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Amniotic epithelial cell transplantation to adult rat brain

羊膜上皮细胞移植至成年大鼠脑部

基本信息

批准号：
10671319
负责人：
ARAI Hajime
金额：
$ 1.92万
依托单位：
Juntendo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Neurons and glial cells are thought be generated during development of the central nervous system (CNS) from neuronal or glial progenitorcells. These progenitor cels originate from embryonal stem (ES) celts, which are capable of selfrenewal and can generate alL of the cell types. Aminotic epithelial cells also stem from the same origin as ES celts, or the so-called "inner cell mass". The purpose of this study is to examine the characteristics of rat amniotic epithelial (RAE) cells and evaluate whether these cells have a potential as donor cells for neuronal transplantation therapy. We have established a primary culture of RAE cells from placental materiaLs of Wistar rats. Rae cells, originally roundshaped cells, morphologically changed into spindle-shaped neuron-like cells with a few nurite-like processes in B27-supplemented Neurobasal medium (Neurobasal/B27). Cultured RAE cells in Neurobasal/B27 stopped cell diversion and also showed several immunoreactivities such as neuronal and progenitor cell markers. Transplanted into normal rat brains, RAE cells survivied for more than three months without immunosuppressants and also express mainly neuronal markers on their surfaces in vivo. These results indicate that RAE cells cultured in Neurobasal/B27 may have a potential as donor cells in transplantation therapy for CNS disorders.

神经元和神经胶质细胞被认为是在中枢神经系统（CNS）发育过程中由神经元或神经胶质祖细胞产生的。这些祖细胞源自胚胎干（ES）细胞，它们能够自我更新并可以产生所有细胞类型。氨基上皮细胞也源自与ES细胞相同的起源，或所谓的“内细胞团”。本研究的目的是检查大鼠羊膜上皮（RAE）细胞的特征，并评估这些细胞是否具有作为神经元移植治疗的供体细胞的潜力。我们已经从 Wistar 大鼠的胎盘材料中建立了 RAE 细胞的原代培养物。 Rae 细胞最初为圆形细胞，在补充 B27 的 Neurobasal 培养基 (Neurobasal/B27) 中形态发生转变为纺锤形神经元样细胞，具有一些类似神经突起的突起。在 Neurobasal/B27 中培养的 RAE 细胞停止了细胞转移，并且还显示出多种免疫反应性，例如神经元和祖细胞标记。移植到正常大鼠大脑中后，RAE细胞在没有免疫抑制剂的情况下存活了三个月以上，并且在体内主要在其表面表达神经元标记。这些结果表明，在 Neurobasal/B27 中培养的 RAE 细胞可能具有作为中枢神经系统疾病移植治疗中供体细胞的潜力。