Experimental Study of Bioartificial Mucosa for Reconstruction of Oral Mucosa.

（1）生物人工Mucosa的宪法：准备一个生物人工mucosa纤维块从兔子口腔mucosa中分离出来，在胶原凝胶中培养和收获， 6 × 10个细胞/粒，至少10天。生物人工木瓜的生长条件为37 ° C，5%<CO_2>，15%MEM+脂肪血清。2）生物人工木瓜的移植：生物人工木瓜被移植到皮肤缺陷上，准备在同一动物的数量上。“生物人工大麻太弱了，不能被认为是完美的。”所以生物修饰物被用来修复移植物。移植的最重要点是为了防止感染，并使其干燥。为了避免这些麻烦，我们就把这种化妆品融入了抗生素中。移植手术在移植后被浸泡了7天，完全存活下来。没有免疫学的拒绝被观察到.（3）生物人工Mucosa的微观结构：生物人工Mucosa的微观结构是由透明电子显微镜特别是基础Lamina研究的。四个星期后，在培养Hemi-desmosome被观察到了外延细胞和中间细胞之间的边界。完整的底部Lamina结构在当时没有看到。

1) Constitution of Bioartificial Mucosa : To prepare a bioartificial mucosa fibroblasts were isolated from a rabbit oral mucosa, cultured and harvested in collagen gel, 6 x 10 cells/dish for at least 10 days. The culture condition was 37゜C, 5% <CO_2> , 15%MEM+fetal calf serum.2) Transplantation of Bioartificial Mucosa : The bioartificial mucosa was transplanted on the skin defect prepared on the dorsum of the same animal. The bioartificial mucosa was too weak to be sutured. So bioadhesive was used to fix the graft. The most important point in the manner of transplantation was to prevent the infection and to be dry. To prevent these trouble, we emplied the ointment containing the antibiotics. The graft was vasculized 7 days after transplantation and survived completely. No imunological rejection was observed.3) Microstructure of Bioartificial Mucosa : The microstructure of the bioartificial mucosa was studied by transparent erectoron microscop especially basal lamina. Four weeks after culturing hemi-desmosome was observed in the border between epithelial cells and mesenchymal cells. However, complete structure of basal lamina was no seen at that time.