The diagnosis of resinous disease of Hinoki (Chamaecyparis obtusa) by constituent compounds of resin

从树脂成分诊断扁柏树脂病

• 批准号: 06660173
• 负责人: YAMAMOTO Hiroshi
• 金额: $ 1.22万
• 依托单位: IBARAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660173/
• 关键词: resinous disease; Chamaecyparis obtusa; resin; diterpen; gas chromatography; labdane diterpen; growth assay; bark extravtives; 漏脂症; テルペン; 植物病害

(1) Purpose : The resinous disease of Hinoki was widely recorded from Kushu and Honshu of Japan. The lesions of the disease distribute on the lower part of stem which provides commercial log. The establishment of a diagnosis of disease by constituent compounds of resin was examined for prevention of the disease.(2) Results :1) Terpens isolated from the resin of resinous diseased Hinoki.A grest quantities of resin was extracted with hexane and ethyl acetate from bark of the diseased part. The hexane eatract of diseased part was 40 times and ethyl acetate extact was 8 times that of uninfected part. The bark of a few meter higher part from the lesion looked healthy, but these extracts were 1.5 times and 1.7 times that of uninfected part. Five diterpens were separated by chromatographys from the hexane extract and identified by physical data : manool (III,8.9%), torulosol (II,2.6%), tolulosal (I,1.3%), communic acid (IV,4.1%), and ferruginol (V,5.7%). IV and V were two main diterpens from … More cones of Hinoki. I,II,III were found for the first time from Hinoki.2) Growth assay of three causual fungi against isolated diterpens.A 5mm-diam.disc of fungi was placed in the center of filter paper adsorbed 1mg diterpen on PDA plate. The growth of fungi was measured and the fungal activity was calculated by the difference in growth of the fungi on the control. The growth of Cryptosporiopsis abietina was inhibited by I,II and III.Five diterpens had no effect on the growth of Sarea resinae. The growth of Cistella japonica was inhibited by II and I.3) The diagnosis of resinous disease of Hinoki.Diterpens I,II and III are characteristic compounds of the disease, because they were found in the diseased resin and inhibited two causal fungi. It may be concluded that increases of these diterpens can judge the degree of the resinous disease. In analytical process of gas chromatography using a polor capillary column, one strong signal for decomposed product of uninjured bark indicates uninfected condition of Hinoki, 4 or 5 signals of manool and others indicates infected condition. Less

(1)目的：扁柏病广泛分布于日本九州和本州。病害主要分布在提供商品材的树干下部。为了预防疾病，检查了通过树脂的组成化合物诊断疾病的建立。(2)结果：1）从患病扁柏树脂中分离得到萜类化合物，其中以正己烷和乙酸乙酯提取的含量最高。病部正己烷提取物是未感染部位的40倍，乙酸乙酯提取物是未感染部位的8倍。离病斑几米高的部位树皮看起来很健康，但这些提取物是未感染部位的1.5倍和1.7倍。从正己烷提取物中分离得到5种二萜类化合物，分别为：异戊醇（Ⅲ，8.9%）、赤藓醇（Ⅱ，2.6%）、甲苯柳醛（Ⅰ，1.3%）、communic acid（Ⅳ，4.1%）和ferruginol（Ⅴ，5.7%）。IV和V是两个主要的差异， ...更多信息 扁柏的球果（2）三种真菌对分离的二萜类化合物的生长测定：在PDA平板上吸附1 mg二萜类化合物的滤纸上，放置一个直径为5 mm的菌盘，在滤纸中心放置一个菌盘。测量真菌的生长，并通过真菌在对照上的生长差异计算真菌活性。Ⅰ、Ⅱ、Ⅲ对冷杉隐孢子虫的生长有抑制作用，五种二萜类化合物对香茶菜的生长无影响。Ⅱ和Ⅰ对日本池藻的生长有抑制作用。3）扁柏枯病的诊断。二萜类化合物Ⅰ、Ⅱ和Ⅲ是扁柏枯病的特征化合物，它们存在于病树中，对两种病原真菌有抑制作用。因此，这些二萜类化合物的增加可作为判断本病严重程度的指标。在气相色谱分析过程中，用Polor毛细管柱，未受害树皮的分解产物中有一个强信号表示扁柏未感染，有4 ~ 5个强信号表示扁柏醇，其它信号表示扁柏感染。少