Transplantation of cultured mucosal epithelium : an experimental and clinical study

由H.Green等人于1975年构建的培养上皮已被广泛认为是用于皮肤缺损移植的有用材料。在整形和重建外科领域，许多临床报道已经证明了培养的上皮细胞的用途，这些培养上皮细胞已经成功地培养出来。此外，已有多位作者报道了同种异体培养上皮移植的成功应用。近年来，也有一些研究探讨了口腔黏膜上皮细胞移植在颌面外科的临床应用价值。我们使用了之前冷冻并长期保存的培养的移植物。我们的部门已经探索了一种新的治疗方法，即保存自体和异体培养的上皮，并将其自体和异体移植到皮肤或粘膜缺损处。

Cultured epithelium fabricated by H.Green et al.in 1975 have been widely acknowledged to be useful materials for the transplantation on skin defects. In the field of plastic and reconstructive surgery, many clinical reports have demonstrated the utility of cultured epithelium which have been sucessfully cultured. Moreover, several authors have reported the application of cultured epithelial allografting successfully. Recently, some researches have also discussed about the clinical utility of cultured oral mucosal epithelium for grafting in maxillofacial surgery. We utilized cultured grafts that had been previously frozen and maintained at-145ﾟC for a long time.Our department has been investigated a new therapeutic modality, in which autologous and allogenous cultured epithelium are preserved and auto- and allo- grafted to skin or mucosal defects.