Amniotic epithelial cells transplantation in the ischemic brain

羊膜上皮细胞移植用于缺血性脑组织

• 批准号: 13671466
• 负责人: ARAI Hajime
• 金额: $ 1.22万
• 依托单位: JUNTENDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671466/
• 关键词: Amniotic epitherial cell; ischemic brain; transplantation; 神経細胞; 神経再生; 神経幹細胞

We investigated the potential use of rat amniotic epitherial cells as donor cells for transplantation-based therapy in brain ischemia.Methods are listed as below; primary culture of amniothic epitherial cells; immunocytochmical staining of amniotic epitherial cells; execution of ischemic brain(infarction)model rat; genic induction of GFP into the amniotic epitherial cells; and transplantation of amniotic epitherial cells into the model rat brain.Results: The amniotic epitherial cells are collected abundantly in E17 rats and the most lasting. On immunocytochmical staining, the rat amniotic epitherial cells were positive reaction to neuronal markers, micro-tubule-associated protein 2, neurofilament and nestin. The induction of retroviral vector enhanced by green fluorescent protein(GFP) succeeded. The grafted amniotic epitherial cells revealed the migration into the brain(CA1 pyramidal layer) and the existence of that cells was confirmed.Conclusion: these results suggest that intracerebral transplantation of amniotic epitherial cells may have therapeutic potential for the treatment of ischemic damage in neuronal disorders.

我们研究了大鼠羊膜上皮细胞作为供体细胞用于脑缺血移植治疗的可能性。方法如下：羊膜上皮细胞原代培养；羊膜上皮细胞免疫细胞化学染色；缺血性脑（梗死）模型大鼠的处死；基因诱导GFP进入羊膜上皮细胞；羊膜上皮细胞移植模型大鼠脑。结果：E17大鼠羊膜上皮细胞数量丰富且保存时间最长。免疫细胞化学染色显示，大鼠羊膜上皮细胞对神经元标志物、微管相关蛋白2、神经丝和巢蛋白均呈阳性反应。绿色荧光蛋白（GFP）增强逆转录病毒载体的诱导成功。移植的羊膜上皮细胞向脑组织（CA1锥体层）迁移，证实了该细胞的存在。结论：羊膜上皮细胞脑内移植治疗缺血性损伤具有一定的治疗潜力。

项目成果

Hidenori Okawa: "Amniotic epitherial cells transform into neuron-like cells in the ischemic brain"NEUROREPORT. Vol 12,No 18. 2004-2007 (2001)

Hidenori Okawa：“羊膜上皮细胞在缺血性大脑中转化为神经元样细胞”NEUROREPORT。

Hidenori Okawa, Osamu Okuda: "Amniotic epitherial cells transform into neuron-like cells in the ischemic brain."NEUROREPORT. Vol12・No18. 4003-4007 (2001)

Hidenori Okawa、Osamu Okuda：“羊膜上皮细胞在缺血性脑中转化为神经元样细胞。”NEUROREPORT 第 12 卷·第 4003-4007 期（2001 年）。

Okawa H., Okuda O., Arai H. et al.: "Amniotic epithelial cells transform into neuron-like cells in theischemic brain"Neuroreport. 12(18). 4003-4007 (2001)

Okawa H.、Okuda O.、Arai H. 等人：“羊膜上皮细胞在缺血脑中转化为神经元样细胞”Neuroreport。