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Evaluation of dietary plants for superoxide generation (or its inhibition), or scavening activities, and their active constituents

膳食植物超氧化物生成（或其抑制）或清除活性及其活性成分的评估

基本信息

批准号：
06660156
负责人：
OHIGASHI Hajime
金额：
$ 1.28万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Super oxide anion radical (SO), the initial active oxygene species, is known to be very important in the primary metabolisms and also physiological functions in biological systems. The excess generation of SO is, however, closely related to ageing and carcinogenesis. In addition to the enzymatic (SOD) heterolytic reaction, the control of SO by food constituents may be important in our daily life. We have already established assay methods for SO generation or scavenging using an enzyme, xanthine oxidase, or HL-60 cells. By these methods, some significant dietary plants from tropical south-east Asia and also from Japan have been found. 1'-Acetoxychavicol acetate (ACA) from Languas galanga, a highly potent anti-tumor promoter, has been estimated to be a strong inhibitor of xanthine oxidase. It also inhibited TPA-stimulated SO generation in HL-60 cells differentiated with DMSO.The SO generation inhibition was observed by a flow cytemetory to also occur inside the cells. SO generation had been known to be related to anti-tumor promotion. Then, inhibition of SO generation was proposed to be one of the mechanisms of ACA for anti-tumor promotion. (R) -Rosmarinic acid was isolated from green perilla as an SO scavenging agent. Interestingly, it did not inhibit Epstein-Barrvirus activation induced by a tumor promoter, which has been known to well correlated to anti-tumor promotion. Further studies on these activities must present us more important information about the relationship between SO and carcinogenesis.

超氧阴离子自由基（SO）是一种初始活性氧，在生物系统的初级代谢和生理功能中起着重要作用。然而，过量生成SO与衰老和致癌密切相关。除了酶（SOD）异解反应外，食物成分对SO的控制在我们的日常生活中可能很重要。我们已经建立了使用酶、黄嘌呤氧化酶或HL-60细胞产生或清除SO的测定方法。通过这些方法，发现了来自热带东南亚和日本的一些重要的膳食植物。高良姜中的1′-乙酰基chavicol醋酸酯（ACA）是一种高效的抗肿瘤启动子，被认为是黄嘌呤氧化酶的强抑制剂。它还能抑制tpa刺激的DMSO分化的HL-60细胞的SO生成。流式细胞仪观察到这种抑制作用也发生在细胞内。已知SO的产生与抗肿瘤促进有关。进而提出抑制SO生成可能是ACA抗肿瘤促进作用的机制之一。(R) -迷迭香酸是从绿紫苏中分离得到的一种SO清除剂。有趣的是，它没有抑制肿瘤启动子诱导的Epstein-Barrvirus激活，而肿瘤启动子与抗肿瘤促进密切相关。对这些活动的进一步研究必须为我们提供更多关于SO与致癌之间关系的重要信息。