Study for neuronal regeneration in ischemic brain using bone marrow transplantation

骨髓移植促进缺血脑神经元再生的研究

• 批准号: 15591555
• 负责人: KAWAMOTO Keiji
• 金额: $ 2.18万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591555/
• 关键词: bone marrow; stem cell; transplantation; differentiation; f regeneration; Regeneration; bone marrow; ischemic brain; flow cytometry

The concept of neuronal regeneration has been rapidly changed from the past regenerative medicine. Recently neuronal differentiation and transplantation could be possible using by embryonal stem cell. Regeneration is much interested in the clinical field, so we studied for the possibility of neuronal regeneration in ischemic bran using bone marrow transplantation.Experiment of ischemic brainWe succeeded in modeling complete occulusion of ipsilatenal middle cerebral artery.Differentiation of bone marrow stem cells (BMC)BMS including the hematopoietic and mesenchymal cells could be differentiated into neuronal direction by adding PDGF-BB and/or b-FGF.Sorting and neuronal expression of CD34CD34- and CD34+ cells of lineage depleted BMC were sorted and neuronal differentiation showing lin-CD34-adult murine BMC were found by immunocytochemistry and PCR.Improvement of ischemic brainThe ischemic region was improved by transplantation of BMC.

神经元再生的概念与过去的再生医学相比已经发生了迅速的变化。近年来，胚胎干细胞的应用为神经元的分化和移植提供了可能。再生在临床领域非常感兴趣，脑缺血实验我们成功地建立了完全闭塞大脑中动脉的模型，通过加入PDGF-BB和/或B-β-CD，使骨髓干细胞（BMC）向神经元方向分化，包括造血细胞和间充质细胞。应用免疫细胞化学和PCR技术对去系小鼠骨髓基质细胞（BMC）中的CD_（34）CD_（34）-和CD_（34）+细胞进行分选和神经元表达的检测，发现骨髓基质细胞呈lin-CD_（34）-成体小鼠BMC向神经元分化。

项目成果

Neuronal and glial differentiation following culture of the human embryonic cortical stem cells

人胚胎皮质干细胞培养后的神经元和神经胶质分化

• 发表时间: 2003
• 期刊: Human cell 16
• 作者: Peng Liang;Zhao Shiguang;Keiji Kawamoto;et al.
• 通讯作者: et al.

LIANG P, KAWAMOTO K, et al.: "Neuronal and glial differentiation following culture of ES cells"HUMAN CELL. 16・3. 151-154 (2003)

LIANG P、KAWAMOTO K 等：“ES 细胞培养后的神经元和神经胶质分化”16・3（2003 年）。

Neuronal and glial differentiation following culture of ES cells

ES 细胞培养后的神经元和神经胶质分化

• 发表时间: 2003
• 期刊: 16
• 作者: LIANG P;KAWAMOTO K;et al.
• 通讯作者: et al.