Establishment of Analytical method of Binding Media Used Works of Art

艺术品装订介质分析方法的建立

• 批准号: 05835004
• 负责人: INABA Masamitsu
• 金额: $ 1.22万
• 依托单位: Tokyo National University of Fine Arts and Music
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05835004/
• 关键词: Binding media; GC-MS; Methylation; Amino acid; Analysis; Fatty acid; Protein; Paintings; タンバク質

For the study of art objects and their rostoration, the identification of binding media as well as that of pigments is considerably important. Binding media consist of complicated organic compounds but only a small amount of sample can be used for analysis. In this study, identification of binding media such as drying oils and glue used in works of art was carried out by GC-MS and by HPLC.The application of two organic alkaline reagent, tetramethylammonium hydroxide (TMAH) and m-trifluoro-methylphenyltrimethylammonium hydroxide (m-TFPTAH) , as the reagent for the identification of drying oil in the dried paint films with GC-MS is studied. Both two reagents showed good results for obtaining the Palmitic/Stearic ratios from various oils, comparing with the "diazomethane methylation method" , but TMAH required higher temperature for methylation. Therefore, m-TFPTAH seemed to be superior in the purpose, and is supposed to make possible to identify the drying oil from smaller sample with simpler method than diazomethane methylation. Infuence of the quality of binding media used in ancient wall paintings of Dunhuang Mogao-ku Grottoes upon damages of their surfaces were studied by amino acid analysis with HPLC tequnique. A very small portion of each sample taken from the surfaces of such wall paintings was hydrolyzed by Hcl gas phase method. The hydrolytic product was injected into HPLC column to identify and determine theu composition of different kinds of amino acid. Amino acid similarity of each sample was Obtained from the pattern of each amino acid ratio between of paint samples and that of controls, and also compartion of amino acid composition of each sample was calculated by Cluster analysis. From the results of twenty paintsamples, presence of animal glue components in every sample and their amounts were caluclated in terms of total quantities of amino acid.

对于艺术品及其保存的研究，结合介质的识别以及颜料的识别是相当重要的。结合介质由复杂的有机化合物组成，但只能用少量的样品进行分析。本研究采用GC-MS和HPLC对艺术品中的干油、胶水等粘合剂进行了鉴定，并研究了四甲基氢氧化铵(TMAH)和间三氟甲基苯基三甲基氢氧化铵(m-TFPTAH)两种有机碱性试剂作为干漆膜干油的GC-MS鉴定试剂的应用。与“重氮甲烷甲基化法”相比，两种试剂对不同油品的棕榈酸/硬脂酸比值均有较好的测定结果，但TMAH的甲基化需要较高的温度。因此，m-TFPTAH比重氮甲烷甲基化更适合于从小样品中鉴别干燥油。采用高效液相色谱仪进行氨基酸分析，研究了敦煌莫高窟古壁画粘结剂的质量对其表面损伤的影响。从这种壁画表面提取的每个样品的非常小的一部分被HCl气相方法水解。将水解物注入高效液相色谱柱，鉴定和测定不同种类氨基酸的组成。根据涂料样品与对照样品各氨基酸的比值模式，得到各样品的氨基酸相似度，并通过聚类分析计算各样品的氨基酸组成比较。从20个油漆样品的结果中，计算出每个样品中动物胶组分的存在及其数量是按氨基酸总量计算的。