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Basic mechanism of caloric response using isolated frog labyrinth

使用孤立的青蛙迷宫进行热量反应的基本机制

基本信息

批准号：
04671042
负责人：
SUZUKI Mamoru
金额：
$ 1.34万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Basic mechanism of caloric response was studied using isolated bull frog semicircular canal and utricle. First, the membranous labylinth was en bloc removed to study dynamic transduction of the endolymphatic flow. When the anterior canal was stimulated, an excitatory action potential was evoked from the anterior amullary nerve. At the same time, an inhibitory response was induced through the posterior nerve. Therefore, the endolymphatic flow applied to a part of the labyrinth leads to a systemic fluid shift in other organ through dynamic transduction. When an aminoglycoside drug was added to the perilymphatic space of the posterior semicircular canal, the action potential was inhibited. Therefore, ionic environment of the perilymph is essential for maintaining a function of the vestibular sensory cell. Finally, the semicircular canal and utricle were stimulated by thermoconvection, since caloric response is induced by thermoconvective fluid flow in the endolymph. Frozen Ringer's solution was used to produce thermoconvective flow along the gravitational plane. When a semicircular canal was stimulated by thermoconvection, excitatory and inhibotory responses were observed according to the direction of the thermoconvection. Therefore, thermoconvective flow serves as a valid mechanical stimulus to the sensory cells. When the utricle was stimulated by thermoconvection with different directions, the responses were the same regardless of the stimuli. This indicates that the utricle has an equal directional excitability, thus might be most influenced by weightless condition.

利用离体牛蛙半规管和椭圆囊研究了热反应的基本机制。首先，整体去除膜迷路以研究内淋巴流的动态转导。刺激前管时，前腹神经可诱发兴奋性动作电位。同时，通过后神经诱导抑制反应。因此，施加到迷路的一部分的内淋巴流通过动态转导导致其他器官中的全身流体转移。当将氨基糖苷类药物加入后半规管外淋巴间隙时，动作电位被抑制。因此，外淋巴液的离子环境对于维持前庭感觉细胞的功能是必不可少的。最后，半规管和椭圆囊被热对流刺激，因为热量反应是由内淋巴中的热对流流体流动引起的。冷冻林格氏液被用来产生热对流沿重力平面沿着。当半规管受到热对流刺激时，根据热对流的方向观察到兴奋性和兴奋性反应。因此，热对流作为一个有效的机械刺激的感觉细胞。当椭圆囊受到不同方向的热对流刺激时，无论何种刺激，其反应都是相同的。这表明椭圆果具有相同的方向兴奋性，可能受失重条件的影响最大。