Effect of Aging on the Structure and Sebum of Human Sebaceous Glands ; Morphological and Biochemical Study

衰老对人体皮脂腺结构和皮脂的影响；

• 批准号: 63480244
• 负责人: SATO Yoshio
• 金额: $ 1.92万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480244/
• 关键词: Aging; Sebaceous Gland; Computer-Stereography; Electron Microscopy; Sebum; Lipid Analysis; Urinary Androgen; ヒト脂腺形態; 尿中17-KS; 薄層クロマトグラフィ-; ガスクロマトグラフィ-; 脂腺活動性; 年令推移; 薄層クロマトグラフィー; ガスクロマトグラフィー; 年齢推移

To elucidate the changes of human sebaceous gland function with aging, forehead sebaceous glands of normal individuals of various ages were examined by computer-stereography and electron microscopy, and their forehead sebum samples were biochemically analyzed. The three-dimensional analysis of sebaceous glands using a computer graphic analysis system (Cosmozone 2SA, Nikon, Tokyo) was performed. In females, the volumes of sebaceous glands were small in subjects of less than 10 years of age, the largest at 20s and became smaller after 50s. In males, although they also reached the maximum at 20s,they maintained the large sizes until 40s or 50s and began to decrease at 60s. The larger average volumes of sebaceous glands showed the larger standard deviation values. The large sebaceous glands stereographically revealed balloon-like swelling. The increase in volume of sebaceous gland was due to increases in both number and size of sebocytes. Ultrastructurally, in the large sebaceous glands of … More young adults the peripheral sebocytes were cuboid in shape and the mature sebocytes had a large number of lipid droplets of a similar size, whereas in the small ones of children and aged persons the peripheral sebocytes were flat in shape and the mature sebocytes had lipid droplets of various sizes. These morphological and morphometrical data suggest that there are distinct age-related structural changes of human sebaceous glands in both sexes, although there is a difference in their size between the sexes in middle-aged individuals. Sebum samples were obtained from the forehead skin of normal human individuals of various ages by an absorption method. The amount of sebum rapidly increased until 20s in both sexes. It gradually decreased after 40s in females, whereas it was maintained at a high level after 40s in males. Wax esters/[cholesterol + cholesterol esters] (WE/[C+CE]) rations, which may be a good index to express the sebaceous gland activities, in the sebum samples, were measured by thin-layer chromatography. The age-related change of the ratios was similar to that of sebum amounts in females, but it was not in males. The ratios in males decreased after 40s similarly to those in females, although they kept higher levels in males than females. The WE fractions were extracted from the sebum samples and their fatty acid methyl esters were analyzed by gas-liquid chromatography using fused-silica capillary column. The percentages of C_<16:1> in WE also showed age-related changes, corresponding to those of WE/[C+CE] ratios, in both sexes. Urinary androgens were separated from 24 hrs-urine samples of all the individuals, trimethylsilylated and submitted to analysis by gas-liquid chromatography. The amounts of testosterone showed age-related changes positively correlated with those of either WE/[C+CE] ratios or C_<16:1> straight chain fatty acid proportions in sebum in both sexes. The amount of 17-ketosteroid also revealed a positive correlation with these sebum indices in females, but in males it did not show such a correlation except for infancy period. The activity of male sebaceous glands seemed to be controlled principally by testosterone as an intrinsic factor, whereas the female sebaceous glands seemed to be more sensitive to adrenal androgens than male ones. These morphological and biochemical findings may indicate that human sebaceous glands show distinct age-related changes of structures and functions in both sexes, although there are some differences in the patterns of the changes between the sexes especially in middle ages. Less

