Clinical application of bioartificial mucosa.

本研究的目的是构建一种新型的口腔人工黏膜修复方法，其研究结果总结如下：1)利用人活细胞构建10英寸成纤维细胞和17%胶原凝胶的生物人工粘膜。2)用气干法对培养细胞进行染色体检查，以确定其正常和安全。在几个月的培养时间内，所有培养的细胞都有正常的结果。3)通过透明电子显微镜观察基底膜，确定了生物人工粘膜的最佳移植时机。因此，最适宜的移植时机是上皮细胞培养后第4周。4)生物人工黏膜移植时，防止感染和干燥。5)根据上述结果，在名古屋大学附属医院施行了3例生物人工黏膜移植。在所有病例中，移植物都存活得很好。未观察到免疫排斥反应。

The purpose of this study was to construct the new method of oral mucosal repair with the bioartificial mucosa.The results of this research project through 1988 and 1989 were summarized as follows.1) The bioartificial mucosa using human living cells could be constructed with IX 10" fibroblasts and 17% collagen gel. The histological findings were very similar with normal human mucosa.2) The chromosome of cultured cells were checked by the air- dry- method to be confirmed their normality and safety. All cultured cells had normal findings within a few months culture periods.3) The most suitable timing of grafting of the bioartificial mucosa was decided to observed the basement membrane with transparent electron microscope. As the result the most suitable timing of grafting was the fourth week after the epithelial culturing.4) When the bioartificial mucosa was grafted, the protection from infection and drying. To prevent them the slow releasing antibiotics was applied locally.5) Based upon above results, 3 cases of bioartificial mucosal grafting were carried out at Nagoya University Hospital. In all cases the graft survived very well. No immunological rejection was observed.