Regulatory Mechanism of Genetic Regioo (hrp) Controls Both Plant Pathogenicity and Hyper-sensitive Reaction.

遗传区域（hrp）的调控机制控制植物致病性和超敏反应。

• 批准号: 04660045
• 负责人: TSUYUMU Shinji
• 金额: $ 1.41万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660045/
• 关键词: Citrus canker; hrp; Glucan; Hyper-sensitive reaction; RFLP; Xanthomonas; Chemiluminescens; Elicitor; Suppressor; 病原性遺伝子; グルカン; ポーリン

1. From the analyzes of non-pathogenic mutants of Xanthomonas campestris pv.citri induced by Tn5 trasposon tagging and of cosmid clones, it became apparent that this pathogen has two different hrp regions ; one (hrpl) consists of relatively small region and other (hrp2) consists of 25kb region containing multiple hrp genes. 2. hrpl showed homology with the gene for glucosyl-transferase of Escherichia coli, and it was considered to be involved in glucan synthesis. Furthermore, glucan of this pathogen was cyclic glucan similar to the one of Agrobacterium tumefascines and Rhizobiaceae. This indicates that this pathogen may require the adsorption to plant cells by glucan for the inductions of pathogenicity and hypersensitive reaction. 3. hrp2 hybridized with hrp of Pseudomonas solanacearum.hrp2 was turned out to be a good probe for RFLP analysis to identify species and pathovars of Xanthomonas. The clone which complements F8 could be obtained. 4. Using gene fusion with phoA, it was suggested that the induction of hrp2 requires the exposure to plant cells. 5. Since the importance of early reaction was thought to be important, the chemiluminescence due to the formation of free radicals was investigated. From this study, it was found that this pathogen produces both heat-stable elicitor and suppressor.

1.通过Tn5转座子标记法诱导柑橘黄单胞菌致病变种的非致病突变体和粘粒克隆的分析，发现该病原菌有两个不同的hrp区，一个（hrp 1）由相对较小的区域组成，另一个（hrp 2）由含有多个hrp基因的25kb区域组成。2. hrpl基因与大肠杆菌葡糖基转移酶基因同源，推测其可能参与葡聚糖的合成。此外，该病原菌的葡聚糖与根癌农杆菌和根瘤菌科的葡聚糖相似，为环状葡聚糖。这表明该病原菌可能需要葡聚糖吸附在植物细胞上才能诱导致病性和过敏反应。3. hrp2与青枯假单胞菌的hrp进行杂交，结果表明hrp2是一个很好的用于黄单胞菌属种和致病变种的RFLP分析的探针。可获得与F8互补的克隆。4.使用与phoA的基因融合，表明hrp2的诱导需要暴露于植物细胞。5.由于早期反应的重要性被认为是重要的，因此研究了由于自由基的形成而产生的化学发光。从这项研究中，发现该病原菌产生热稳定的激发子和抑制子。

项目成果

Gold, S., S.Nishio, S.Tsuyumu and N.T.Keen: "Analysis of the pelE promoter in Erwinia chrysanthemi." Mole.Plant-Microbe Interact. 5. 170-178 (1992)

Gold, S.、S.Nishio、S.Tsuyumu 和 N.T.Keen：“菊欧文氏菌中 pelE 启动子的分析”。

Jhoncon,J.: "Determination of nucleotide sequence of a region producing multiple polygalacturonascs from Ercoinia carotovora sulsp.carotovora" Aun.Phytopath.Soc.Japan. 60(2) (印刷中). (1994)

Jhoncon，J.：“来自胡萝卜软腐菌的多聚半乳糖醛酸区域的核苷酸序列的测定”Aun.Phytopath.Soc.Japan 60(2)（出版中）。

Tsuyumu, S., K.Tanaka, N.Furutani, Y.Takikawa and C.Boucher: "Genes involved in pathogenicity of Xanthomonas campestris pv.citri." Proc.Internat.Conf.Plant Pathogenic Bacteria. 8 : in press.

Tsuyumu, S.、K.Tanaka、N.Furutani、Y.Takikawa 和 C.Boucher：“参与野油菜黄单胞菌柑橘病原菌致病性的基因”。

Takikawa, Y.N.Nishiyama, K.Ohata, S.Tsuyumu and M.Goto: "Synonymy of Pseudomonas syringae pv.maculicola and Pseudomonas syringae pv.tomato" Proc.Internat.Conf.Plant Pathogenic Bacteria. 8 : in press.

Takikawa、Y.N.Nishiyama、K.Ohata、S.Tsuyumu 和 M.Goto：“丁香假单胞菌 pv.maculicola 和假单胞菌丁香 pv.tomato 的同义词”Proc.Internat.Conf.Plant 病原细菌。

Tsuyumu, S., M.Watanabe, K.Abe, S.Watabe, S.Arai, M.Goto and Y.Takikawa: "Simple and sensitive method for the detection of Escherichia coli in natural environments using ice nucleation active gene." Biosci.Biotech.Biochem.56. 1867-1868 (1992)

Tsuyumu, S., M.Watanabe, K.Abe, S.Watabe, S.Arai, M.Goto 和 Y.Takikawa：“利用冰核活性基因在自然环境中检测大肠杆菌的简单而灵敏的方法。”