Detection of chromosome abnomality by fluorescent in situ hybridization in brain tumors.

通过荧光原位杂交检测脑肿瘤中的染色体异常。

• 批准号: 04670877
• 负责人: KAWAMOTO Keiji
• 金额: $ 1.34万
• 依托单位: KANSAI MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670877/
• 关键词: FISH; Brain Tumor; flow cytometry; chromosome; DNA-histogram; paraffin; Chromosome; brain tumor

(1)Flow cytometric analysis of the cell cycle in brain tumors.Flow cytometory (FCM) has been an effective method to abtain the biological aspects of brain tumors. We can clarify the mechanism of anticancer agents such as 1-hexycarbamoy 1-5 fluouracil, CDDP, MCNU and TNF.(2)Expression of oncogene products in brain tumors.In established brain tumor cells strains, immunohistochemical and flow cytometric methods are useful for evaluation of the positivity of c-myc products expression.(3)Development of FISH method for detection of chromosome abnomality.1)Chromosome numbers of 7,9,10,17 were dectected in glioma cell lines by FISH method.2)Chromosome numbers of 7,17 were high in gliomas obtained by surgical operation.3)Meningioma showed low chromosome numbers of 22, but high chromosome numbers of 22 in recurrent cases.(4)Detection of chromosome abnomality with use of paraffin embeded specimen.We succeeded to detect the chromosome numbers with use of paraffin blocks, which was correlated with fresh specimen.

(1)流式细胞术分析脑肿瘤细胞周期。流式细胞术（FCM）已成为获得脑肿瘤生物学特征的一种有效方法。阐明1-己碳氧基- 1-5氟尿嘧啶、CDDP、MCNU、TNF等抗癌药物的作用机制。(2)癌基因产物在脑肿瘤中的表达。在已建立的脑肿瘤细胞株中，免疫组织化学和流式细胞术方法可用于评估c-myc产物表达的阳性。(3)染色体异常检测FISH方法的发展。1)用FISH法检测胶质瘤细胞系的7、9、10、17号染色体数目。2)手术获得的胶质瘤染色体数目为7、17。3)脑膜瘤的染色体数目低，为22，复发病例的染色体数目高，为22。(4)利用石蜡包埋标本检测染色体异常。我们成功地用石蜡块检测了染色体数目，并与新鲜标本进行了相关。

项目成果

今堀 巧,河本 圭司: "PCNAの基礎的検討-FCMによる検出と細胞同期との関連" Oncology の進歩. 2. 58-64 (1992)

Takumi Imahori、Keiji Kawamoto：“PCNA 的基础研究 - FCM 检测与细胞同步之间的关系”《肿瘤学进展》2. 58-64 (1992)。

"ANALYSIS OF DNA PLOIDY PAT-TERN IN BRAIN TUMORS" Neuroimmunological Research. 5. 178-183 (1992)

“脑肿瘤中 DNA 倍体模式的分析”神经免疫学研究。

"Detection of chromosomal numerical aberration in glioma by FISH(fluorescence in situ hybridization)" HUMAN CELL. 6(1). 62-65 (1993)

“通过 FISH（荧光原位杂交）检测神经胶质瘤中的染色体数值畸变”人类细胞。

Sakai Kawamoto: "Flow cytometry による脳腫瘍におけるc-myc遺伝子産物と核DNA量の同時定量的解析" cytometry Research. 3. (1993)

Sakai Kawamoto：“通过流式细胞术同时定量分析脑肿瘤中的c-myc基因产物和核DNA含量”细胞计数研究3。（1993）。

笠井治文、今堀巧、河本圭司: "FISH法によるglioma染色体異常の検出" HUMAN CELL. 6. 62-65 (1993)

Harufumi Kasai、Takumi Imahori、Keiji Kawamoto：“通过 FISH 方法检测神经胶质瘤染色体异常” HUMAN CELL。 6. 62-65 (1993)