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Mechanism of injury process and regeneration of the vestibular organ after drug intoxication.

药物中毒后前庭器官损伤过程及再生机制。

基本信息

批准号：
07671859
负责人：
SUZUKI Mamoru
金额：
$ 1.47万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671859/
关键词：
cupula isolated canal streptomycin posture recovery neurectomy nerve regeneration hair cell damage central compensation 抽出半規管平衡障害

项目摘要

The semicircular canal action potentials were recorded by changing potassium ion concentration. It turned out that the potentials stayd unchanged regardless whether or not the cupula is present, indicating the cupula does not serve as a barrier of ion to sensory cells. The action of streptomycin suflate (SM) on posture recovery was studied using adult bull frogs. SM intoxication resulted in various degrees of hair cell damage. Degree of posture recovery correlated well with the number of intact hair cell. The less the sensory cell damage, the better the posture recovery. The effect of SM on the regenerative process of the vestibular nerve was also studied. The vestibular nerve was sectioned in various conditions with intact endorgan or with SM intoxication. When the nerve was sectioned with the hair cells left intact, the nerve regenerated well and body balance recovered to normal in 8 to 9 weeks. However, when neural regeneration was blocked by inserting a piece of bone between the cut ends of nerve, recovery was incomplete. When the nerve was sectioned after damaging the hair cells, the nerve failed to regenerate and posture recovery was incomplete. These results suggest that the degree of posture recovery depends on hair cell function and neural regeneration. Furthermore, neural regeneration was strongly influenced by hair cell function. When the vestibular nerve was sectioned after complete posture recovery, the posture change was less and recovery time was shorter after the second neurectomy. This shows that in frogs central compensation could be easily established after the first neurectomy.

通过改变钾离子浓度记录半规管动作电位。结果表明，无论是否存在吸盘，电位都保持不变，表明吸盘对感觉细胞不起离子屏障作用。以成年牛蛙为实验动物，研究了硫酸链霉素（SM）对姿势恢复的作用。SM中毒导致不同程度的毛细胞损伤。姿势恢复的程度与毛细胞的完整数量相关。感觉细胞损伤越少，姿势恢复越好。同时观察SM对前庭神经再生过程的影响。前庭神经在不同条件下与完整的endorgan或SM中毒。当神经被切断，毛细胞保持完整时，神经再生良好，身体平衡在8 ~ 9周内恢复正常。然而，当神经再生被插入一片骨头之间的神经切断端，恢复是不完全的。当神经损伤毛细胞后被切断时，神经不能再生，姿势恢复不完全。这些结果表明，姿势恢复的程度取决于毛细胞功能和神经再生。此外，毛细胞功能强烈影响神经再生。完全恢复姿势后再切断前庭神经，第二次切断后姿势改变少，恢复时间短。这表明，在青蛙中，在第一次神经切除术后，可以很容易地建立中央补偿。