The diagnosis of resinous disease of Hinoki (Chamaecyparis obtusa) by constituent compounds of resin

(1)目的：日本久州岛和本州岛广泛记录了Hinoki树脂病。病斑分布在茎的下部，为商品原木提供用材。(2)结果：1)用正己烷和乙酸乙酯从树脂病部位的树皮中分离出少量的树脂。病区正己烷消耗量是非病区的40倍，乙酸乙酯萃取物是非病区的8倍。离病斑几米高的树皮看起来很健康，但这些提取物是未感染部位的1.5倍和1.7倍。从正己烷提取物中分离得到5个二萜类化合物：甘露醇(III，8.9%)、托鲁洛松(II，2.6%)、甲苯磺酸(I，1.3%)、交链酸(IV，4.1%)、铁锈醇(V，5.7%)。IV和V是…中的两个主要二萜类化合物更多的Hinoki圆锥体。从Hinoki中首次发现I，II，III。2)三种常见真菌对分离的二萜类真菌的生长试验，将直径5 mm的真菌圆盘放在PDA平板上吸附1 mg二萜的滤纸中央。测定真菌的生长情况，并根据对照真菌的生长差异计算真菌活性。I、II和III对隐孢子虫的生长有抑制作用。5种二萜类化合物对树脂隐孢子虫的生长没有影响。3)诊断日本肉毒杆菌树脂性疾病。二萜I、II和III是该病的特征化合物，因为它们存在于患病的树脂中，对两种病原菌有抑制作用。可以认为，这些二萜类物质的增加可以判断树脂性疾病的程度。在Polor毛细管色谱柱的分析过程中，未受伤害的树皮分解产物的1个强信号表示Hinoki未受感染，4或5个甘露醇信号表示有感染情况，其他信号表示有感染情况。较少

(1) Purpose : The resinous disease of Hinoki was widely recorded from Kushu and Honshu of Japan. The lesions of the disease distribute on the lower part of stem which provides commercial log. The establishment of a diagnosis of disease by constituent compounds of resin was examined for prevention of the disease.(2) Results :1) Terpens isolated from the resin of resinous diseased Hinoki.A grest quantities of resin was extracted with hexane and ethyl acetate from bark of the diseased part. The hexane eatract of diseased part was 40 times and ethyl acetate extact was 8 times that of uninfected part. The bark of a few meter higher part from the lesion looked healthy, but these extracts were 1.5 times and 1.7 times that of uninfected part. Five diterpens were separated by chromatographys from the hexane extract and identified by physical data : manool (III,8.9%), torulosol (II,2.6%), tolulosal (I,1.3%), communic acid (IV,4.1%), and ferruginol (V,5.7%). IV and V were two main diterpens from … More cones of Hinoki. I,II,III were found for the first time from Hinoki.2) Growth assay of three causual fungi against isolated diterpens.A 5mm-diam.disc of fungi was placed in the center of filter paper adsorbed 1mg diterpen on PDA plate. The growth of fungi was measured and the fungal activity was calculated by the difference in growth of the fungi on the control. The growth of Cryptosporiopsis abietina was inhibited by I,II and III.Five diterpens had no effect on the growth of Sarea resinae. The growth of Cistella japonica was inhibited by II and I.3) The diagnosis of resinous disease of Hinoki.Diterpens I,II and III are characteristic compounds of the disease, because they were found in the diseased resin and inhibited two causal fungi. It may be concluded that increases of these diterpens can judge the degree of the resinous disease. In analytical process of gas chromatography using a polor capillary column, one strong signal for decomposed product of uninjured bark indicates uninfected condition of Hinoki, 4 or 5 signals of manool and others indicates infected condition. Less