The efffect of irradiation on brain microvascular eudotherial cells

血管内皮细胞可以产生组织型纤溶酶原激活剂（t-PA）和纤溶酶原激活剂抑制剂1（PAI-1）。然而，脑微血管内皮细胞（BMEC）对这些物质的释放动力学尚不清楚。将猪脑经酶解和percoll梯度分离制备的BMEC暴露于含血栓（1.0IU/ml）、组胺（10mumol/ml）和tnf - α（500、1000、2000U/ml）的培养基中。在相应时间点获得的上清液进行t-PA、PAI-1和t-PA- PAI-1复合物的ELISA检测。在相应的时间点，这些物质的值明显高于对照组。这也证明了BMEC的t-PA和PAI-1的产量是平行增加的。目前的研究表明，在一定刺激下培养的BMEC的t-PA产量增加，然而，它伴随着PAl-1的产生。采用我们开发的方法和简化的方法，从猪肝细胞中提取脑微血管内皮细胞进行培养。将培养的细胞增加辐照剂量，用量热法测定细胞活力。SOGy剂量使增殖细胞的生存能力下降。半融合细胞在1000Gy照射24小时后开始产生辐照效应，在800Gy照射48小时后开始产生辐照效应，超过1000Gy照射对融合细胞也没有影响。增殖期和半融合期出现核碎裂的凋亡细胞。这些数据表明，Gamin射线对脑微血管内皮细胞在增殖期是有效的，但这些内皮细胞在非增殖期表现出强烈的抗性。结论经多次高剂量照射后，新形成的血管受到动静脉畸形和脑血管病等刺激的影响，而正常的脑血管没有受到大剂量的损伤。少

Vascular endothellal cells are known to produce tissue plasminogen activator(t-PA) and plasminogen activator inhibitor 1(PAI-1). However, the release kinetics of these substances by brain microvascular endothelial cells (BMEC) is less well known. BMEC prepared from porcine brains using enzymatic digestion and percoll gradient separation was exposed to culture medium containing thrombiri (1.0IU/ml), histamine (10mumol/ml) and TNF-alpha(500, 1000, 2000U/ml). The supernatant obtained at respective time points was subjected to ELISA for t-PA, PAI-1 and t-PA-PAI- 1 complex. The value of these substances were significantly higher than those of the controls at respective time points. It also proved that t-PA and PAI-1 production by BMEC increases in a parallel fashion. The present study suggests that t-PA production by BMEC cultured under given stimulation increases, however, it is accompanied by concomittant PAl-1 production.The brain inicrovascular endothereal cells were obtained from porci … More ne and were cultured by using our developed and simplified methods. These cultured cells were irradiated following with increasing doses and viability was examined with calorimetric assay methods. Proliferating cells showed the decrease of viability from the dose of SOGy. Semi confluent cells revealed the irradiation effct began 24hours later at 1000Gy, and 48hours later at 800Gy, but confluent cells were not effected even if over 1000Gy irradiation. Apoptotic cells showing the nuclear fragmentation were appeared at the proliferating and semi confluent stages. These data revealedthat Gamin ray was effective on the brain inicrovascular endotherial cells at proliferating stage, but these endotherial cells were strongly resistance at the non proliferating stage. Conclusion was introduced that new developed vessels were effected by irration such as the arteriovenous malfomation and brain desease of vascular tijmors, but normal brain vessels were not so injured following by hvdose of ceveral irradiation. Less