USING SPECIFIC CDNA PROBES AND MONOCLONAL ANTIBODIES. MUTANT CLONES WITH 8-AZAGUANINE RESISTANCE WERE OBTAINED FROM THE FRTL-5 CELLS. THESE WERE APPLIED TO THE CELL-FUSION STUDY WITH SEVERAL HUMAN CELLS. RESULTS OBTAINED WERE OF VERY INTRIGUING TO SEE THE DEVELOPMENT OF DIFFERENTIATED FUNCTIONS AND THEIR GENE EXPRESSION.EFFECTS OF INTERLEUKIN-1 ALPHA ON THE MOUSE THYROID WERE STUDIED. DURING ADMINISTRATION OF IL-1 FOR 7 DAYS. THE THYROID FUNCTIONS WERE GREATLY SUPPRESSED. FURTHER. AFTER 3 WEEKS OF IL-1 ADMINISTRATION, THE THYROID ACQUIRED UNRESPONSIVENESS TO TSH AND PITUITARY TSH CONTENTS WERE INCREASED.USING PRIMARY CULTURE OF VARIOUS HUMAN THYROCYTES, PROPERTIES OF EGF RECEPTOR AND GROWTH EFFECTS OF EGF AND TSH WERE STUDIED. CANCER CELLS HAD SIGNIFICANTLY SMALLER BINDING AFFINITY BUT WELL RESPONDED TO EGF. NORMAL (NON-NEOPLASTIC) AND BENIGN TUMOR CELLS WERE FOUND TO SHOW A CLOSE NEGATIVE CORRELATION BETWEEN BINDING AFFINITY OF EGF RECEPTOR AND GROWTH STIMULATORY 3HTHYMIDINE INCORPORATION BY EXOGENOUS EGF.A CELL LINE WAS ESTABLISHED FROM TUMOR CELLS OF A PATIENT WITH UNDIFFERENTIATED GIANT CELL THYROID CANCER. THESE CELLS HAVE BEEN PRODUCING SEVERAL GROWTH FACTORS AND CYTOKINES SUCH AD TGF-ALPHA, IL-1 ALPHA, CSF AND PTH LIKE SUBSTANCE. GROWTH OF THEM IS GREATLY DEPENDENT ON SERUM COMPONENT AND IS ALSO AFFECTED BY VARIOUS GROWTH FACTORS.IN SUMMARY, QUITE A FEW NOVEL EVIDENCES CONCERNING WITH THE THYROID CELL GROWTH AND THEDIFFERENTIATED FUNCTIONS WERE OBTAINED. MOST OF THEM WILL SURELY BE USEFUL FOR THE FUTURE DEVELOPMENT OF THE RESEARCHES AND THEIR CLINICAL APPLICATIONS IN THE THYROID STUDIES.