Studies on DNA topoisomerase-targeted anti-cancer chemotherapy in gynecologic malignancy.

1.体外实验检测了新的喜树碱衍生物--干扰素-γ和喜树碱-11对两种人子宫内膜癌细胞株的增殖抑制作用。在两种细胞系中，干扰素-γ均增强了CPT-11.2的抗增殖活性。采用逆转录聚合酶链式反应(RT-PCR)方法检测不同癌细胞株DNA拓扑异构酶(TOPO)-I基因的表达。从肿瘤细胞系中分离出胞质多聚腺苷化RNA，用Topo-I特异性引物进行聚合酶链式反应，扩增出互补DNA。Topo-I抑制剂的细胞毒性与Topo-I的mRNA水平呈正相关。3.CPT-11和INF-γ的联合治疗将在荷瘤小鼠或大鼠身上进行试验。

1. The anti-proliferative effects of INF-gamma and CPT-11, a new derivative of camptothecin, on two human endometrial cancer cell lines were examined in vitro. In both cell lines, INF-gamma enhanced the anti-proliferative activity of CPT-11.2. Expression of DNA topoisomerase (Topo) -I mRNA in various cancer cell lines was detected using reverse transcription-polymerase chain reaction (RT-PCR) method. The cytoplasmic polyadenylated RNA isolated from cancer cell lines was reverse-transcribed and the complementary DNA was amplified by PCR primed with Topo-I specific primers. The level of Topo-I mRNA was correlated positively with the cytotoxicity of a Topo-I inhibitor.3.The combination therapy of CPT-11 and INF-gamma will be examined in tumor-bearing mice or rats.