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The efffect of irradiation on brain microvascular eudotherial cells

辐射对脑微血管真兽细胞的影响

基本信息

批准号：
09671456
负责人：
KAWAMOTO Keiji
金额：
$ 1.98万
依托单位：
Kansai Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671456/
关键词：
endotherial cell gamma-ray apoptosis tPAI irradiation

项目摘要

Vascular endothellal cells are known to produce tissue plasminogen activator(t-PA) and plasminogen activator inhibitor 1(PAI-1). However, the release kinetics of these substances by brain microvascular endothelial cells (BMEC) is less well known. BMEC prepared from porcine brains using enzymatic digestion and percoll gradient separation was exposed to culture medium containing thrombiri (1.0IU/ml), histamine (10mumol/ml) and TNF-alpha(500, 1000, 2000U/ml). The supernatant obtained at respective time points was subjected to ELISA for t-PA, PAI-1 and t-PA-PAI- 1 complex. The value of these substances were significantly higher than those of the controls at respective time points. It also proved that t-PA and PAI-1 production by BMEC increases in a parallel fashion. The present study suggests that t-PA production by BMEC cultured under given stimulation increases, however, it is accompanied by concomittant PAl-1 production.The brain inicrovascular endothereal cells were obtained from porci … More ne and were cultured by using our developed and simplified methods. These cultured cells were irradiated following with increasing doses and viability was examined with calorimetric assay methods. Proliferating cells showed the decrease of viability from the dose of SOGy. Semi confluent cells revealed the irradiation effct began 24hours later at 1000Gy, and 48hours later at 800Gy, but confluent cells were not effected even if over 1000Gy irradiation. Apoptotic cells showing the nuclear fragmentation were appeared at the proliferating and semi confluent stages. These data revealedthat Gamin ray was effective on the brain inicrovascular endotherial cells at proliferating stage, but these endotherial cells were strongly resistance at the non proliferating stage. Conclusion was introduced that new developed vessels were effected by irration such as the arteriovenous malfomation and brain desease of vascular tijmors, but normal brain vessels were not so injured following by hvdose of ceveral irradiation. Less

已知血管内皮细胞产生组织纤溶酶原激活物（t-PA）和纤溶酶原激活物抑制剂1（派-1）。然而，脑微血管内皮细胞（BMEC）释放这些物质的动力学是鲜为人知的。采用酶消化和梯度分离法从猪脑中制备BMEC，将BMEC暴露于含有凝血酶（1.0IU/ml）、组胺（10 μ mol/ml）和TNF-α（500、1000、2000 U/ml）的培养基中。对在各个时间点获得的上清液进行t-PA、派-1和t-PA-派- 1复合物的ELISA。这些物质的值在各个时间点均显著高于对照品。同时也证明了BMEC产生t-PA和派-1的能力以平行的方式增加。本研究表明，在一定的刺激条件下培养的BMEC产生t-PA的能力增加，但同时也伴随着PAI-1的产生。 ...更多信息新的和培养通过使用我们的发展和简化的方法。这些培养的细胞进行照射后，增加剂量和活力进行了检查与量热测定方法。体外培养的细胞在SOGy剂量下存活率下降。半汇合细胞显示1000 Gy照射24小时后开始产生效应，800 Gy照射48小时后开始产生效应，而汇合细胞即使在1000 Gy以上照射也不受影响。增殖期和半融合期出现凋亡细胞，核碎裂。提示γ射线对处于增殖期的脑微血管内皮细胞有一定的杀伤作用，而非增殖期的脑微血管内皮细胞则有较强的抵抗作用。结论：大剂量脑照射后，新生血管受到脑血管畸形、脑血管病等不合理因素的影响，而正常脑血管无明显损伤。少