为探讨人体皮脂腺功能随年龄增长的变化规律，应用计算机体视学和电镜技术对不同年龄正常人前额皮脂腺进行了观察，并对其前额皮脂进行了生化分析。使用计算机图形分析系统（Cosmozone 2SA，Nikon，Tokyo）进行皮脂腺的三维分析。在女性中，小于10岁的受试者的皮脂腺体积较小，20岁时最大，50岁后变小。在男性中，虽然它们也在20岁时达到最大值，但它们一直保持到40或50岁，并在60岁时开始减少。皮脂腺的平均体积越大，标准差值越大。大的皮脂腺立体显示气球样肿胀。皮脂腺体积的增加是由于皮脂细胞的数量和大小增加。在超微结构上，在大的皮脂腺中， ...更多信息 青年人的外周皮脂腺细胞呈长方体形状，成熟皮脂腺细胞具有大量大小相似的脂滴，而儿童和老年人的小皮脂腺细胞的外周皮脂腺细胞呈扁平形状，成熟皮脂腺细胞具有各种大小的脂滴。这些形态学和形态测量学数据表明，有不同的年龄相关的结构变化的人类皮脂腺在两种性别，虽然有一个差异，在他们的大小之间的性别在中年人。通过吸收法从不同年龄的正常人个体的前额皮肤获得皮脂样品。皮脂量迅速增加，直到20年代在两种性别。女性在40岁以后逐渐下降，而男性在40岁以后维持在较高水平。采用薄层色谱法测定了皮脂样品中蜡酯/[胆固醇+胆固醇酯]（WE/[C+CE]）比值，该比值可能是反映皮脂腺活性的良好指标。女性的这一比值随年龄的变化与皮脂量的变化相似，而男性的这一比值随年龄的变化与皮脂量的变化不一致。40岁以后，男性的这一比率与女性相似地下降，但男性的这一比率仍高于女性。从皮脂样品中提取WE组分，并使用熔融石英毛细管柱进行气液色谱分析其脂肪酸甲酯。WE中C_的百分比<16:1>也显示出与年龄相关的变化，与男女WE/[C+CE]比值的变化相对应。从所有个体的24小时尿样中分离尿雄激素，三甲基硅烷化并通过气液色谱法进行分析。睾酮含量随年龄的变化与WE/[C+CE]比值和皮脂中C_<16:1>直链脂肪酸比例呈正相关。17-酮甾体的含量也显示了女性的这些皮脂指数呈正相关，但在男性中，它没有显示出这种相关性，除了婴儿期。男性皮脂腺的活性似乎主要由睾酮作为内在因素控制，而女性皮脂腺似乎比男性对肾上腺雄激素更敏感。这些形态学和生物化学的研究结果可能表明，人类皮脂腺的结构和功能的年龄相关的变化，在两性之间的变化，特别是在中年有一些差异。少

项目成果

Yamamoto,A.,Sarizawa,S.,Ito,M.and Sato,Y.: "Fatty acid composition of sebum wax esters and urinary androgen level in normal human individuals." J.of Dermatological Science. 1. 269-276 (1990)

Yamamoto,A.、Sarizawa,S.、Ito,M. 和 Sato,Y.：“正常人皮脂蜡酯的脂肪酸组成和尿液雄激素水平。”

Ayako Yamamoto,他: "Effect of aging on sebaceous gland activity and on the fatty acid composition of wax esters." J.Invest.Dermatol.89. 507-512 (1987)

Ayako Yamamoto 等人：“衰老对皮脂腺活性和蜡酯脂肪酸组成的影响”，J.Invest.Dermatol.89 (1987)。

佐藤良夫,他: "加齢と皮膚" 清至書院, 16 (1986)

Yoshio Sato 等人：《衰老与皮肤》Seishi Shoin，16 (1986)

池田 和人: "男性における脂腺の年齢による形態的変化" 日本皮膚科学会雑誌. 101.

Kazuto Ikeda：“男性皮脂腺与年龄相关的形态变化”，日本皮肤病学会杂志 101。

伊藤雅章: "皮膚付属器疾患" 日本皮膚科学会専門医委員会, 20 (1989)

伊藤正明：《皮肤附属器疾病》日本皮肤科协会专家委员会，20（1989）