MOLECULAR ANALYSIS OF GASTRIC CANCER WITH CONSIDERATION OF HELICOBACTER PYLORI AND EPSTEIN-BARR VIRUS INFECTION

考虑幽门螺杆菌和 Epstein-Barr 病毒感染的胃癌分子分析

• 批准号: 10670463
• 负责人: ARAI Hajime
• 金额: $ 1.98万
• 依托单位: HAMAMATU UNIVERSITY SCHOOL OF MEDECINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670463/
• 关键词: GASTRIC CANCER; HELICOBACTER PYLORI; EPSTEIN-BARR VIRUS; MICROSATELLITE INSTABILITY; p53点突然変異; 遺伝子不安定性

Recently, microsatellite instability (MI) detected by PCR spanning CA repeats has been reported in various type of cancers. As concerning as gastric carcinoma, many studies reported that MI were found in 18-38%. But there are few reports about MI and loss of heterozygosity in gastric carcinoma, with or without H.pylori infection. We therefore attempted to elucidate the relationship between H.pylori infection and MI. We have examined 124 cases of gastric carcinomas in our hospitals. The status of Helicobacter pylori (H.pylori) infection was investigated in all cases. Only 6 cases (4.9%) belonged to H.pylori negative gastric carcinomas. Seven microsatellite loci were analyzed in available 61 cases. MI was correlated with neither age and sex of the patients nor H.pylori infection. H.pylori negative gastric cancers was not stained with Epstein-Barr virus encoded small RNAs in situ hybridization. In regard to loss of heterozygosity in same loci, we have gotten the same result with MI. Our data show that very few cases of Japanese gastric carcinoma are considered to be negative for H.pylori infection, and H.pylori infection seems to be independent of MI and LOH in gastric carcinogenesis.

近年来，通过跨CA重复序列的PCR检测到的微卫星不稳定性（MI）已在各种类型的癌症中被报道。与胃癌一样，许多研究报道MI发生率为18- 38%。但在胃癌中，无论是否有H.pylori感染，MI和杂合性缺失的研究报道很少。因此，我们试图阐明幽门螺杆菌感染和心肌梗死之间的关系。我们检查了124例胃癌在我们的医院。对所有病例进行幽门螺杆菌（H.pylori）感染状况调查。H.pylori阴性胃癌6例（4.9%）。对61例患者的7个微卫星位点进行了分析。MI与年龄、性别及H.pylori感染无关。幽门螺杆菌阴性的胃癌细胞不被EB病毒编码的小RNA原位杂交染色。对于同一位点的杂合性丢失，我们用MI得到了相同的结果。我们的数据表明，日本胃癌中很少有幽门螺杆菌感染阴性的病例，幽门螺杆菌感染似乎与胃癌发生中的MI和洛无关。

项目成果

Hajime Arai: "Microsattellite instability and loss of heterozygosity in Japanese gastric caroinoma with consideration of Helicobacter pylori infection"3rd international gastric cancer congress. (1999)

Hajime Arai：“考虑幽门螺杆菌感染的日本胃癌中的微卫星不稳定性和杂合性丢失”第三届国际胃癌大会。

H. Arai, S. Yoshii, Y. Maruyama, T. Furuta, E. Kaneko, H. Hanai, H. Sugimura, K, Sugimoto, S. Honda, F. Watanabe, Y. Kitayama, K. Koda: "Microsatellite instability and loss of heterozygosity in Japanese gastric carcinoma with consideration of Helicobacter

H. Arai、S. Yoshii、Y. Maruyama、T. Furuta、E. Kaneko、H. Hanai、H. Sugimura、K、Sugimoto、S. Honda、F. Watanabe、Y. Kitayama、K. Koda：“微卫星

Hajime Arai: "Microsatellite instability and loss of heterozygosity in Japanese gaotric carcinoma with consideration of Helicobacter pylori infection"3rd international gastric cancer congress. (1999)

Hajime Arai：“考虑幽门螺杆菌感染的日本胃癌中的微卫星不稳定性和杂合性丢失”第三届国际胃癌大会。