The efffect of irradiation on brain microvascular eudotherial cells

血管内皮细胞产生组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制物-1(PAI-1)。然而，脑微血管内皮细胞(BMEC)释放这些物质的动力学还不是很清楚。用酶消化法和Percoll梯度分离法从猪脑中分离出BMEC，将其置于含有血栓(1.0IU/ml)、组胺(10mumol/ml)和肿瘤坏死因子-α(500、1000、2000U/ml)的培养液中。取各时间点的培养上清液，进行t-PA、PAI-1和t-PA-PAI-1复合体的酶联免疫吸附试验。在各个时间点，这些物质的值均显著高于对照组。也证明了BMEC产生的t-PA和PAI-1是平行增加的。本研究提示，在一定刺激条件下培养的人脐静脉内皮细胞产生t-PA增加，但伴随PAL-1的产生。脑微血管内皮细胞取自Porci…用我们改进和简化的方法培养了更多的Ne和Ne。对培养的细胞进行剂量递增的照射，并用量热法检测细胞存活率。随着SOGY剂量的增加，增殖细胞存活率下降。半融合细胞在1000Gy24小时后开始照射，800Gy48h后开始照射，但1000Gy以上照射对融合细胞无影响。增殖期和半融合期出现核碎裂的凋亡细胞。结果表明，伽玛射线对处于增殖期的微血管内皮细胞有明显的脑保护作用，而对非增殖期的微血管内皮细胞有较强的抵抗力。结论大剂量照射对新生血管有影响，但对正常脑血管无明显损伤。较少

Vascular endothellal cells are known to produce tissue plasminogen activator(t-PA) and plasminogen activator inhibitor 1(PAI-1). However, the release kinetics of these substances by brain microvascular endothelial cells (BMEC) is less well known. BMEC prepared from porcine brains using enzymatic digestion and percoll gradient separation was exposed to culture medium containing thrombiri (1.0IU/ml), histamine (10mumol/ml) and TNF-alpha(500, 1000, 2000U/ml). The supernatant obtained at respective time points was subjected to ELISA for t-PA, PAI-1 and t-PA-PAI- 1 complex. The value of these substances were significantly higher than those of the controls at respective time points. It also proved that t-PA and PAI-1 production by BMEC increases in a parallel fashion. The present study suggests that t-PA production by BMEC cultured under given stimulation increases, however, it is accompanied by concomittant PAl-1 production.The brain inicrovascular endothereal cells were obtained from porci … More ne and were cultured by using our developed and simplified methods. These cultured cells were irradiated following with increasing doses and viability was examined with calorimetric assay methods. Proliferating cells showed the decrease of viability from the dose of SOGy. Semi confluent cells revealed the irradiation effct began 24hours later at 1000Gy, and 48hours later at 800Gy, but confluent cells were not effected even if over 1000Gy irradiation. Apoptotic cells showing the nuclear fragmentation were appeared at the proliferating and semi confluent stages. These data revealedthat Gamin ray was effective on the brain inicrovascular endotherial cells at proliferating stage, but these endotherial cells were strongly resistance at the non proliferating stage. Conclusion was introduced that new developed vessels were effected by irration such as the arteriovenous malfomation and brain desease of vascular tijmors, but normal brain vessels were not so injured following by hvdose of ceveral irradiation. Less