Control of cell cycle phase p130 gene transfaction indicating of cyclin expression in glioma.

细胞周期阶段 p130 基因转染的控制表明神经胶质瘤中细胞周期蛋白的表达。

• 批准号: 11671403
• 负责人: KAWAMOTO Keiji
• 金额: $ 2.3万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671403/
• 关键词: cyclin; brain tumor; Laser Scanning Cytometer; immunohistochemistry; Immunohistochemistry

Control of cell cycle phase by p130 gene transfaction indicating of cyclin expression in glioma.Expression of cyclin B1, D2, p53 with FITC labelled monoclonal antibodies was studied by Laser Scanning Cytometer (LSC) and immunohistochemistry in surgical meterials of glioma.1.Analysis of DNA ploidy with use of LSC showed the diploid pattern in low grade astrocytoma.2.DNA ploidy of malignant glioma revealed the aneuploidy pattern.3.Expression of cyclin D1 was correlated with the malignancy of glioma.4.The tumor edge showed high proliferating index and high expression of cyclin D1 from the diferent regions in malignant glioma.5.Expression of p53 did not reveal a definite difference in 3 different regions, but revealed the strong positive in malignant glioma.6.Umbiriical cord cells were expressed to labelled p130 monoclonal antibodies as a control and a part of glioma cells showed the positive expression.7.Plasmid of p130 was success to make and also was supplied from Kyusyu University, Prof. Hirano Labo. The plasmid was taken into the vector and transfected to the glioma MG87 cell line. The tumor cell was success to express the p130 gene, which was expected to control the glioma cell proliferation.

通过p130基因转染控制细胞周期阶段，表明胶质瘤中细胞周期蛋白的表达。通过激光扫描细胞仪（LSC）和免疫组化研究了FITC标记的单克隆抗体在胶质瘤手术材料中细胞周期蛋白B1、D2、p53的表达。1.利用LSC分析DNA倍性，显示低水平的二倍体模式。 2.恶性胶质瘤的DNA倍体性揭示了非整倍性模式。3.cyclin D1的表达与胶质瘤的恶性程度相关。4.肿瘤边缘呈现高增殖指数，且恶性胶质瘤不同区域的cyclin D1高表达。5.p53的表达在3个不同区域没有显示出明确的差异，但在胶质瘤中呈强阳性。 6.脐带细胞表达p130单克隆抗体作为对照，部分胶质瘤细胞呈阳性表达。7.p130质粒制作成功，由九州大学Hirano Labo教授提供。将质粒带入载体并转染至神经胶质瘤MG87细胞系。肿瘤细胞成功表达p130基因，有望控制胶质瘤细胞增殖。

项目成果

Zhao S, Kawamoto K, et al: "Flow cytometric and immunohistochemical detection of cyclins in Malignant Gliomas."Progress in Oncology. 9. 20-25 (1999)

赵 S、川本 K 等人：“恶性胶质瘤中细胞周期蛋白的流式细胞术和免疫组织化学检测”。肿瘤学进展。

Zhao S,Kawamoto K, et al.: "Flow cytometric and immunohistochemical detection of cyclins in Malignant Gliomas"Progress in Oncology. 9. 20-25 (1999)

赵S，Kawamoto K，等：“恶性胶质瘤中细胞周期蛋白的流式细胞术和免疫组织化学检测”肿瘤学进展。

Shiguang Zhao,Keiji Kawamoto,Yoshihiro Numa, et al.: "Immunohistochemical detection of p53, cyclins and DNA content analysis for judging of malignant glioma."Neuroimmunological Research. 12. 165-170 (1999)

赵世光，川本敬二，沼义宏，等：“免疫组化检测p53、细胞周期蛋白及DNA含量分析判断恶性胶质瘤。”神经免疫学研究。

Shiguang Zhao, Keiji Kawamoto, Yoshihiro Numa, et al: "Immunohistochemical detection of p53, cyclins and DNA content analysis for judging of malignant glioma."Neuroimmunological Research. 12. 165-170 (1999)

赵世光，Keiji Kawamoto，Yoshihiro Numa，等：“免疫组化检测p53、细胞周期蛋白和DNA含量分析用于判断恶性胶质瘤。”神经免疫学研究。

Yuji Tsukazaki, Yoshihiro Numa, et al: "Analysis of DNA-ploidy Using Laser Scanning Cytometer in Brain Tumors and its Clinical Application."Human Cell. 13. 221-228 (2000)

Yuji Tsukazaki、Yoshihiro Numa 等人：“使用激光扫描细胞仪分析脑肿瘤中的 DNA 倍体及其临床应用。”人类细胞。