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Study on Antineoplastic Effects of NK Cells and IL-2 for Malignant Brain Tumors.

NK细胞和IL-2对恶性脑肿瘤的抗肿瘤作用研究。

基本信息

批准号：
60570683
负责人：
KAWAMOTO Keiji
金额：
$ 0.26万
依托单位：
KANSAI MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570683/
关键词：
NK Cells NK Activities IL-2 Flow Cytometry Brain Tumor 抗腫瘍効果

项目摘要

The following are the summary of the above said study. The achievements made are five books, 19 papers, and 49 presentations at medical meetings.1. Cell kinetics of the NK cells and NK activities and T cell subsets of brain tumor patients. In clinical cases of brain tumors, pre- and postoperative T cell subsets and NK cells were measured flow cytometrically by using monoclonal antibodies, and their possibility acting as the indices to evaluate the immunological capacity of these patients were proven. NK activities were also acting an important role as the index.2. Evaluation of the antineoplastic effects by antineoplastic agents and interferon. A method of in vitro evaluation of the antineoplastic effects was developed using flow cytometry. New sensitivity test for various antineoplastic agents was also developed using surgical human specimens. The effects by interferon was judged by FITC labelling method.3. Sterile sorting method for NK cells and their antineoplastic effects. With use of monoclonal antibodies (Leu 7, Leu lla), a sterile sorting method for NK cells was established. Thus obtained NK cells were demonstrated in vitro to have cytocydal effects on the established brain tumor cell lines and cultured tumor cells obtained from brain tumor patients at surgery.5. Antineoplastic effects by a mixture of NK cells and IL-2. In establshed tumor cell lines, this mixture has the antineoplastic effects and clinical application of this method is now in progress.

以下是上述研究的总结。已出版专著5部，发表论文19篇，在医学会议上发表报告49次。脑肿瘤患者NK细胞动力学及NK细胞活性和T细胞亚群。在临床脑肿瘤病例中，采用单克隆抗体流式细胞术测定了患者术后和术前的T细胞亚群和NK细胞，证实了其作为评价患者免疫能力指标的可能性。NK活性也发挥着重要的指标作用。抗肿瘤药物与干扰素抗肿瘤作用的评价。采用流式细胞术对其体外抗肿瘤作用进行了评价。此外，还利用手术人体标本开发了各种抗肿瘤药物的新敏感性试验。采用FITC标记法判断干扰素的作用。NK细胞的无菌分选方法及其抗肿瘤作用。采用单克隆抗体（Leu 7、Leu lla）建立了NK细胞的无菌分选方法。因此，体外实验证明获得的NK细胞对已建立的脑肿瘤细胞系和体外培养的脑肿瘤患者手术获得的肿瘤细胞具有细胞质作用。NK细胞与IL-2混合作用的抗肿瘤作用。在已建立的肿瘤细胞系中，该混合物具有抗肿瘤作用，该方法的临床应用正在进行中。