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FLOWCYTOMETRIC ANALYSES OF EXPRESSIONS OF GLYCOPROTEIN ANTIGEN AND EGF-RECEPTORS, AND DNA DISTRIBUTION IN BRAIN TUMORS

脑肿瘤中糖蛋白抗原和 EGF 受体的表达以及 DNA 分布的流式细胞术分析

基本信息

批准号：
02670640
负责人：
KAWAMOTO Keiji
金额：
$ 1.34万
依托单位：
KANSAI MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670640/
关键词：
brain tumor glycoprotein EGF EGF-receptor flow cytometry oncogene,DNA-histogram EGFーR FcM DNA Glyco protein flow cytometny brain tuwor

项目摘要

1)Flowcytometric analysis of the cell cycle in brain tumors:Flow cytometry (FCM) has been an effective method to grasp biological aspects of brain tumors. Of brain tumors, we have reported how primitive ectodermal tumor cells proliferate with the advance of passages of cell culture, and have proposed a new classification of ploidy patterns.2)Flowcytometric evaluation of action mechanism of antineoplastic agents:The effects of cisplatin and carboplatin appear at the S and G_2M phase. NK cells sorted with sterile technique have antineoplastic effects and the effects could be enhanced by addition of interleukin-1. The effects of lymphokine-activated killer cells were confirmed in vitro and the action mechanism was clarified.3)Expression of oncogene products in brain tumors:In established brain tumor cell strains, immunohistochemical and flowcytometric methods were useful for evaluation of the positivity of c-myc products expression.4)Expression of glycoprotein antigen in brain tumors:The subjects included an established KB cell line, brain tumors, lung cancers, uterus carcinomas, cancers in the head and neck. Of brain tumors, the expression was highly positive in metastatic tumors and epithelial cancers. FCM was useful in evaluating the expression.5)Expressions of EGF and EGF-R:The expressions differed according to the method of sample fixation. EGF expressed in various brain tumors, suggesting a correlation with malignancy. FCM was a simple and useful method for such study.

1)脑肿瘤细胞周期的流式细胞术分析：流式细胞术(FCM)是了解脑肿瘤生物学特性的有效方法。对于脑肿瘤，我们报道了原始外胚层肿瘤细胞是如何随着细胞培养代次的增加而增殖的，并提出了一个新的倍体模式分类。2)抗肿瘤药物作用机制的流式细胞仪评价：顺铂和卡铂的作用出现在S和G_2M期。无菌分选的NK细胞具有抗肿瘤作用，加入IL-1可增强其抗肿瘤作用。3)癌基因产物在脑肿瘤中的表达：在已建立的脑肿瘤细胞株中，免疫组织化学和流式细胞术有助于评估c-myc产物的阳性表达。4)脑肿瘤中糖蛋白抗原的表达：对象包括已建立的KB细胞系、脑肿瘤、肺癌、子宫癌、头颈部肿瘤。在脑肿瘤中，在转移性肿瘤和上皮性肿瘤中高表达。5)EGF和EGF-R的表达：根据标本固定的方法不同，其表达也不同。EGF在多种脑肿瘤中均有表达，提示EGF与肿瘤的恶性程度有关。流式细胞术是一种简便、实用的研究方法。