Increase in intracellular Ca^<2+> and neuronal injury

细胞内Ca^2增加和神经元损伤

• 批准号: 04670874
• 负责人: ARAI Hajime
• 金额: $ 1.41万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670874/
• 关键词: Hippocampus; Neuron; Glutamate; Calcium; Cell death; Hypothermia; 神経毒性; 神経保護; 培養神経細胞; グリア; 神経保護作用; 脳虚血

To elucidate the precise mechanism of neuronal protection by brain hypothermia, we conducted an investigation as to what effect low temperature had on glutamate-induced intracellular free calcium ([Ca^<2+>]i) accumulation and cell death in cultured hippocampal neurons. Fifteen minutes of exposure to either 100muM or 1mM glutamate uniformly induced a marked increase in [Ca^<2+>]i, with delayd recovery and massive neuronal death under the conditions of both 37ﾟC and 30ﾟC.The study indicated that brain hypothermia cannot save neurons once glutamate is released during ischemia, and that intraischemic hypothermia in vivo probably prevents the development of ischemic neuronal injuly by suppressing extracellular glutamate release.

为了阐明脑低温对神经元保护的确切机制，我们进行了一项研究，探讨低温对谷氨酸诱导的海马神经元细胞内游离钙（[Ca^2+]i）积累和细胞死亡的影响。在37 ℃和30 ℃条件下，暴露于100 μ M或1mM谷氨酸15分钟均诱导[Ca^2+]i显著增加，恢复延迟，大量神经元死亡。研究表明，一旦缺血期间谷氨酸释放，脑低温不能挽救神经元，体内缺血时低温可能通过抑制细胞外谷氨酸释放而阻止缺血神经元的发育。

项目成果

新井一: "Effect of low temperature on glutamate-inducedintracellular calcium accumulation and cell dcath in cultured hippocampal neurons" NeuroscienceLetters. 163. 132-134 (1993)

Hajime Arai：“低温对培养海马神经元中谷氨酸诱导的细胞内钙积累和细胞死亡的影响”《神经科学快报》163. 132-134 (1993)。

Arai, Hajime: "Effect of low temperature on glutamate-induced intracellular calcium accumulation and cell death in cultured hippocampal neurons" Neurosci Lett. 163. 132-134 (1993)

Arai, Hajime：“低温对培养海马神经元中谷氨酸诱导的细胞内钙积累和细胞死亡的影响”Neurosci Lett。

Arai,Hajime: "Effect of low temperature on glutamate-induced intracellular calcium accumulation and cell death in cultured hippocampal neurons" Neurosci Lett. 163. 132-134 (1993)

Arai，Hajime：“低温对培养海马神经元中谷氨酸诱导的细胞内钙积累和细胞死亡的影响”Neurosci Lett。

新井一: "低脳温はグルタミン酸誘発Ca2^+細胞内流入と神経細胞死を抑制するか?" Brain Hypoxia. 8. 71-75 (1994)

Hajime Arai：“低脑温度会抑制谷氨酸诱导的 Ca2+ 细胞内流入和神经元细胞死亡吗？” 8. 71-75 (1994)

新井 一: "グルタミン酸による神経細胞障害と細胞内カルシウム濃度" Brain Hypoxia. 6. 23-29 (1992)

Hajime Arai：“谷氨酸诱导的神经元损伤和细胞内钙浓度”脑缺氧。 6. 23-29 (1992)