Clinicopathologic, molecular pathologic and biologic study of malignant lymphoma

恶性淋巴瘤的临床病理学、分子病理学和生物学研究

• 批准号: 06670211
• 负责人: NAKAMURA Shigeo
• 金额: $ 1.34万
• 依托单位: Aichi Cancer Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670211/
• 关键词: Malignant Lymphoma; Molecular Pathology; Mantle Cell Lymphoma; Cyclin D1; Primary Gastric Lymphoma; Angiocentric Lymphoma; CD56 (NCAM); Epstein-Barr Virus; 人体病理学; cyclin Dl; 鼻腔T; NK細胞リンパ腫; 境界領域的リンパ増殖病変; 生物学; cyclin D1 蛋白質; bcl-1; PRAD1; n

Several entities of non-Hodgkin's lymphoma, i.e., mantle cell lymphoma (MCL), primary gastric B-cell lymphoma (PGL), CD56 (NCAM) -positive angiocentric lymphoma (AcL), and nasal T/NK-cell lymphomas (NTCL), were investigated on their clinicopathologic, molecular pathologic and biologic aspects. We produced a monoclonal antibody, 5D4, against a recombinant PRAD1/cyclin D1 product, and revealed that the immunolocalization of cyclin D1 protein is an essential marker for the definite diagnosis of MCL.The patients with cyclin D1 overexpressing lymphomas appeared to consitute a very homogeneous group, the 5-year survival rate being 11%. The histological typing of PGL,which was proposed by us on the basis of the concept of low-grade B-cell lymphoma of mucosa-associated lymphoid tissue (MALT), showed to correlate with growth appearance, pathologic stage, prognosis, and expression of several oncogenes such as bc12 and p53.10 patients with CD56-positive AcLs were also identified among the registered cases in Aichi Cancer Center, constituting a distinct group characterized by angiocentricity, azurophilic granules, putative natural killer (NK) -cell lineage, and aggressive behavior. Furthermore, the present study supported that NTCL is identified as Epstein-Barr virus-associated tumors distinct from the other T-cell lymphoma. These date may provide the basis for clarifying the disease spectrum of malignant lymphomas in the Japanese patients. During the process of this study, further investigation of lymphoproliferative disorders located at the gray zone was also emphasized.

几种非霍奇金淋巴瘤，即，本文对胃套细胞淋巴瘤（MCL）、原发性胃B细胞淋巴瘤（PGL）、CD 56（NCAM）阳性血管中心性淋巴瘤（AcL）和鼻T/NK细胞淋巴瘤（NTCL）的临床病理、分子病理和生物学特征进行了研究。我们制备了抗PRAD 1/cyclin D1重组产物的单克隆抗体5D4，发现cyclin D1蛋白的免疫定位是MCL确诊的一个重要标志，cyclin D1过表达的淋巴瘤患者构成一个非常均一的群体，5年生存率为11%。PGL的组织学分型是我们根据粘膜相关淋巴组织（MALT）的低度B细胞淋巴瘤的概念提出的，它显示出与生长外观、病理分期、预后以及几种癌基因如bc 12和p53的表达相关。在爱知癌症中心的登记病例中还发现了10例CD 56阳性AcLs患者，构成了一个独特的组，其特征在于血管中心性、嗜天青颗粒、推定的自然杀伤（NK）细胞谱系和攻击行为。此外，本研究支持NTCL被鉴定为与其他T细胞淋巴瘤不同的EB病毒相关肿瘤。这些数据可能为阐明日本患者恶性淋巴瘤的疾病谱提供依据。在本研究的过程中，还强调了对位于灰区的淋巴组织增生性疾病的进一步研究。

项目成果

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Nakamura S 等人：“侵袭性自然杀伤细胞淋巴瘤病例。”

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Kojima M, et al.: "Centroblastic and centroblastic-centrocytic lymphomas with prominent epithelioid....." Hum Pathol. (in press). (1996)

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