Study on Antineoplastic Effects of NK Cells and IL-2 for Malignant Brain Tumors.

以下是上述研究的总结。取得的成果有5本书，19篇论文，以及49次医学会议上的演讲。脑肿瘤患者NK细胞、NK细胞活性及T细胞亚群的细胞动力学应用流式细胞术对临床脑肿瘤患者手术前后的T细胞亚群和NK细胞进行了检测，证实了其作为评价患者免疫功能的指标的可能性。自然资源活动作为指数也发挥了重要作用。抗肿瘤药物和干扰素的抗肿瘤效果评价。建立了一种利用流式细胞术进行体外抗肿瘤效果评价的方法。新的各种抗肿瘤药物的敏感性测试也被开发出来，使用的是外科人体标本。用FITC标记法判断干扰素的作用。自然杀伤细胞无菌分选方法及其抗肿瘤作用。用抗Leu-7、Leu-11a的单抗建立了NK细胞的无菌分选方法。所获得的NK细胞在体外对已建立的脑肿瘤细胞系和手术中获得的脑肿瘤患者的肿瘤细胞具有细胞周期效应。NK细胞和IL-2的混合抗肿瘤作用。在已建立的肿瘤细胞系中，该混合物具有抗肿瘤作用，该方法的临床应用目前正在进行中。

The following are the summary of the above said study. The achievements made are five books, 19 papers, and 49 presentations at medical meetings.1. Cell kinetics of the NK cells and NK activities and T cell subsets of brain tumor patients. In clinical cases of brain tumors, pre- and postoperative T cell subsets and NK cells were measured flow cytometrically by using monoclonal antibodies, and their possibility acting as the indices to evaluate the immunological capacity of these patients were proven. NK activities were also acting an important role as the index.2. Evaluation of the antineoplastic effects by antineoplastic agents and interferon. A method of in vitro evaluation of the antineoplastic effects was developed using flow cytometry. New sensitivity test for various antineoplastic agents was also developed using surgical human specimens. The effects by interferon was judged by FITC labelling method.3. Sterile sorting method for NK cells and their antineoplastic effects. With use of monoclonal antibodies (Leu 7, Leu lla), a sterile sorting method for NK cells was established. Thus obtained NK cells were demonstrated in vitro to have cytocydal effects on the established brain tumor cell lines and cultured tumor cells obtained from brain tumor patients at surgery.5. Antineoplastic effects by a mixture of NK cells and IL-2. In establshed tumor cell lines, this mixture has the antineoplastic effects and clinical application of this method is now in progress.