Control of cell cycle phase p130 gene transfaction indicating of cyclin expression in glioma.

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Control of cell cycle phase by p130 gene transfaction indicating of cyclin expression in glioma.Expression of cyclin B1, D2, p53 with FITC labelled monoclonal antibodies was studied by Laser Scanning Cytometer (LSC) and immunohistochemistry in surgical meterials of glioma.1.Analysis of DNA ploidy with use of LSC showed the diploid pattern in low grade astrocytoma.2.DNA ploidy of malignant glioma revealed the aneuploidy pattern.3.Expression of cyclin D1 was correlated with the malignancy of glioma.4.The tumor edge showed high proliferating index and high expression of cyclin D1 from the diferent regions in malignant glioma.5.Expression of p53 did not reveal a definite difference in 3 different regions, but revealed the strong positive in malignant glioma.6.Umbiriical cord cells were expressed to labelled p130 monoclonal antibodies as a control and a part of glioma cells showed the positive expression.7.Plasmid of p130 was success to make and also was supplied from Kyusyu University, Prof. Hirano Labo. The plasmid was taken into the vector and transfected to the glioma MG87 cell line. The tumor cell was success to express the p130 gene, which was expected to control the glioma cell proliferation.